Pretreatment of Sugarcane Molasses and Citric Acid Production by Candida zeylanoides
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Citric acid is produced via submerged fermentation using yeasts. Among eight different strains of yeast, Candida zeylanoides was chosen as the strain for producing citric acid and optimized for various C/N ratios and effects of phosphate or Fe2+ ions in a clean carbon source medium (glucose: fructose, 1:1). The yield of citric acid was maximized at a C/N ratio of 40/1, a phosphate addition of 1.0 g/l, and an Fe2+ ion concentration of less than 50 mg/l, yielding up to 91 g/L in the broth with 18.5 g/l of isocitric acid in a six-day fermentation period using a pre-treated molasses medium. The yield of batch culture was 0.51 (Yp/s, g/g) in a 5 L-Jar fermenter.
Abstract
Citric acid is produced via submerged fermentation using yeasts. Among eight different strains of yeast, Candida zeylanoides was chosen as the strain for producing citric acid and optimized for various C/N ratios and effects of phosphate or Fe2+ ions in a clean carbon source medium (glucose: fructose, 1:1). The yield of citric acid was maximized at a C/N ratio of 40/1, a phosphate addition of 1.0 g/l, and an Fe2+ ion concentration of less than 50 mg/l, yielding up to 91 g/L in the broth with 18.5 g/l of isocitric acid in a six-day fermentation period using a pre-treated molasses medium. The yield of batch culture was 0.51 (Yp/s, g/g) in a 5 L-Jar fermenter.
참고문헌 (23)
- Akiyama S, "Induction and citric acid productivity of fluoroacetate-sensitive mutant strains of Candida lipolytica", Agric. Biol. Chem, 37, pp.879~884 (1973)
- Crolla A, "Optimization of citric acid production from Candida lipolytica Y-1095 using n-paraffin", J. Biotechnol, 89, pp.27~40 (2001)
- Crueger W, "Organic acids", pp.134~142 (1989), Biotechnology
- Ermakova IT, "Properties of Candida lipolytica mutants with the modified glyoxylate cycle and their ability to produce citric acid and isocitric acid", Appl. Microbiol. Biotechnol, 23, pp.372~377 (1986)
- Haq I, "Citric acid production by selected mutant of Aspergillus niger from cane molasses", Bioresource Technol, 93, pp.125~130 (2004)
- Hossain M, "Galactose inhibition of citric acid production from glucose by Aspergillus niger", Appl. Microbiol. Biotechnol, 22, pp.98~102 (1985)
- Kubicek CP, "Citric acid fermentation", CRC Crit. Rev. Biotechnol, 3, pp.331~373 (1986)
- Liu X, "Citric acid production from hydrolysate of pretreated straw cellulose by Yarrowia lipolytica SWJ-1b using batch and fed-batch cultivation", Preparative Biochem. Biotechnol, 45, pp.825~835 (2015)
- Majumder L, "Citric acid production by Aspergillus niger using molasses and pumpkin as substrate", Eur. J. Biological Sci, 2, pp.1~08 (2010)
- Marier JR, "Direct determination of citric acid in milk with an improved pyridine acetic anhydride method", J. Dairy Sci, 41, pp.1683~1692 (1958)
- Milsom PE, "Citric acid", pp.665~680 (1985), Comprehensive Biotechnology Moo-young M
- Mourya S, "Production of citric acid from starch-hydrolysate by Aspergillus niger", Microbiol. Res, 155, pp.37~44 (2000)
- Nehad ZA., "Attempts at improving citric acid fermentation by Aspergillus niger in beet-molasses medium", Bioresource Technol, 84, pp.97~100 (2002)
- Okoshi H, "Citric acid production by Candida tropicalis under high dissolved oxygen concentrations", Agric. Biol. Chem, 51, pp.257~258 (1987)
- Rane KD, "Production of citric acid by Candida lipolytica Y-1095: effect of glucose concentration on yield and productivity", Enzyme Microbiol. Technol, 15, pp.646~651 (1993)
- Rohr M, "Citric acid. in Biotechnology ed. by Rehm HJ and Reed G", 3, pp.419~460 (1983)
- Soccol RC, "New perspectives for citric acid production and application", Food Technol. Biotechnol, 44, pp.141~149 (2006)
- Shah ND, "Starch hydrolysate, an optimal and economical source of carbon for the secretion of citric acid by Yarrow lipolytica", Starch, 45, pp.104~109 (1993)
- Tani Y, "Production of citric acid from methanol by a flouroacetate-resistant mutant of Candida sp.Y-1", Appl. Microbiol. Biotechnol, 34, pp.5~9 (1990)
- Walid AL, "acid production by a novel Aspergillus niger isolate: I. Mutagenesis and cost reduction studies", Bioresource Technol, 94, pp.3464~3469 (2007)
- Umar F, "Citric acid production from sugarcane molasses by Aspergillus niger under different fermentation conditions and substrate levels", Int. J. Agric. Appl. Sci, 5, pp.8~16 (2013)
- Seda Karasu Yalcin, "Enhancing Citric Acid Production of Yarrowia lipolytica by Mutagenesis and Using Natural Media Containing Carrot Juice and Celery Byproducts", Food Science and Biotechnology, 21(3), pp.867~874 (2012), 한국식품과학회[DOI]
- Citric acid production by yeasts: Fermentation conditions, process optimization and strain improvement, "Yalcin SK, Bozdemir MT, Ozbas ZY", Cur. Res. Technol. Education Topics in Applied Microbiol. Microbial Biotechnol, 1374~1382 (2010)