Breadcrumb as a New Substrate for Trametes versicolor and Schizophyllum commune Submerged Cultivation
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A new substrate, breadcrumbs, was investigated for biomass accumulation, the pH of the cultural broth, the formation of primarymetabolites such as the proteins and endopolysaccharides of Schizophyllum commune 1768 and Trametes versicolor353, as well as its bioconversion efficiency. The results showed that S. commune gives more mycelial mass (23.96 ± 0.8 g/l)and in a shorter period (4 days) than T. versicolor (15.76 ± 0.5 g/l in 5 days). The pH values changed from the initial 6.1 to 3.6in S. commune cultural broth and to 4.4 in T. versicolor cultural broth. Maximal endopolysaccharide content in the mycelia of S. commune and T. versicolor were 7.13% and 6.42%, correspondingly. Crude protein content in S. commune mycelium was18.83 % on the 4th day of cultivation, and 20.03%, in the mycelium of T. versicolor, on the 6th day of cultivation. Kinetic parametersfor the quantitative estimation of cultivation efficiency were calculated for biomass, endopolysaccharide, and crude proteinconcentrations.
Abstract
A new substrate, breadcrumbs, was investigated for biomass accumulation, the pH of the cultural broth, the formation of primarymetabolites such as the proteins and endopolysaccharides of Schizophyllum commune 1768 and Trametes versicolor353, as well as its bioconversion efficiency. The results showed that S. commune gives more mycelial mass (23.96 ± 0.8 g/l)and in a shorter period (4 days) than T. versicolor (15.76 ± 0.5 g/l in 5 days). The pH values changed from the initial 6.1 to 3.6in S. commune cultural broth and to 4.4 in T. versicolor cultural broth. Maximal endopolysaccharide content in the mycelia of S. commune and T. versicolor were 7.13% and 6.42%, correspondingly. Crude protein content in S. commune mycelium was18.83 % on the 4th day of cultivation, and 20.03%, in the mycelium of T. versicolor, on the 6th day of cultivation. Kinetic parametersfor the quantitative estimation of cultivation efficiency were calculated for biomass, endopolysaccharide, and crude proteinconcentrations.
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