Inhibitory Effect of Standardized Curcuma xanthorrhiza Supercritical Extract on LPS-Induced Periodontitis in Rats
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Periodontitis, which is a severe inflammatory disease caused by endotoxins secreted from oral pathogens, destructs gingival tissue and alveolar bone. Curcuma xanthorrhiza, commonly called Java turmeric, has been shown to possess anti-bacterial and anti-inflammatory activities. The present study evaluated the inhibitory effect of C. xanthorrhiza supercritical extract (CXS) standardized with xanthorrhizol on lipopolysaccharide (LPS)-induced periodontitis in an animal model. LPS was topically injected into the periodontium of Sprague-Dawley rats to induce periodontitis and CXS (30 and 100 mg·kg-1·day-1) was orally administered after day 12. Histologically, CXS inhibited the collapse of gingival tissue by preventing cell infiltration. CXS significantly downregulated the expression of matrix metalloproteases (MMPs) and inflammation-related biomarkers, such as nuclear factor-kappa B (NF-κB) and interleukin-1 beta (IL-1β) in gingival tissue. CXS also improved bone remodeling by downregulating osteoclastic transcription factors, such as nuclear factor of activated T-cells c1 (NFATc1), tartrate-resistant acid phosphatase (TRAP), and cathepsin K. In addition, CXS upregulated osteoblast differentiation-related markers, alkaline phosphate (ALP) and collagen type I alpha (COLA1). Thus, CXS can ameliorate periodontitis by inhibiting inflammation and improving bone remodeling.
Abstract
Periodontitis, which is a severe inflammatory disease caused by endotoxins secreted from oral pathogens, destructs gingival tissue and alveolar bone. Curcuma xanthorrhiza, commonly called Java turmeric, has been shown to possess anti-bacterial and anti-inflammatory activities. The present study evaluated the inhibitory effect of C. xanthorrhiza supercritical extract (CXS) standardized with xanthorrhizol on lipopolysaccharide (LPS)-induced periodontitis in an animal model. LPS was topically injected into the periodontium of Sprague-Dawley rats to induce periodontitis and CXS (30 and 100 mg·kg-1·day-1) was orally administered after day 12. Histologically, CXS inhibited the collapse of gingival tissue by preventing cell infiltration. CXS significantly downregulated the expression of matrix metalloproteases (MMPs) and inflammation-related biomarkers, such as nuclear factor-kappa B (NF-κB) and interleukin-1 beta (IL-1β) in gingival tissue. CXS also improved bone remodeling by downregulating osteoclastic transcription factors, such as nuclear factor of activated T-cells c1 (NFATc1), tartrate-resistant acid phosphatase (TRAP), and cathepsin K. In addition, CXS upregulated osteoblast differentiation-related markers, alkaline phosphate (ALP) and collagen type I alpha (COLA1). Thus, CXS can ameliorate periodontitis by inhibiting inflammation and improving bone remodeling.
참고문헌 (36)
- Cekici A, "Inflammatory and immune pathways in the pathogenesis of periodontal disease", Periodontol. 2000, 64, pp.57~80 (2014)
- Singh N, "Effect of scaling & root planing on the activity of ALP in GCF & serum of patients with gingivitis, chronic and aggressive periodontitis: a comparative study", J. Oral Biol. Craniofac. Res, pp.123~126 (2017)
- Hajishengallis G., "Periodontitis: from microbial immune subversion to systemic inflammation", Nat. Rev. Immunol, 15, pp.30~44 (2015)
- Barton MK., "Evidence accumulates indicating periodontal disease as a risk factor for colorectal cancer or lymphoma", CA Cancer J. Clin, 67, pp.173~174 (2017)
- Merchant AT, "Evaluating periodontal treatment to prevent Cardiovascular disease: challenges and possible solutions", Curr. Atheroscler. Rep, 19, pp.4~9 (2017)
- Harding A, "Can better management of periodontal disease delay the onset and progression of Alzheimer’s disease?", J. Alzheimers Dis, pp.337~348 (2017)
- Hasan A, "A clinical guide to periodontology:pathology of periodontal disease", Br. Dent. J, 216, pp.457~461 (2014)
- Chiu HC, "Effect of high glucose, Porphyromonas gingivalis lipopolysaccharide and advanced glycation end-products on production of interleukin-6", -8 by gingival fibroblasts, 2017, pp.52, 268~276
- Hienz SA, "Mechanisms of bone resorption in periodontitis", J. Immunol. Res, pp.615486~ (2015)
