Participation of Central P2X7 Receptors in CFA-induced Inflammatory Pain in the Orofacial Area of Rats
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We investigated the role of central P2X receptors ininflammatory pain transmission in the orofacial area in rats. Experiments were carried out using male Sprague-Dawleyrats weighing 230-280g. Complete Freund's adjuvant (CFA,40 μL) was applied subcutaneously to the vibrissa pad toproduce inflammatory pain. The intracisternal administrationof iso-PPADS tetrasodium salt, a non-selective P2X receptorantagonist, A317491 sodium salt hydrate, a P2X2/3 receptorantagonist, 5-BDBD, a P2X4 receptor antagonist, or A438079hydrochloride, a P2X7 receptor antagonist, was performed 5days after CFA injection. Subcutaneous injections of CFAproduced increases in thermal hypersensitivity. Intracisternalinjections of iso-PPADS (25 μg) or A438079 (25 or 50 μg)produced significant anti-hyperalgesic effects against thermalstimuli compared to the vehicle group. A317491 or 5-BDBDdid not affect the head withdrawal latency times in ratsshowing an inflammatory response. Subcutaneous injectionsof CFA resulted in the up-regulation of OX-42, a microgliamarker, and GFAP, an astrocyte marker, in the medullarydorsal horn. The intracisternal administration of A438079reduced the numbers of activated microglia and astrocytes in the medullary dorsal horn. These results suggest that ablockade of the central P2X7 receptor produces antinociceptiveeffects, mediated by inhibition of glial cellfunction in the medullary dorsal horn. These data also indicatethat central P2X7 receptors are potential targets for futuretherapeutic approaches to inflammatory pain in the orofacialarea.
Abstract
We investigated the role of central P2X receptors ininflammatory pain transmission in the orofacial area in rats. Experiments were carried out using male Sprague-Dawleyrats weighing 230-280g. Complete Freund's adjuvant (CFA,40 μL) was applied subcutaneously to the vibrissa pad toproduce inflammatory pain. The intracisternal administrationof iso-PPADS tetrasodium salt, a non-selective P2X receptorantagonist, A317491 sodium salt hydrate, a P2X2/3 receptorantagonist, 5-BDBD, a P2X4 receptor antagonist, or A438079hydrochloride, a P2X7 receptor antagonist, was performed 5days after CFA injection. Subcutaneous injections of CFAproduced increases in thermal hypersensitivity. Intracisternalinjections of iso-PPADS (25 μg) or A438079 (25 or 50 μg)produced significant anti-hyperalgesic effects against thermalstimuli compared to the vehicle group. A317491 or 5-BDBDdid not affect the head withdrawal latency times in ratsshowing an inflammatory response. Subcutaneous injectionsof CFA resulted in the up-regulation of OX-42, a microgliamarker, and GFAP, an astrocyte marker, in the medullarydorsal horn. The intracisternal administration of A438079reduced the numbers of activated microglia and astrocytes in the medullary dorsal horn. These results suggest that ablockade of the central P2X7 receptor produces antinociceptiveeffects, mediated by inhibition of glial cellfunction in the medullary dorsal horn. These data also indicatethat central P2X7 receptors are potential targets for futuretherapeutic approaches to inflammatory pain in the orofacialarea.
참고문헌 (47)
- Burnstock G, "Physiology and pathophysiology of purinergic neurotransmission", Physiol Rev, 87, pp.659~797 (2007)
- Collier HO, "Antagonism by aspirin and fenamates of bronchoconstriction and nociception induced by adenosine-5'-triphosphate", Nature, 212, pp.411~412 (1966)
- Hamilton SG, "ATP in human skin elicits a dose-related pain response which is potentiated under conditions of hyperalgesia", Brain, 123, pp.1238~1246 (2000)
- Abbracchio MP, "Purinoceptors: are there families of P2X and P2Y purinoceptors?", Pharmacol Ther, 64, pp.445~475 (1994)
- Ralevic V, "Receptors for purines and pyrimidines", Pharmacol Rev, 50, pp.413~492 (1998)
- Chizh BA, "P2X receptors and nociception", Pharmacol Rev, 53, pp.553~568 (2001)
- Dunn PM, "P2X receptors in peripheral neurons", Prog Neurobiol, 65, pp.107~134 (2001)
- Inoue K, "P2 receptors and chronic pain", Purinergic Signal, 3, pp.135~144 (2007)
- Bardoni R, "ATP P2X receptors mediate fast synaptic transmission in the dorsal horn of the rat spinal cord", J Neurosci, 17, pp.5297~5304 (1997)
- Dubyak GR, "Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides", Am J Physiol, 265, pp.C577~606 (1993)
- Khakh BS, "ATP receptor-mediated enhancement of fast excitatory neurotransmitter release in the brain", Mol Pharmacol, 54, pp.372~378 (1998)
- Chen M, "A P2X receptor-mediated nociceptive afferent pathsay to lamina I of the spinal cord", Mol Pain, 1, pp.4~ (2005)
