사람치수줄기세포 조건배지와 법랑모세포 조건 배지가사람치수줄기세포의 상아모세포 분화에 미치는 영향
The effects of conditioned media obtained from human dental pulp stem cells and ameloblasts on odontogenic differentiation of human dental pulp stem cells
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The differentiation ability of denta1 pu1p stem cells(DPSCs) is clinically very usefu1 for the regeneration of dentin-pu1p comp1ex. It is important to confirm that dentin-pu1p comp1ex is made when DPSCs differentiate with conditioned media(CM) inc1uding the signa1 mo1ecu1es such as various enzymes. growth factors , hormones and so on. We can identify the odontogenic differentiation of DPSCs using an odontogenic marker such as dentin sia1ophosphoprotein(DSPP) mRNA. Because the ro1e of odontob1asts is very important to deve10p dentin-pu1p comp1ex. This research is going to confirm whether treatments of condi tioned media (CM) facili tate odontob1asts differentiation of human DPSCs in vitro. When human denta1 pu1p stem cell CM (hDPSC-CM) , ame1ob1ast CM(AM-CM) , the mixture of AM-CM and hDPSC-CM are treated to hDPSCs. we examine the capacity of odontogenic differentiation of hDPSCs. Human DPSCs was exp1anted from human third mo1ars . We cu1tured to differentiate hDPSCs with hDPSC-CM. AM-CM. the mixture of hDPSC-CM and AM-CM. Human DPSCs cu1tured without CM were contro1. They were divided into 4 groups. We differentiated hDPSCs for 0, 3, 7, 10 days and operated rea1 time PCR using DSPP mRNA promoter as an odontogenic marker and BSP mRNA promoter as an osteogenic marker. Expression of DSPP mRNA on differentiation of hDPSCs with the mixture of hDPSC-CM and AM-CM increased than control after 3. 10 days and increased than on differentia tion of hDPSCs with hDPSC-CM. AM-CM respectively. Expression of BSP mRNA increased than control after 3 days but decreased after 7. 10 days. Dentin formation is important meaning on pulp regen eration . When pulp exposes stimuli . pulp develops reparative dentin. which means a sort of regeneration . Conclusively. the mixture of hDPSC-CM and AM-CM improves the ability of odontogenic differ entiation of hDPSCs and helpful to regenerate pulp.
Abstract
The differentiation ability of denta1 pu1p stem cells(DPSCs) is clinically very usefu1 for the regeneration of dentin-pu1p comp1ex. It is important to confirm that dentin-pu1p comp1ex is made when DPSCs differentiate with conditioned media(CM) inc1uding the signa1 mo1ecu1es such as various enzymes. growth factors , hormones and so on. We can identify the odontogenic differentiation of DPSCs using an odontogenic marker such as dentin sia1ophosphoprotein(DSPP) mRNA. Because the ro1e of odontob1asts is very important to deve10p dentin-pu1p comp1ex. This research is going to confirm whether treatments of condi tioned media (CM) facili tate odontob1asts differentiation of human DPSCs in vitro. When human denta1 pu1p stem cell CM (hDPSC-CM) , ame1ob1ast CM(AM-CM) , the mixture of AM-CM and hDPSC-CM are treated to hDPSCs. we examine the capacity of odontogenic differentiation of hDPSCs. Human DPSCs was exp1anted from human third mo1ars . We cu1tured to differentiate hDPSCs with hDPSC-CM. AM-CM. the mixture of hDPSC-CM and AM-CM. Human DPSCs cu1tured without CM were contro1. They were divided into 4 groups. We differentiated hDPSCs for 0, 3, 7, 10 days and operated rea1 time PCR using DSPP mRNA promoter as an odontogenic marker and BSP mRNA promoter as an osteogenic marker. Expression of DSPP mRNA on differentiation of hDPSCs with the mixture of hDPSC-CM and AM-CM increased than control after 3. 10 days and increased than on differentia tion of hDPSCs with hDPSC-CM. AM-CM respectively. Expression of BSP mRNA increased than control after 3 days but decreased after 7. 10 days. Dentin formation is important meaning on pulp regen eration . When pulp exposes stimuli . pulp develops reparative dentin. which means a sort of regeneration . Conclusively. the mixture of hDPSC-CM and AM-CM improves the ability of odontogenic differ entiation of hDPSCs and helpful to regenerate pulp.
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