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Participation of Peripheral P2X Receptors in Orofacial Inflammatory Nociception in Rats

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초록

The present study investigated the role of peripheral P2X receptors in inflammatory pain transmission in the orofacial area in rats. Experiments were carried out on male Sprague-Dawley rats weighing 220 to 280 g. Formalin (5%, 50 μL) and complete Freund's adjuvant (CFA, 25 μL) was applied subcutaneously to the vibrissa pad to produce inflammatory pain. TNP-ATP, a P2X_(2,2/3,4) receptor antagonist, or OX-ATP, a P2X7 receptor antagonist, was then injected subcutaneously at 20 minutes prior to formalin injection. One of the antagonists was administered subcutaneously at three days after CFA injection. The subcutaneous injection of formalin produced a biphasic nociceptive behavioral response. Subcutaneous pretreatment with TNP-ATP (80, 160 or 240 μg)significantly suppressed the number of scratches in the second phase produced by formalin injection. The subcutaneous injection of 50 μg of OX-ATP also produced significant antinociceptive effects in the second phase. Subcutaneous injections of CFA produced increases in mechanical and thermal hypersensitivity. Both TNP-ATP (480 μg) and OX-ATP (100 μg) produced an attenuation of mechanical hypersensitivity. However, no change was observed in thermal hypersensitivity after the injection of either chemical. These results suggest that the blockade of peripheral P2X receptors is a potential therapeutic approach to the onset of inflammatory pain in the orofacial area.

Abstract

The present study investigated the role of peripheral P2X receptors in inflammatory pain transmission in the orofacial area in rats. Experiments were carried out on male Sprague-Dawley rats weighing 220 to 280 g. Formalin (5%, 50 μL) and complete Freund's adjuvant (CFA, 25 μL) was applied subcutaneously to the vibrissa pad to produce inflammatory pain. TNP-ATP, a P2X_(2,2/3,4) receptor antagonist, or OX-ATP, a P2X7 receptor antagonist, was then injected subcutaneously at 20 minutes prior to formalin injection. One of the antagonists was administered subcutaneously at three days after CFA injection. The subcutaneous injection of formalin produced a biphasic nociceptive behavioral response. Subcutaneous pretreatment with TNP-ATP (80, 160 or 240 μg)significantly suppressed the number of scratches in the second phase produced by formalin injection. The subcutaneous injection of 50 μg of OX-ATP also produced significant antinociceptive effects in the second phase. Subcutaneous injections of CFA produced increases in mechanical and thermal hypersensitivity. Both TNP-ATP (480 μg) and OX-ATP (100 μg) produced an attenuation of mechanical hypersensitivity. However, no change was observed in thermal hypersensitivity after the injection of either chemical. These results suggest that the blockade of peripheral P2X receptors is a potential therapeutic approach to the onset of inflammatory pain in the orofacial area.

참고문헌 (42)

