Mycelium and Exopolysaccharide Production from Submerged Liquid Fermentation of Pleurotus Sajor-Caju PSC 14 using Waste Vegetable Oil as Carbon Source
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The object of this research is to study effect of the waste vegetable oil on mycelium and exopolysaccharide production from Pleurotus sajor-caju PSC 14. Among various culture temperatures, the mycelium and exopolysaccharide production were highest at 29°C. However, they decreased over and below 29°C. When waste vegetable oil 2.0g/L was used for 5 days, the maximum mycelium production was obtained. On the other hand, in the case of exopolysaccharide production, it was obtained at waste vegetable oil of 1.0g/L. Among various surfactants, Tween 80 was the best one for effect on the mycelium and exopolysaccharide production from submerged liquid fermentation of Pleurotus sajor-caju PSC 14. Fed batch fermentations were carried out in 20L airlift bioreactor containing 10L working volume. When Tween 80 was used, the maximum mycelium and exopolysaccharide concentration were respectively 5.62 g/L and 0.67g/L after 8 days of culture. The product yield from oil was 0.12g/g consumed carbon source. These results show that Tween 80 can be useful for the mycelium and exopolysaccharide production from submerged liquid fermentation of Pleurotus sajor-caju PSC 14 using waste vegetable oil.
Abstract
The object of this research is to study effect of the waste vegetable oil on mycelium and exopolysaccharide production from Pleurotus sajor-caju PSC 14. Among various culture temperatures, the mycelium and exopolysaccharide production were highest at 29°C. However, they decreased over and below 29°C. When waste vegetable oil 2.0g/L was used for 5 days, the maximum mycelium production was obtained. On the other hand, in the case of exopolysaccharide production, it was obtained at waste vegetable oil of 1.0g/L. Among various surfactants, Tween 80 was the best one for effect on the mycelium and exopolysaccharide production from submerged liquid fermentation of Pleurotus sajor-caju PSC 14. Fed batch fermentations were carried out in 20L airlift bioreactor containing 10L working volume. When Tween 80 was used, the maximum mycelium and exopolysaccharide concentration were respectively 5.62 g/L and 0.67g/L after 8 days of culture. The product yield from oil was 0.12g/g consumed carbon source. These results show that Tween 80 can be useful for the mycelium and exopolysaccharide production from submerged liquid fermentation of Pleurotus sajor-caju PSC 14 using waste vegetable oil.
참고문헌 (14)
- Dalonso, N., , Applied Biochemistry and Biotechnology, 160, pp.2265~2274 (2010)
- Dapeng, B., , Journal of Wood Science, 50, pp.162~168 (2004)
- Makropoulou, M., , Journal of Medicinal Food, 5, pp.216~221 (2012)
- Lavi, I., , Applied Microbiology and Biotechnology, 85, pp.1977~1990 (2009)
- Shashirekha, M., , Food Chemistry, 76, pp.27~31 (2002)
- Altieri, R., , International Biodeterioration &Biodegradation, 63, pp.993~997 (2009)
- Kim, J. M., , Journal of Industrial Microbiology and Biotechnology, 29, pp.292~295 (2002)
- Hyun Mi Kim, "Enhanced Production of Exopolysaccharides by Fed-batch Culture of Ganoderma resinaceum DG-6556", The Journal of Microbiology, 44(2), pp.233~242 (2006), 한국미생물학회
- Park, J. P., , Letters in Applied Microbiology, 33, pp.76~81 (2001)
- 이종석, "Effects of Ascorbic Acid and Uracil on Exo-polysaccharide Production with Hericium erinaceus in Liquid Culture", Biotechnology and Bioprocess Engineering, 15(3), pp.453~459 (2010), 한국생물공학회
- 나사룰라, "Effect of Reactor Surface on Production of Bacterial Cellulose and Water Soluble Oligosaccharides by Gluconacetobacter hansenii PJK", Biotechnology and Bioprocess Engineering, 15(1), pp.110~118 (2010), 한국생물공학회
- Chang, H.W., , Analytical Biochemistry, 104, pp.112~117 (1980)
- Kinoshita, K., , Nippon Nogeikagaku Kaishi, 43, pp.404~409 (1969)
- Li, Q., , Water Research, 33, pp.1090~1100 (1999)