Effect of Hexane Extract of Galla Rhois on Inflammatory Alveolar Bone Loss
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
One of functions of Galla Rhois (GR) is reportedly an anti-inflammatory effect on the several inflammatory diseases. However, an effect of GR related to periodontitis has not been investigated. In the present study, we examined the effect of the hexane extract of Galla Rhois (GR-H) on periodontitis. Cytotoxicity was assessed by MTS analysis using human gingival fibroblast (hGF) cells. Experimental periodontitis was induced by injecting E.coli LPS into the palatal gingiva maxillary molar thrice weekly for 3 weeks (LPS group). GR-H diluted in 1xPBS was orally administrated using a syringe at 30 mg/kg body weight and 100 mg/kg body weight once a day (GR-H group). GR-H effect on the alveolar bone loss (ABL) was digitized with a micro-CT. GR-H treatment at concentrations exceeding 0.5mg/ml showed cytotoxic effect in hGF cells. The micro-CT among groups were presented for the different distances from cemento-enamel junction (CEJ) to alveolar bone crest (ABC). The results indicated an inhibitory effect on alveolar bone loss for orally administered GR-H in a model of LPS-induced periodontitis.
Abstract
One of functions of Galla Rhois (GR) is reportedly an anti-inflammatory effect on the several inflammatory diseases. However, an effect of GR related to periodontitis has not been investigated. In the present study, we examined the effect of the hexane extract of Galla Rhois (GR-H) on periodontitis. Cytotoxicity was assessed by MTS analysis using human gingival fibroblast (hGF) cells. Experimental periodontitis was induced by injecting E.coli LPS into the palatal gingiva maxillary molar thrice weekly for 3 weeks (LPS group). GR-H diluted in 1xPBS was orally administrated using a syringe at 30 mg/kg body weight and 100 mg/kg body weight once a day (GR-H group). GR-H effect on the alveolar bone loss (ABL) was digitized with a micro-CT. GR-H treatment at concentrations exceeding 0.5mg/ml showed cytotoxic effect in hGF cells. The micro-CT among groups were presented for the different distances from cemento-enamel junction (CEJ) to alveolar bone crest (ABC). The results indicated an inhibitory effect on alveolar bone loss for orally administered GR-H in a model of LPS-induced periodontitis.
참고문헌 (27)
- Lohinai Z, "Evidence for the expression of cyclooxygenase-2enzyme in periodontitis", Life Sci, 70, pp.279~290 (2001)
- Vane JR, "Cycloxygenase 1 and 2", Annu Rev Pharmacol Toxicol, 38, pp.97~120 (1998)
- Palombo EA, "Traditional medicinal plant extracts and natural products with activity against oral bacteria: potential application in the prevention and treatment of oral diseases", Evid Based Complement Alternat Med, 2011, pp.1~15 (2011)[DOI]
- Odilon Djakpo, "Rhus chinensis and Galla Chinensis - folklore to modern evidence: review", Phytotherapy Research, 24(12), pp.1739~1747 (2010), Wiley-Blackwell[DOI]
- Jaw-Chyun Chen, "Identification of Escherichia coli enterotoxin inhibitors from traditional medicinal herbs by in silico, in vitro, and in vivo analyses", Journal of Ethnopharmacology, 121(3), pp.372~378 (2009), Elsevier BV[DOI]
- Tian F, "Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis: The polarity affects the bioactivities", Food chemistry, 113, pp.173~179 (2009)
- 강미선, "Inhibitory Effect of Methyl Gallate and Gallic Acid on Oral Bacteria", The Journal of Microbiology, 46(6), pp.744~750 (2008), 한국미생물학회
- Liu Z, "The effect of Galla chinensis on the demineralization of enamel", Sichuan Da Xue Xue Bao Yi Xue Ban, 34, pp.507~509 (2003)
