Effects of Low Alternating Current on Growth of the Trichophyton tonsurans and Microsporum canis
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Background: A large number of studies have been focused on bacterial growth but limited number of literature has been reported regarding modification of fungal growth. Objective: This study aims to investigate effects of low alternating current on Microsporum (M.) canis and Trichophyton (T.) tonsurans growth. Methods: Inoculums of M. canis and T. tonsurans were applied to twenty-four PDACT (potato dextrose agar-corn meal-Tween 80) plates with a sterile spreader. Petri dishes were allocated into 8 groups according to the fungi species and the amperage delivered to these dishes. Group A, B, C and D were M. canis group and E, F, G, H were T. tonsurans group. The given amperage of electric current was 0.5 μA in group A and E, 2 μA in B and F, 4 μA in C and G. No electric current was given in group D and H. Results: In groups A, B, and C the average time elapsed for colony appearances were 42 hours, 43.17 hours, and 40.5 hours respectively. The average time elapsed in the control group D was 88.67 hours. In groups E, F, and G the average time elapsed for colony appearances were 63.67 hours, 61.83 hours, and 64.17 hours respectively. The average time elapsed in the control group H was 90.60 hours. Conclusion: With electric current, faster fungal growth was observed in the amperage range used in this study. Based on these results, we hypothesized that microcurrent helps the fungal growth.
Abstract
Background: A large number of studies have been focused on bacterial growth but limited number of literature has been reported regarding modification of fungal growth. Objective: This study aims to investigate effects of low alternating current on Microsporum (M.) canis and Trichophyton (T.) tonsurans growth. Methods: Inoculums of M. canis and T. tonsurans were applied to twenty-four PDACT (potato dextrose agar-corn meal-Tween 80) plates with a sterile spreader. Petri dishes were allocated into 8 groups according to the fungi species and the amperage delivered to these dishes. Group A, B, C and D were M. canis group and E, F, G, H were T. tonsurans group. The given amperage of electric current was 0.5 μA in group A and E, 2 μA in B and F, 4 μA in C and G. No electric current was given in group D and H. Results: In groups A, B, and C the average time elapsed for colony appearances were 42 hours, 43.17 hours, and 40.5 hours respectively. The average time elapsed in the control group D was 88.67 hours. In groups E, F, and G the average time elapsed for colony appearances were 63.67 hours, 61.83 hours, and 64.17 hours respectively. The average time elapsed in the control group H was 90.60 hours. Conclusion: With electric current, faster fungal growth was observed in the amperage range used in this study. Based on these results, we hypothesized that microcurrent helps the fungal growth.
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