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Protective Effect of HP08-0106 on Ligature-induced Periodontitis in Rats

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초록

Periodontitis is an inflammatory disorder of the periodontium, characterized by destruction of the tooth supporting tissues including alveolar bone and mediated by various pro-inflammatory mediators. Here, we demonstrated that HP08-0106, composed of four crude drugs-Gardenia jasminoides Grandiflora, Angelica gigas Nakai, Rehmannia glutinosa, and Schizonepeta tenuifolia in a weight ratio of 2:2:1:2, perturbs inflammatory responses, osteoclast formation in LPS-induced RAW 264.7 cells and alveolar bone resorption in ligature-induced periodontitis. HP08-0106decreased the protein level of iNOS and COX2 as well as the secreted level of IL-1β, indicating that HP08-0106 has antiinflammatory effects. HP08-0106 also inhibited the expression of genes associated with osteoclastogenesis including c-Fos, MMP-9 and TRAP. Moreover, HP08-0106exhibited a protective effect from alveolar bone loss in ligature-induced periodontitis animal models. Our results strongly suggest that HP08-0106 represent an important therapeutic tool to treat inflammatory disorders associated with bone loss such as periodontitis.

Abstract

Periodontitis is an inflammatory disorder of the periodontium, characterized by destruction of the tooth supporting tissues including alveolar bone and mediated by various pro-inflammatory mediators. Here, we demonstrated that HP08-0106, composed of four crude drugs-Gardenia jasminoides Grandiflora, Angelica gigas Nakai, Rehmannia glutinosa, and Schizonepeta tenuifolia in a weight ratio of 2:2:1:2, perturbs inflammatory responses, osteoclast formation in LPS-induced RAW 264.7 cells and alveolar bone resorption in ligature-induced periodontitis. HP08-0106decreased the protein level of iNOS and COX2 as well as the secreted level of IL-1β, indicating that HP08-0106 has antiinflammatory effects. HP08-0106 also inhibited the expression of genes associated with osteoclastogenesis including c-Fos, MMP-9 and TRAP. Moreover, HP08-0106exhibited a protective effect from alveolar bone loss in ligature-induced periodontitis animal models. Our results strongly suggest that HP08-0106 represent an important therapeutic tool to treat inflammatory disorders associated with bone loss such as periodontitis.

참고문헌 (28)

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관련 논문AI추천

Protective Effect of HP08-0111 on Ligature-Induced Periodontitis소윤조, Hyoung Kwon Cho, Young Ran Park대한구강생물학회 · International Journal of Oral Biology2010 · 제35권 제4호Topical application of herbal formula for the treatment of ligature-induced periodontitis김미혜, 박정철, 양웅모, 이지혜, 이해수, 최유연대한치주과학회 · Journal of Periodontal & Implant Science2015 · 제45권 제4호Effect of Garcinia mangostana L. and propolis extracts on the inhibition of inflammation and alveolar bone loss in ligature-induced periodontitis in rats강경민, 국중기, 성세진, 유상준, 유소영, 이경현, 이대성대한구강생물학회 · International Journal of Oral Biology2019 · 제44권 제2호Inhibitory effect of pomegranate extract powder on periodontitis in rat국중기, 유상준, 이원표, Byung-Ock Kim, Joong-Hwa Kim, Kyung-Hyun Lee, Kyung-Min Kang, Se-Jin Sung, Yun-Kyong Lim치의학연구원 · Oral Biology Research2020 · 제44권 제1호Inhibitory Effects of Boesenbergia pandurata on Age-Related Periodontal Inflammation and Alveolar Bone Loss in Fischer 344 Rats김도운, 김창희, 김해봄, 정희철, 황재관한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제3호Inhibitory Effect of Standardized Curcuma xanthorrhiza Supercritical Extract on LPS-Induced Periodontitis in Rats강원구, 국교은, 김창희, 황재관한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제10호

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