Differentiation of CD31-Positive Vascular Endothelial Cells from Organoid Culture of Dental Pulp Stem Cells
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.
Abstract
The mesenchymal stem cells (MSCs) that reside in dental tissues hold a great potential for future applications in regenerative dentistry. In this study, we used human dental pulp cells, isolated from the molars (DPCs), in order to establish the organoid culture. DPCs were established after growing pulp cells in an MSC expansion media (MSC-EM). DPCs were subjected to organoid growth media (OGM) in comparison with human dental pulp stem cells (DPSCs). Inside the extracellular matrix in the OGM, the DPCs and DPSCs readily formed vessel-like structures, which were not observed in the MSC-EM. Immunocytochemistry analysis and flow cytometry analysis showed the elevated expression of CD31 in the DPCs and DPSCs cultured in the OGM. These results suggest endothelial cell-prone differentiation of the DPCs and DPSCs in organoid culture condition.
참고문헌 (19)
- Pittenger MF, "Multilineage potential of adult human mesenchymal stem cells", Science, 284, pp.143~147 (1999)[DOI]
- Nombela-Arrieta C, "The elusive nature and function of mesenchymal stem cells", Nat Rev Mol Cell Biol, 12, pp.126~131 (2011)[DOI]
- Sharpe PT, "Dental mesenchymal stem cells", Development, 143, pp.2273~2280 (2016)[DOI]
- Liu J, "Concise reviews: Characteristics and potential applications of human dental tissue-derived mesenchymal stem cells", Stem Cells, 33, pp.627~638 (2015)[DOI]
- Zhao H, "Secretion of shh by a neurovascular bundle niche supports mesenchymal stem cell homeostasis in the adult mouse incisor", Cell Stem Cell, 14, pp.160~173 (2014)[DOI]
- Neves VC, "Promotion of natural tooth repair by small molecule GSK3 antagonists", Sci Rep, 7, pp.39654~ (2017)[DOI]
- Babb R, "Axin2-expressing cells differentiate into reparative odontoblasts via autocrine Wnt", beta-catenin signaling in response to tooth damage, 2017, pp.7, 3102~
- Kaukua N, "Glial origin of mesenchymal stem cells in a tooth model system", Nature, 513, pp.551~554 (2014)[DOI]
- Huch M, "Modeling mouse and human development using organoid cultures", Development, 142, pp.3113~3125 (2015)[DOI]
- Fatehullah A, "Organoids as an in vitro model of human development and disease", Nat Cell Biol, 18, pp.246~254 (2016)[DOI]
- Clevers H, "Modeling Development and Disease with Organoids", Cell, 165, pp.1586~1597 (2016)[DOI]
- Lancaster MA, "Organogenesis in a dish: modeling development and disease using organoid technologies", Science, 345, pp.1247125~ (2014)[DOI]
- Ogawa M, "Functional salivary gland regeneration by transplantation of a bioengineered organ germ", Nat Commun, 4, pp.2498~ (2013)[DOI]
- Maimets M, "Long-Term In Vitro Expansion of Salivary Gland Stem Cells Driven by Wnt Signals", Stem Cell Reports, 6, pp.150~162 (2016)[DOI]
- Arnaoutova I, "In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract", Nat Protoc, 5, pp.628~635 (2010)[DOI]
- Clevers H, "Defining Adult Stem Cells by Function, Not by Phenotype", Annu Rev Biochem (2018)
- Luo L, "Potential Roles of Dental Pulp Stem Cells in Neural Regeneration and Repair", Stem Cells Int, 2018, pp.1731289~ (2018)
- Sato T, "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche", Nature, 459, pp.262~265 (2009)[DOI]
- Rossant J, "Signaling pathways in vascular development", Annu Rev Cell Dev Biol, 18, pp.541~573 (2002)[DOI]