- Le KT, "Sensory presynaptic and widespread somatodendritic immunolocalization of central ionotropic P2X ATP receptors", Neuroscience, 83, pp.177~190 (1998)
- North RA, "Molecular physiology of P2X receptors", Physiol Rev, 82, pp.1013~1067 (2002)
- Burnstock G., "Purinergic receptors and pain", Curr Pharm Des, pp.1717~1735 (2009)
- Fukuhara N, "Regulation of development of allodynia by intrathecally administered P2 purinoceptor agonists and antagonists in mice", Neurosci Lett, 292, pp.25~28 (2000)
- Tsuda M, "In vivo pathway of thermal hyperalgesia by intrathecal administration of alpha, beta-methylene ATP in mouse spinal cord : involvement of the glutamate-NMDA receptor system", Br J Pharmacol, 127, pp.449~456 (1999)
- Tsuda M, "Evidence for the involvement of spinal endogenous ATP and P2X receptors in nociceptive responses caused by formalin and capsaicin in mice", Br J Pharmacol, 128, pp.1497~1504 (1999)
- Min-Kyoung Park, "Participation of Peripheral P2X Receptors in Orofacial Inflammatory Nociception in Rats", International Journal of Oral Biology, 36(3), pp.143~148 (2011), 대한구강생물학회
- Han SR, "Early dexamethasone relieves trigeminal neuropathic pain", J Dent Res, 89, pp.915~920 (2010)
- Ahn DK, "Blockade of central cyclooxygenase(COX)pathways enhances the cannabinoid-induced antinociceptive effects of inflammatory temporomandibular joint(TMJ)nociception", Pain, 132, pp.23~32 (2007)
- Kyoung A. Won, "Intracisternal Administration of Voltage Dependent Calcium Channel Blockers Attenuates Orofacial Inflammatory Nociceptive Behavior in Rats", International Journal of Oral Biology, 36(2), pp.43~50 (2011), 대한구강생물학회
- Yaksh TL, "Chronic catheterization of spinal subarachnoid space", Physiol Behav, 17, pp.1031~1036 (1976)
- Kui Y. Yang, "Glia Dose not Participate in Antinociceptive Effects of Gabapentin in Rats with Trigeminal Neuropathic Pain", International Journal of Oral Biology, 37(3), pp.121~129 (2012), 대한구강생물학회
- Surprenant A, "The cytolytic P2Z receptor for extracellular ATP identified as a P2X receptor(P2X7)", Science, 272, pp.735~738 (1996)
- Collo G, "Tissue distribution of the P2X receptor", Neuropharmacology, 36, pp.1277~1283 (1997)
- Yu Y, "Cellular localization of P2X7 receptor mRNA in the rat brain", Brain Res, 1194, pp.45~55 (2008)
- Franke H, "P2X receptor expression on astrocytes in the nucleus accumbens of rats", Neuroscience, 108, pp.421~429 (2001)
- Chessell IP, "Disruption of the P2X7 purinoceptor gene abolishes chronic inflammatory and neuropathic pain", Pain, 114, pp.386~396 (2005)
- Zhou ZH, "Contribution of the spinal P2X7 receptors to bee venom-induced nociception and inflammation in conscious rats", Neurosci Lett, 531, pp.145~148 (2012)
- Murasaki K, "P2X7 receptor and cytokines contribute to extra-territorial facial pain", J Dent Res, 92, pp.260~265 (2013)
- Itoh K, "Central sensitization of nociceptive neurons in rat medullary dorsal horn involves purinergic P2X7 receptors", Neuroscience, 192, pp.721~731 (2011)
- McGaraughty S, "Effects of A-317491, a novel and selective P2X3", P2X2, Br J Pharmacol, pp.2003, 140[DOI]
- Guo LH, "Expression of P2X4 receptor by lesional activated microglia during formalin-induced inflammatory pain", J Neuroimmunol, 163, pp.120~127 (2005)
- Tsuda M, "P2X4 receptor induced in spinal microglia gate tactile allodynia after nerve injury", Nature, 424, pp.778~783 (2003)
- Tsuda M, "Behavioral phenotypes of mice lacking purinergic P2X4 receptors in acute and chronic pain assays", Mol Pain, 5, pp.28~ (2009)
- Hu B, "P2X receptors in trigeminal subnucleus caudalis modulate central sensitization in trigeminal subnucleus oralis", J Neurophysiol, 88, pp.1614~1624 (2002)
- Hui J, "Repetitive hyperbaric oxygen treatment attenuates complete Freund's adjuvant-induced pain and reduces glia-mediated neuroinflammation in the spinal cord", J Pain, 14, pp.747~758 (2013)
- Raghavendra V, "Complete Freunds adjuvant-induced peripheral inflammation evokes glial activation and proinflammatory cytokine expression in the CNS", Eur J Neurosci, 20, pp.467~473 (2004)
- Cao H, "Spinal glial activation contributes to pathological pain states", Neurosci Biobehav Rev, 32, pp.972~983 (2008)
- Kreutzberg GW, "Microglia : a sensor for pathological events in the CNS", Trends Neurosci, 19, pp.312~318 (1996)
- Dong Y, "Immune function of astrocytes", Glia, 36, pp.180~190 (2001)
- Milligan ED, "Pathological and protective roles of glia in chronic pain", Nat Rev Neurosci, 10, pp.23~36 (2009)
- Watkins LR, "Glia : a novel drug discovery target for clinical pain", Nat Rev Drug Discov, 2, pp.973~985 (2003)
- Miller RJ, , Handb Exp Pharmacol, 194, pp.417~449 (2009)
- Saab CY, "Alarm curse? The pain of neuroinflammation", Brain Res Rev, 58, pp.226~235 (2008)
- Surprenant A, "Singaling at purinergic P2X receptors", Annu Rev Physiol, 71, pp.333~359 (2009)