Effect of hypoxia on angiogenesis-related proteins in human dental pulp cells
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초록
Dental pulp is a highly vascularized tissue with high regenerative potential. Revascularization of severed vasculature in the tooth is required for pulp healing during avulsed tooth treatment. In this study, the relative expression of angiogenesis-related proteins was determined in human dental pulp cells using a human angiogenesis proteome profiler array. The proteome profiler array detected differentially expressed angiogenesis-related factors under conditions of hypoxia, which enhances the angiogenic potential of dental pulp cells. We confirmed that hypoxia regulates the mRNA expression of angiogenesis-related factors, including CXCL16 in dental pulp cells. Furthermore, conditioned media of hypoxic pulp cells induced tube-like structures of vascular endothelial cells, which were reduced by the neutralization of CXCL16 function. In conclusion, our data show that angiogenesisrelated factors are differentially expressed by hypoxia in dental pulp cells and suggest that CXCL16 may involve in the revascularization of hypoxic dental pulp.
Abstract
Dental pulp is a highly vascularized tissue with high regenerative potential. Revascularization of severed vasculature in the tooth is required for pulp healing during avulsed tooth treatment. In this study, the relative expression of angiogenesis-related proteins was determined in human dental pulp cells using a human angiogenesis proteome profiler array. The proteome profiler array detected differentially expressed angiogenesis-related factors under conditions of hypoxia, which enhances the angiogenic potential of dental pulp cells. We confirmed that hypoxia regulates the mRNA expression of angiogenesis-related factors, including CXCL16 in dental pulp cells. Furthermore, conditioned media of hypoxic pulp cells induced tube-like structures of vascular endothelial cells, which were reduced by the neutralization of CXCL16 function. In conclusion, our data show that angiogenesisrelated factors are differentially expressed by hypoxia in dental pulp cells and suggest that CXCL16 may involve in the revascularization of hypoxic dental pulp.
참고문헌 (30)
- Abd-Elmeguid A, "Dental pulp neurophysiology: Part 1. clinical and diagnostic implications", J Can Dent Assoc, 75, pp.55~59 (2009)
- Yoon-Kyu Park, "A Trial of Screening of Genes Involved in Odontoblasts Differentiation from Human Dental Pulp Stem Cells", International Journal of Oral Biology, 37(4), pp.167~173 (2012), 대한구강생물학회[DOI]
- M. Nakashima, "Regeneration of Dental Pulp by Stem Cells", Advances in Dental Research, 23(3), pp.313~319 (2011), SAGE Publications[DOI]
- L. Tran-Hung, "Role of Human Pulp Fibroblasts in Angiogenesis", Journal of Dental Research, 85(9), pp.819~823 (2006), SAGE Publications[DOI]
- Peter Carmeliet, "Angiogenesis in cancer and other diseases", Nature, 407(6801), pp.249~257 (2000), Springer Nature[DOI]
- L ARTESE, "Vascular Endothelial Growth Factor (VEGF) Expression in Healthy and Inflamed Human Dental Pulps", Journal of Endodontics, 28(1), pp.20~23 (2002), Ovid Technologies (Wolters Kluwer Health)[DOI]
- E.M. Mullane, "Effects of VEGF and FGF2 on the Revascularization of Severed Human Dental Pulps", Journal of Dental Research, 87(12), pp.1144~1148 (2008), SAGE Publications[DOI]
- Gabriele Bergers, "Angiogenesis: Tumorigenesis and the angiogenic switch", Nature Reviews Cancer, 3(6), pp.401~410 (2003), Springer Nature[DOI]
- Lifen Li, "Hypoxia Promotes Mineralization of Human Dental Pulp Cells", Journal of Endodontics, 37(6), pp.799~802 (2011), Elsevier BV[DOI]
