Trichophyton rubrum에 대한 in vitro Terbinafine Hydrochloride, Paeonia 자연추출물, 자외선 C의 항진균 효과 비교연구
Comparative Study of In Vitro Inhibitory Effects of UVC, Terbinafine Hydrochloride 1% and Paeonia Natural Extracts on Trichophyton rubrum
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Trichophyton(T.) rubrum is most common fungal pathogen that causes tinea pedis andonychomycosis. It recurrently infects human and usually persists for very long time, provoking publichealth concern. Due to the limitation in current treatment options, alternative therapies are desirable. Weinvestigated the inhibitory effect of UVC, terbinafine hydrochloride 1% and paeonia natural extracts onT. rubrum in vitro. Objective and Method: Total 25 T. rubrum strains were cultured for 10 days on Mycosel agar plate;5 strains of T. rubrum and 5 copies for each strain. They were divided into 5 groups: control, UVCirradiation, terbinafine spray, paeonia natural extracts spray, UVC and paeonia natural extracts sprays. The cultured media were irradiated for 1 hour daily for 3 weeks in the germicidal lamp emitting 253.7nm (UVC), power of 2.875 mW/cm2 at 10 cm distance. Terbinafine and paeonia extracts was sprayedtwice on the surface to fully cover the colony area. The median diameter of each colony were measuredevery other day for 3 weeks. The change of colony diameter and the growth rate were analyzed. Results: The UVC had virtually no effect on restraining the growth of T. rubrum, similar with thegrowth of the control group. However, both the terbinafine spray and paeonia extracts slowed down thegrowth rate remarkably and showed a similar effect. Conclusion: We could only figure out the fungistatic effect, and not the fungicidal effect of paeoniaextract and terbinafine hydrochloride in vitro. UVC irradiation setting in this study was totally ineffective. More studies are needed on more variable wavelength and the fluence of UVC irradiation. In addition,further verification on the mechanism and the effect of anti-fungal activity by paeonia extracts are needed.
Abstract
Background: Trichophyton(T.) rubrum is most common fungal pathogen that causes tinea pedis andonychomycosis. It recurrently infects human and usually persists for very long time, provoking publichealth concern. Due to the limitation in current treatment options, alternative therapies are desirable. Weinvestigated the inhibitory effect of UVC, terbinafine hydrochloride 1% and paeonia natural extracts onT. rubrum in vitro. Objective and Method: Total 25 T. rubrum strains were cultured for 10 days on Mycosel agar plate;5 strains of T. rubrum and 5 copies for each strain. They were divided into 5 groups: control, UVCirradiation, terbinafine spray, paeonia natural extracts spray, UVC and paeonia natural extracts sprays. The cultured media were irradiated for 1 hour daily for 3 weeks in the germicidal lamp emitting 253.7nm (UVC), power of 2.875 mW/cm2 at 10 cm distance. Terbinafine and paeonia extracts was sprayedtwice on the surface to fully cover the colony area. The median diameter of each colony were measuredevery other day for 3 weeks. The change of colony diameter and the growth rate were analyzed. Results: The UVC had virtually no effect on restraining the growth of T. rubrum, similar with thegrowth of the control group. However, both the terbinafine spray and paeonia extracts slowed down thegrowth rate remarkably and showed a similar effect. Conclusion: We could only figure out the fungistatic effect, and not the fungicidal effect of paeoniaextract and terbinafine hydrochloride in vitro. UVC irradiation setting in this study was totally ineffective. More studies are needed on more variable wavelength and the fluence of UVC irradiation. In addition,further verification on the mechanism and the effect of anti-fungal activity by paeonia extracts are needed.
참고문헌 (17)
- Lusiana, "Muller-Goymann CC. Infected nail plate model made of human hair keratin for evaluating the efficacy of different topical antifungal formulations against Trichophyton rubrum in vitro", Erup J Pharm Biopharm, 84, pp.599~605 (2013)
- Dai T, "Ultraviolet C inactivation of dermatophytes:implications for treatment of onychomycosis", Br J Dermatol, 158, pp.1239~1246 (2008)
- Rotta I, "Efficacy of topical antifungals in the treatment of dermatophytosis", JAMA Dermatol, 49, pp.341~349 (2013)
- Smijs TGM, "The susceptibility of dermatophytes to photodynamic treatment with special focus on Trichophyton rubrum", Photochem Photobiol, 87, pp.2~13 (2011)
- Ghannoum AM, "Optimization of an Infected Shoe Model for the Evaluation of an Ultraviolet Shoe Sanitizer Device", J Am Podiat Med Asssoc, 102, pp.309~313 (2012)
- Suwadee S, "In vitro-in vivo correlation study for the dermatopharmacokinetics of terbinafine hydrochloride topical cream", Drug Development and Industrial Pharmacy, 39, pp.1372~1377 (2013)
- Carney C, "Treatment of onychomycosis using a submillisecond 1,064-nm neodymium:yttrium-aluminum-garnet laser", J Am Acad Dermatol, 69, pp.578~582 (2013)
- Hafidh RR, "Inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product", Open Microbiol J, 5, pp.96~106 (2011)
- Dai T, "Ultraviolet-C light for treatment of Candida albicans burn infection in mice", Photochem Photobiol, 87, pp.342~349 (2011)
- Begum M, "Inactivation of food spoilage fungi by ultra violet irradiation", Int J Food Microbiol, 129, pp.74~77 (2009)
- Dai T, "Ultraviolet C irradiation: an alternative antimicrobial approach to localized infections?", Expert Rev Anti Infect Ther, 10, pp.185~195 (2012)
- Dai T, "UVC light prophylaxis for cutaneous wound infections in mice", Antimicrob Agents Chemother, 56, pp.3841~3848 (2012)
- Cronin LJ, "An investigation into the inhibitory effect of ultraviolet radiation on Trichophyton rubrum", Laser Med Sci (2013)
- Yuan Y, "Functional diversity of genes for the biosynthesis of paeoniflorin and its derivatives in paeonia", Int J Mol Sci, 14, pp.18502~18519 (2013)
- Araujo MGDF, "Evaluation of Syngoanthus nitens (Bong) Ruhl extract as antifungal and in treatment of vulvovaginal candidiasis", Medl Mycol, 51, pp.673~682 (2013)
- Netkova L, "Czech ethanolfree propolis extract displays inhibitory activity against a broad spectrum of bacterial and fungal pathogens", J Food Sci, 78, pp.1421~1429 (2013)
- Askarne L, "Use of Moroccan medicinal plant extracts as botanical fungicide against citrus blue mould", Letters Appl Microbiol, 56, pp.37~43 (2012)