Repeated batch culture of Rhizopus arrhizus RA 128 using broken rice for L (+)-lactic acid production
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The objective of this research was to study L (+)-lactic acid production byrepeated batch culture of Rhizopus arrhizus RA 128 using broken rice as sole carbonsource. The L (+)-lactic acid production increased with the increase of broken riceconcentration by 60g/L. L (+)-lactic acid production was increased from 10.9 to 25.3g/Lwhen pH was increased from 4.0 to 6.0. However, over 7.0 of pH, it was decreased. TheL (+)-lactic acid productivity and cell concentration increased with the increase intemperature from 29 to 35 ℃. The maximum L (+)-lactic acid production and cellconcentration was found with the 1.5% of CaCO3. Repeated batch culture was successfullyperformed for 4cycles for 12 days. All cycles showed that 234.5g/L of broken rice wasconsumed. 75g/L of total L (+)-lactic acid was obtained after 288hr. These results showthat broken rice can be useful for the effective production of L (+)-lactic acid in therepeated batch culture of R. arrhizus RA 128.
Abstract
The objective of this research was to study L (+)-lactic acid production byrepeated batch culture of Rhizopus arrhizus RA 128 using broken rice as sole carbonsource. The L (+)-lactic acid production increased with the increase of broken riceconcentration by 60g/L. L (+)-lactic acid production was increased from 10.9 to 25.3g/Lwhen pH was increased from 4.0 to 6.0. However, over 7.0 of pH, it was decreased. TheL (+)-lactic acid productivity and cell concentration increased with the increase intemperature from 29 to 35 ℃. The maximum L (+)-lactic acid production and cellconcentration was found with the 1.5% of CaCO3. Repeated batch culture was successfullyperformed for 4cycles for 12 days. All cycles showed that 234.5g/L of broken rice wasconsumed. 75g/L of total L (+)-lactic acid was obtained after 288hr. These results showthat broken rice can be useful for the effective production of L (+)-lactic acid in therepeated batch culture of R. arrhizus RA 128.
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