  1. Abbracchio MP, "Purinergic signalling: pathophysiological roles", Jpn J Pharmacol, 78, pp.113~145 (1998)
  2. Ahn DK, "Central cyclooxygenase inhibitors reduced IL-1beta-induced hyperalgesia in temporomandibular joint of freely moving rats", Pain, 117, pp.204~213 (2005)
  3. Alavi AM, "Immunohistochemical evidence for ATP receptors in human dental pulp", J Dent Res, 80, pp.476~483 (2001)
  4. Burnstock G, "Evidence that adenosine triphosphate or a related nucleotide is the transmitter substance released by non-adrenergic inhibitory nerves in the gut", Br J Pharmacol, 40, pp.668~688 (1970)
  5. Burnstock G, "Cellular distribution and functions of P2 receptor different systems", Int Rev Cytol, 240, pp.31~304 (2004)
  6. Burnstock G, "Physiology and pathophysiology of purinergic neurotransmission", Physiol Rev, 87, pp.659~797 (2007)
  7. Cairns BE, "Evidence that excitatory amino acid receptors within the temporomandibular joint region are involved in the reflex activation of the jaw muscles", J Neurosci, 18, pp.8056~8064 (1998)
  8. Chen Y, "Activation of P2X7 receptors in glial satellite cells reduces pain through downregulation of P2X3 receptors in nociceptive neurons", Proc Natl Acad Sci USA, 105, pp.16773~16778 (2008)
  9. Chessell IP, "Disruption of the P2X7 purinoceptor gene abolishes chronic inflammatory and neuropathic pain", Pain, 114, pp.386~396 (2005)
  10. Choi HS, "Central cyclooxygenase-2 participates in interleukin-1 beta-induced hyperalgesia in the orofacial formalin test of freely moving rats", Neurosci Lett, 352, pp.187~190 (2003)
  11. Choi HS, "Effects of intracisternal injection of interleukin-6 on nociceptive jaw opening reflex and orofacial formalin test in freely moving rats", Brain Res Bull, 59, pp.365~370 (2003)
  12. Choi HS, "Central cyclooxygenase-2 participates in interleukin-1 beta-induced hyperalgesia in the orofacial formalin test of freely moving rats", Neurosci Lett, 352, pp.187~190 (2003)
  13. Clavelou P, "Application of the formalin test to the study of orofacial pain in the rat", Neurosci Lett, 103, pp.349~353 (1989)
  14. Collier HO, "Antagonism by aspirin and fenamates of bronchoconstriction and nociception induced by adenosine-5'- triphosphate", Nature, 212, pp.411~412 (1966)
  15. Dowd E, "P2X receptor-mediated excitation of nociceptive afferents in the normal and arthritic rat knee joint", Br J Pharmacol, 125, pp.341~346 (1998)
  16. Drury AN, "The physiological activity of adenine compounds with especial reference to their action upon the mammalian heart", J Physiol, 68, pp.213~237 (1929)
  17. Holton P, "The liberation of adenosine triphosphate on antidromic stimulation of sensory nerves", J Physiol, 145, pp.494~504 (1959)
  18. Honore P, "The antihyperalgesic activity of a selective P2X7 receptor antagonist, A-839977, is lost in IL-1alphabeta knockout mice", Behav Brain Res, 204, pp.77~81 (2009)
  19. Ichikawa H, "VR1-, VRL-1- and P2X3 receptor-immunoreactive innervation of the rat temporomandibular joint", Brain Res, 1008, pp.131~136 (2004)
  20. Iwata K, "Medullary dorsal horn neuronal activity in rats with persistent temporomandibular joint and perioral inflammation", J Neurophysiol, 82, pp.1244~1253 (1999)
  21. Jarvis MF, "A-317491, a novel potent and selective non-nucleotide antagonist of P2X3 and P2X2", 3 receptors, reduces chronic inflammatory and neuropathic pain in the rat, 2002, pp.99, 17179~17184
  22. Jung CY, "Participation of peripheral group I and II metabotropic glutamate receptors in the development or maintenance of IL-1 beta-induced mechanical allodynia in the orofacial area of conscious rats", NEUROSCIENCE LETTERS, 409, pp.173~178, ELSEVIER IRELAND LTD
  23. 김상희, "Purinergic Receptors Play Roles in Secretion of Rat von Ebner Salivary Gland", International Journal of Oral Biology, 31(4), pp.141~148 (2006), 대한구강생물학회
  24. Kim YS, "Expression of P2X3 receptor in the trigeminal sensory nuclei of the rat", J Comp Neurol, 506, pp.627~639 (2008)
  25. Lee HJ, "Intracisternal NMDA produces analgesia in the orofacial formalin test of freely moving rats", Prog Neuropsychopharmacol Biol Psychiatry, 28, pp.497~503 (2004)
  26. Lee S, "Distinctive response of CNS glial cells in oro-facial pain associated with injury, infection and inflammation", Mol Pain, 6, pp.79~ (2010)
  27. Hai Ying Li, "Pharmacological and electrophysiological characterization of rat P2X currents", International Journal of Oral Biology, 33(1), pp.1~5 (2008), 대한구강생물학회
  28. McGaraughty S, "Effects of A-317491, a novel and selective P2X3", P2X2, Br J Pharmacol, pp.2003, 140[DOI]
  29. Nörenberg W, "Neuronal P2X receptors: localisation and functional properties", Naunyn Schmiedebergs Arch Pharmacol, 362, pp.324~339 (2000)
  30. Oliveira MC, "Peripheral mechanisms underlying the essential role of P2X3,2", 3 receptors in the development of inflammatory hyperalgesia, 2009, pp.141, 127~134
  31. Ren K, "An inflammtion", hyperalgesia model for the study of orofacial pain, 1996, pp.75, 217~
  32. Seo HS, "Acidic pH facilitates peripheral alpha beta meATP-mediated nociception in rats: Differential roles of P2X, P2Y, ASIC and TRPV1 receptors in ATP-induced mechanical allodynia and thermal hyperalgesia", NEUROPHARMACOLOGY, 60(4), pp.580~586, PERGAMON-ELSEVIER SCIENCE LTD
  33. Shaver SR, "P2Y receptors: biological advances and therapeutic opportunities", Curr Opin Drug Discov Devel, 4, pp.665~670 (2001)
  34. Shinoda M, "Changes in P2X3 receptor expression in the trigeminal ganglion following monoarthritis of the temporomandibular joint in rats", Pain, 116, pp.42~51 (2005)
  35. Staikopoulos V, "Localization of P2X2 and P2X3 receptors in rat trigeminal ganglion neurons", Neuroscience, 144, pp.208~216 (2007)
  36. Tashiro A, "Differential effects of estradiol on encoding properties of TMJ units in laminae I and V at the spinomedullary junction in female rats", J Neurophysiol, 98, pp.3242~3253 (2007)
  37. Teixeira JM, "Pelegrini-da-Silva A, Parada CA, Tambeli CH. Involvement of temporomandibular joint P2X3 and P2X2", 3 receptors in carrageenan-induced inflammatory hyperalgesia in rats, 2010, pp.645, 79~85
  38. Tjolsen A, "The formalin test: an evaluation of the method", Pain, 51, pp.5~17 (1992)
  39. Volonté C, "Extracellular ATP and neurodegeneration", Curr Drug Targets CNS Neurol Disord, 2, pp.403~412 (2003)
  40. Yang GY, "Participation of NMDA and non NMDA glutamate receptors in the formalin-induced inflammatory temporomandibular joint noception", Interantional Journal of Oral Biology, 32, pp.59~65 (2007)
  41. Zhou Q, "Persistent Fos protein expression after orofacial deep or cutaneous tissue inflammation in rats: implications for persistent orofacial pain", J Comp Neurol, 412, pp.276~291 (1999)
  42. Ziganshin AU, "P2 receptors: theoretical background for the use in clinical practice", Bull Exp Biol Med, 134, pp.313~317 (2002)

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