- Müller HD, "Salivary pellets induce a pro-inflammatory response involving the TLR4–NF-kB pathway in gingival fibroblasts", BMC Oral Health, 17, pp.15~24 (2016)
- Franco C, "Matrix metalloproteinases as regulators of periodontal inflammation", Int. J. Mol. Sci, 18, pp.440~451 (2017)
- Maeso G, "Levels of metalloproteinase-2 and-9 and tissue inhibitor of matrix metalloproteinase-1 in gingival crevicular fluid of patients with periodontitis, gingivitis, and healthy gingiva", Quintessence Int, 38, pp.247~252 (2007)
- Scannapieco FA., "Periodontal inflammation: from gingivitis to systemic disease?", Compend. Contin. Educ. Dent, 25, pp.16~25 (2004)
- He X, "Resveratrol prevents RANKL-induced osteoclast differentiation of murine osteoclast progenitor RAW 264.7 cells through inhibition of ROS production", Biophys. Res. Commun, 401, pp.356~362 (2010)
- Algate K, "The effects of tumour necrosis factor-α on bone cells involved in periodontal alveolar bone loss; osteoclasts, osteoblasts and osteocytes", J. Periodontal Res, 51, pp.549~566 (2016)
- Jung-Eun Kim, "Antibacterial Characteristics of Curcuma xanthorrhiza Extract on Streptococcus mutans Biofilm", The Journal of Microbiology, 46(2), pp.228~232 (2008), 한국미생물학회
- Mary HP, "Phytochemical characterization and antimicrobial activity of Curcuma xanthorrhiza Roxb", Asian Pac. J. Trop. Biomed, 2, pp.S637~S640 (2012)
- 김백일, "Curcuma xanthorrhiza 추출물 및 함유 치약의 구취 억제 효과와 구강 유해균에 대한 선택적 항균 효과", 대한구강보건학회지, 29(2), pp.222~237 (2005), 대한예방치과·구강보건학회
- Cho JY, "Standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza prevents murine ulcerative colitis by regulation of inflammation", J. Funct. Foods, 30, pp.282~289 (2017)
- Ab Halim MR, "Standardization and phytochemical studies of Curcuma xanthorrhiza Roxb", Int. J. Pharm. Pharm. Sci, 4, pp.606~610 (2012)
- Oon SF, "Xanthorrhizol: a review of its pharmacological activities and anticancer properties", Cancer Cell Int, 15, pp.100~114 (2015)
- 서태근, "Porphyromonas gingivalis-Derived Lipopolysaccharide-Mediated Activation of MAPK Signaling Regulates Inflammatory Response and Differentiation in Human Periodontal Ligament Fibroblasts", The Journal of Microbiology, 50(2), pp.311~319 (2012), 한국미생물학회[DOI]
- Walsh MC, "Biology of the RAN KL–RAN K–OPG system in immunity, bone, and beyond", Front. Immunol, pp.511~521 (2014)
- Xu T, "The function of naringin in inducing secretion of osteoprotegerin and inhibiting formation of osteoclasts. Evid. Based Complement", Alternat. Med, pp.8981650~ (2016)
- Ehrnhöfer-Ressler MM, "Identification of 1, 8-cineole, borneol, camphor, and thujone as anti-inflammatory compounds in a Salvia officinalis L. infusion using human gingival fibroblasts", J. Agric. Food Chem, 61, pp.3451~3459 (2013)
- Hu P, "Curcumin attenuates cyclooxygenase-2 expression via inhibition of the NF-κB pathway in lipopolysaccharide-stimulated human gingival fibroblasts", Cell Biol. Int, 37, pp.443~448 (2013)
- Rufino AT, "Anti-inflammatory and chondroprotective activity of (+)-α-pinene: structural and enantiomeric selectivity", J. Nat. Prod, 77, pp.264~269 (2014)
- Lim CS, "Antioxidant and antiinflammatory activities of xanthorrhizol in hippocampal neurons and primary cultured microglia", J. Neurosci. Res, 82, pp.831~838 (2005)
- 고선일, "Inhibitory Effect of Myricetin on Matrix Metalloproteinase Expression and Activity in Periodontal Inflammation", International Journal of Oral Biology, 41(4), pp.163~173 (2016), 대한구강생물학회
- Lee K, "Selective regulation of MAPK signaling media ANKLtes R-dependent osteoclast differentiation", Int. J. Biol. Sci, 12, pp.235~245 (2016)
- Li Q, "MAPK usage in periodontal disease progression", J. Signal Transduct, pp.308943~ (2012)
- Kostenuik PJ., "Osteoprotegerin and RANKL regulate bone resorption, density, geometry and strength", Curr. Opin. Pharmacol, 5, pp.618~625 (2005)
- Ram VS, "Bonebiomarkers in periodontal disease: a review article", J. Ciln. Diagn. Res, 9, pp.ZE07~ZE10 (2015)
- Hessle L, "Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization", Proc. Natl. Acad. Sci. USA, 99, pp.9445~9449 (2002)
- Buie HR, "Automatic segmentation of cortical and trabecular compartments based on a dual threshold technique for in vivo micro-CT bone analysis", Bone, 41, pp.505~515 (2007)
- Swain MV, "State of the art of Micro-CT applications in dental research", Int. J. Oral Sci, 1, pp.177~188 (2009)
