황 및 철산화균을 이용한 오염토양 중금속 용출 기술의 현장적용성을 위한 연구
Field Applicability Field Applicability of Heavy Metal Extraction Technique from Contaminated Soils using Sulfur or Iron Oxidizing Bacteria
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
황 및 철산화균을 이용하여 오염 토양으로부터 중금속을 용출한 그간의 연구는 대부분 실험실에서 미생물 활성에 유리한조건을 조성한 상태에서 수행되었다. 이 연구는 미생물학적 용출법을 현장 토양에 적용할 때 직면하는 기술적 문제들을 조사함으로써 현장적용성을 높이기 위한 목적으로 수행되었다. 반응 슬러리의 고액비를 1:3 정도로 대폭 증가시켜도 효과적인 중금속 용출이관찰되었다. 미생물학적 용출은 -200 mesh의 미립질 토양으로부터도 향상된 중금속 용출을 보였으며 특히 슬러리를 교반하였을 경우 한층 효과적이었다. 1,000 L에 이르는 대용량 배양조에서 미생물을 증균할 경우 순차적으로 용량을 증가시키며 배양하는 것이 효율적이며 최종 용량에서는 계대배양을 반복할수록 미생물의 활성도가 증가하였다. 철산화균의 에너지원으로 필요한 Fe2+투입량은표준 용량인 9 g/L보다 적어도 높은 효율을 보이므로 현장 적용 전 사전 연구를 통해 적정 투입량을 결정하는 것이 필요하다.
Abstract
Most studies on the bioleaching of heavy metals from contaminated soils using sulfur or iron-oxidizing bacteria have been conducted in laboratories under experimental conditions favorable for microbial activity. In this study, the applicability of bioleaching to field was investigated by examining various technical problems. Under the condition of a high solid-liquid ratio up to 1:3, the bioleaching showed a satisfactory efficiency. It resulted in high amounts of extracted heavy metals from fine soil particles under 200 mesh, and agitation of soil slurry led to an enhanced efficiency. For the preparation of a large volume of bacterial enrichment up to 1,000 L, a sequential increase of culture medium volume showed positive results, and subculture of bacteria in the final medium volume led to increase in bacterial activity. An input amount of Fe2+ less than 9 g/L also resulted in high bioleaching efficiency, thus a preliminary determination of appropriate Fe2+ amount may be required prior to field application.
참고문헌 (22)
- Danilo Borja, "Column Bioleaching of Arsenic from Mine Tailings Using a Mixed Acidophilic Culture: A Technical Feasibility Assessment", 자원리싸이클링, 24(6), pp.69~77 (2015), 한국자원리싸이클링학회[DOI]
- Couillard, D., "bacterial leaching of heavy metals from sewage sludge for agricultural application", Water Air Soil Pollut, 63(1-2), pp.67~80 (1991)
- Feng, G., "Metagenomic analysis of microbial community and function involved in cd-contaminated soil", BMC Microbiol, 18(1), pp.11~ (2018)
- Geng, H., "Leaching behavior of metals from iron tailings under varying pH and low molecularweight organic acids", J. Hazard. Mater, 383, pp.121136~ (2020)
- Gupta, A., "Metagenomic exploration of microbial community in mine tailings of Malanjkhand copper project, India", Genom. Data, 12, pp.11~13 (2017)
- 한협조, "Experimental study on bioleaching of paddy soil in the vicinity of refinery site contaminated with copper, lead, and arsenic using sulfur-oxidizing bacteria", Geosystem Engineering, 18(2), pp.79~84 (2015), 한국자원공학회
- 한협조, "제련소 주변 오염토양의 중금속 및 비소 용출을 위한 황산화균과 철산화균의 용출 특성 비교", 한국자원공학회지, 48(6), pp.713~722 (2011), 한국자원공학회
- 한협조, "황산화균 Acidithiobacillus thiooxidans를 이용한 사격장 토양 내 중금속 용출", 자원환경지질, 42(5), pp.457~469 (2009), 대한자원환경지질학회
- Kedziorek, M. A., "Leaching of Cd and Pb from a polluted soil during the percolation of EDTA : laboratory column experiments modeled with a non-equilibrium solubilization step", Environ. Sci. Technol, 32(11), pp.1609~1614 (1998)
- 김윤수, "미생물학적 황 산화에 의한 오염토양 내 중금속 및 비소의 용출", 한국자원공학회지, 48(3), pp.294~308 (2011), 한국자원공학회
- 고명수, "황산화균 Acidithiobacillus thiooxidans를 이용한 폐금은광산 광미에서의 중금속 용출", 한국자원공학회지, 46(2), pp.239~251 (2009), 한국자원공학회
- Ko, M. -S., "The role of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in arsenic bioleaching from soil", Environ. Geochem. Health, 35(6), pp.727~733 (2013)
- Lee, J. -U., "Microbial removal of uranium in uranium-bearing black shale", Chemosphere, 59(1), pp.147~154 (2005)
- Lee, K. Y., "Evaluation on the feasibility of microbially enhanced electrokinetic removal of multiple heavy metals from tailing soil", Sep. Sci. Technol, 44(10), pp.2322~2340 (2009)
- Mardanov, A. V., "Metagenome sequence of a microbial community from the gold mine tailings in the Kuzbass area, Russia", Genome Announc, 5(49), pp.e01355~17 (2017)
- Van Khanh Nguyen, "A Comparison of Microbial Leaching and Chemical Leaching of Arsenic and Heavy Metals from Mine Tailings", Biotechnology and Bioprocess Engineering, 20(1), pp.91~99 (2015), 한국생물공학회
- Park, H. -S., "The effects of Acidithiobacillus ferrooxidans on the leaching of cobalt and strontium adsorbed onto soil particles", Environ. Geochem. Health, 29(4), pp.303~312 (2007)
- Seh-Bardan, B. J., "Column bioleaching of arsenic and heavy metals from gold mine tailings by Aspergillus fumigatus", CLEAN Soil Air Water, 40(6), pp.607~614 (2012)
- Tunsu, C., "Recovery of critical materials from mine tailings : A comparative study of the solvent extraction of rare earths using acidic, solvating and mixed extractant systems", J. Clean. Prod, 218, pp.425~437 (2019)
- Tyagi, R. D., "Encyclopedia of Environmental Control Technology, Vol. 3", pp.557~591 (1989), Gulf Publishing Co
- Wasay, S. A., "Remediation of soils polluted by heavy metals using salts of organic acids and chelating agents", Environ. Technol, 19(4), pp.369~379 (1998)
- Zhang, X., "Metagenome-scale analysis yields insights into the structure and function of microbial communities in a copper bioleaching heap", BMC Genet, 17(1), pp.21~ (2016)