- Y. Fukuyama, "Hypoxia Induces Expression and Activation of AMPK in Rat Dental Pulp Cells", Journal of Dental Research, 86(9), pp.903~907 (2007), SAGE Publications[DOI]
- K. Ito, "Hypoxic condition promotes differentiation and mineralization of dental pulp cells in vivo", International Endodontic Journal, 48(2), pp.115~123 (2015), Wiley-Blackwell[DOI]
- Jaruma Beau Sakdee, "Hypoxia-amplified Proliferation of Human Dental Pulp Cells", Journal of Endodontics, 35(6), pp.818~823 (2009), Elsevier BV[DOI]
- Andreza M.F. Aranha, "Hypoxia Enhances the Angiogenic Potential of Human Dental Pulp Cells", Journal of Endodontics, 36(10), pp.1633~1637 (2010), Elsevier BV[DOI]
- Masae Kitagawa, "Immortalization and characterization of human dental pulp cells with odontoblastic differentiation", Archives of Oral Biology, 52(8), pp.727~731 (2007), Elsevier BV[DOI]
- Gregg L. Semenza, "Hypoxia-Inducible Factors in Physiology and Medicine", Cell, 148(3), pp.399~408 (2012), Elsevier BV[DOI]
- Terhi Jokilehto, "The role of HIF prolyl hydroxylases in tumour growth", Journal of Cellular and Molecular Medicine, 14(4), pp.758~770 (2010), Wiley-Blackwell[DOI]
- Kim S, "Effects of selected inflammatory mediators on blood flow and vascular permeability in the dental pulp", Proc Finn Dent Soc, 88(1), pp.387~392 (1992)
- L. Tran-Hung, "Quantification of angiogenic growth factors released by human dental cells after injury", Archives of Oral Biology, 53(1), pp.9~13 (2008), Elsevier BV[DOI]
- Kajdaniuk D, "Vascular endothelial growth factor (VEGF) - part 1: In physiology and pathophysiology", Endokrynol Pol, 62, pp.444~455 (2011)
- S GONCALVES, "Tooth Slice–Based Models for the Study of Human Dental Pulp Angiogenesis", Journal of Endodontics, 33(7), pp.811~814 (2007), Elsevier BV[DOI]
- Maria G. Mantellini, "Adhesive resin and the hydrophilic monomer HEMA induce VEGF expression on dental pulp cells and macrophages", Dental Materials, 22(5), pp.434~440 (2006), Elsevier BV[DOI]
- Sylvie Mathieu, "Role of injured endothelial cells in the recruitment of human pulp cells", Archives of Oral Biology, 50(2), pp.109~113 (2005), Elsevier BV[DOI]
- Michel Goldberg, "Cells and extracellular matrices of dentin and pulp: A biological basis for repair and tissue engineering", Critical Reviews in Oral Biology & Medicine, 15(1), pp.13~27 (2004), SAGE Publications[DOI]
- Jessica A. Bertout, "The impact of O2 availability on human cancer", Nature Reviews Cancer, 8(12), pp.967~975 (2008), Springer Nature[DOI]
- Warren S. Pear, "Lasting longer without oxygen: The influence of hypoxia on Notch signaling", Cancer Cell, 8(6), pp.435~437 (2005), Elsevier BV[DOI]
- Ling Deng, "CXCR6", CXCL16 functions as a regulator in metastasis and progression of cancer, 2010, pp.1806(1), 42~49[DOI]
- Mehrdad Matloubian, "A transmembrane CXC chemokine is a ligand for HIVcoreceptor bonzo", Nature Immunology, 1(4), pp.298~304 (2000), Springer Nature[DOI]
- T. Shimaoka, "Molecular Cloning of a Novel Scavenger Receptor for Oxidized Low Density Lipoprotein, SR-PSOX, on Macrophages", Journal of Biological Chemistry, 275(52), pp.40663~40666 (2000), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
- Franklin H. Epstein, "Chemokines — Chemotactic Cytokines That Mediate Inflammation", New England Journal of Medicine, 338(7), pp.436~445 (1998), New England Journal of Medicine (NEJM[DOI]
- Xin Zhuge, "CXCL16 is a novel angiogenic factor for human umbilical vein endothelial cells", Biochemical and Biophysical Research Communications, 331(4), pp.1295~1300 (2005), Elsevier BV[DOI]