- Ali Cekici, "Inflammatory and immune pathways in the pathogenesis of periodontal disease", Periodontology 2000, 64(1), pp.57~80 (2014), Wiley-Blackwell[DOI]
- Patil C, "Actinobacillus actinomycetemcomitans lipopolysaccharide induces interleukin‐6 expression through multiple mitogenactivated protein kinase pathways in periodontal ligament fibroblasts", Oral microbiology and immunology, 21, pp.392~400 (2006)
- Toshiaki Ara, "Human gingival fibroblasts are critical in sustaining inflammation in periodontal disease", Journal of Periodontal Research, 44(1), pp.21~27 (2009), Wiley-Blackwell[DOI]
- Lei Ren, "The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease", Journal of Clinical Periodontology, 36(8), pp.619~626 (2009), Wiley-Blackwell[DOI]
- J.-P. Li, "Differential expression of Toll-like receptor 4 in healthy and diseased human gingiva", Journal of Periodontal Research, 49(6), pp.845~854 (2014), Wiley-Blackwell[DOI]
- Kim SH, "The anti-anaphylactic effect of the gall of Rhus javanica is mediated through inhibition of histamine release and inflammatory cytokine secretion", Int Immunopharmacol, 5, pp.1820~1829 (2005)
- 박필훈, "Involvement of Heme Oxygenase-1 Induction in Inhibitory Effect of Ethyl Gallate Isolated from Galla Rhois on Nitric Oxide Production in RAW 264.7 Macrophages", Archives of Pharmacal Research, 34(9), pp.1545~1552 (2011), 대한약학회
- Hasturk H, "RvE1 protects from local inflammation and osteoclast-mediated bone destruction in periodontitis", FASEB J, 20, pp.401~403 (2006)
- Chae HS, "Methyl gallate inhibits the production of interleukin-6 and nitric oxide via down-regulation of extracellular-signal regulated protein kinase in RAW 264.7 cells", Am J Chin Med, 38, pp.973~983 (2010)
- Bo-Ah Lee, "The Effects of a Novel Botanical Agent on Lipopolysaccharide-Induced Alveolar Bone Loss in Rats", Journal of Periodontology, 84(8), pp.1221~1229 (2013), American Academy of Periodontology (AAP)[DOI]
- Morgana R Guimarães, "Curcumin modulates the immune response associated with LPS-induced periodontal disease in rats", Innate Immunity, 18(1), pp.155~163 (2012), SAGE Publications[DOI]
- Park CH, "Three-dimensional micro-computed tomographic imaging of alveolar bone in experimental bone loss or repair", J Periodontol, 78, pp.273~281 (2007)
- Ali Gürkan, "Therapeutic efficacy of vasoactive intestinal peptide in escherichia coli lipopolysaccharideinduced experimental periodontitis in rats", Journal of Periodontology, 80(10), pp.1655~1664 (2009), American Academy of Periodontology (AAP)[DOI]
- Eizo Yamano, "Inhibitory effects of orally administrated liposomal bovine lactoferrin on the LPS-induced osteoclastogenesis", Laboratory Investigation, 90(8), pp.1236~1246 (2010), Springer Nature[DOI]
- Hamada Y, "Application of limited cone beam computed tomography to clinical assessment of alveolar bone grafting: a preliminary report", Cleft Palate Craniofac J, 42, pp.128~137 (2005)
- Van Oosterwyck H, "The use of microfocus computerized tomography as a new technique for characterizing bone tissue around oral implants", J Oral Implantol, 26, pp.5~12 (2000)
- Mengel R, "Digital volume tomography in the diagnosis of periodontal defects: an in vitro study on native pig and human mandibles", J Periodontol, 76, pp.665~673 (2005)
- H Ebina, "Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects", Oral Diseases, 15(4), pp.273~280 (2009), Wiley-Blackwell[DOI]
- Wada T, "RANKL–RANK signaling in osteoclastogenesis and bone disease", Trends Mol Med, 12, pp.17~25 (2006)
