고추냉이 추출액의 항산화 효과
Anti-oxidant Effect of Wasabia Japonica Extracts
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
This study was carried out to investigate the anti-oxidant effect of Wasabia japonica(WJ). The anti-oxidant activities of various extracts from WJ were investigated. The water and ethanol extracts of WJ leaf were found to cause significant free radical scavenging effects on DPPH and nitric oxide(NO). Sprague-Dawley male rats were randomly assigned to one normal diet and three high cholesterol diet groups which contained 1%(w/w) cholesterol. After 4weeks cholesterol diet, the groups of high cholesterol diet were classified to control(normal diet only), WJR5(normal diet and 5% WJ root) and WJL5(normal diet and 5% WJ leaf). Liver xanthine oxidase(XO) activity in the rats of high cholesterol diet was decreased up to the levels of normal diet group according to WJ diet. The results indicated that WJ possesses antioxidant effects through free radical scavenging effects on DPPH, NO and the decreasing of XO activity. In view of these results, WJ is expected to be an effective material for the anti-oxidant.
Abstract
This study was carried out to investigate the anti-oxidant effect of Wasabia japonica(WJ). The anti-oxidant activities of various extracts from WJ were investigated. The water and ethanol extracts of WJ leaf were found to cause significant free radical scavenging effects on DPPH and nitric oxide(NO). Sprague-Dawley male rats were randomly assigned to one normal diet and three high cholesterol diet groups which contained 1%(w/w) cholesterol. After 4weeks cholesterol diet, the groups of high cholesterol diet were classified to control(normal diet only), WJR5(normal diet and 5% WJ root) and WJL5(normal diet and 5% WJ leaf). Liver xanthine oxidase(XO) activity in the rats of high cholesterol diet was decreased up to the levels of normal diet group according to WJ diet. The results indicated that WJ possesses antioxidant effects through free radical scavenging effects on DPPH, NO and the decreasing of XO activity. In view of these results, WJ is expected to be an effective material for the anti-oxidant.
참고문헌 (22)
- Yano T, "The effect of 6-methylthiohexyl isothiocyanate isolated from Wasabia japonica(wasabi) on 4- (methylnitrosamino)-1-(3-pyridyl)-1-buatnone-induced lung tumorigenesis in mice", Cancer Lett, 155, pp.115~120 (2000)
- Soledade M, "Phyoalexin accumulation and antifungal compounds from the Crucifer wasabi", Phytochemistry, 49, pp.1959~1965 (1998)
- Tajima H, "Effects of synthetic hydroxy isothiocyanates on microbial systems", Biosci Biotechnol Biochem, 62, pp.491~495 (1998)
- 황광진, "산화질소(Nitric Oxide) 이로운가? 해로운가?:산화질소의 화학과 응용", 대한화학회지, 39, pp.52~63 (1999)
- 오명숙, "DPPH 방법을 통한 토사자, 보골지(補骨脂), 사상자(蛇床子), 음양곽(淫羊藿)의 항산화 활성에 대한 연구", 대한한의학방제학회지, 13(2), pp.101~110 (2005)
- 김성자, "메밀 지상부의 DPPH 라디칼 소거작용과 활성 플라보노이드의 분리", 대한한의학방제학회지, 12, pp.255~262 (2004)
- Seo WG, "Inhibitory effect of ethyl acetate fraction from Cudrania tricuspidata on the expression of nitric oxide synthase gene in RAW 264.7 macrophages stimulated with interferon- and lipopolysaccharide", Gen Pharmacol, 35, pp.21~28 (2000)
- Chiou WF, "Camp- tothecin suppresses nitric oxide biosyn- thesis in RAW 264.7 macrophages", Life Sci, 69, pp.625~635 (2001)
- Lee BG, "Suppression of inducible nitric oxide synthase expression in RAW 264.7 macrophages by two- carboline alkaloids extracted from Melia azedarach", Eur J Pharmacol, 406, pp.301~309 (2000)
- Seo WG, "The aqueous extract of Rhodiola sachalinensis root enhances the expre- ssion of inducible nitric oxide synthase gene in RAW 264.7 macrophages", J Ethno- pharmacol, 76, pp.119~123 (2001)
- Kawamata H, "Enhanced expression of inducible nitric oxide synthase by Juzen- taiho-to in LPS-activated RAW 264.7 cells, a murine macrophage cell line", Am J Chin Med, 28, pp.217~226 (2000)
- Ishihara T, "Poly- gonum tinctorium extract suppresses nitric oxide production by activated macro- phages through inhibiting inducible nitric oxide synthase expression", J Ethnophar- macol, 72, pp.141~150 (2000)
- Kim NY, "Inhibitory effects of butanol fraction of the aqueous extract of Forsythia koreana on the nitric oxide production by murine macrophage-like RAW 264.7 cells", J Ethnopharmacol, 73, pp.323~327 (2000)
- Seo WG, "Inhibitory effects of methanol extract of Cyperus rotundus rhizomes on nitric oxide and superoxide production by murine macrophage cell line, RAW 264.7 cells", J Ethnopharmacol, 76, pp.59~64 (2001)
- Blois MS, "Antioxidant determinations by the use of stable free radical", Nature, 26, pp.1199~1200 (1958)
- Marcocci PL, "antioxidant action of Ginkgo biloba extract EGP761", Methods in Enzymology, 234, pp.462~475 (1994)
- Della Corte E, "The regul- ation of rat-liver xanthine oxidase:Acti- vation by proteolytic enzymes", FEBS Lett, 2, pp.83~84 (1968)
- Watanabe M, "Identification of 6-methylsulfinylhexyl isothiocyanate as an apoptosis-inducing component in wasabi", Phytochemistry, 62, pp.733~739 (2003)
- Morimitsu Y, "Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish Wasabi", Mech Ageing Dev, 116, pp.125~134 (2000)
- Chiang HC, "Xanthine oxidase inhibitors from the leaves of Alsophila spinulosa(Hook) Tryon", J Enzyme Inhib, 8, pp.61~71 (1994)
- Cos P, "Structure- activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers", J Nat Prod, 61, pp.71~76 (1998)
- Thomson MJ, "Atherosclerosis and oxidant stress:the end of the road for antioxidant vitamin treatment?", Cardiovasc Drugs Ther, 21, pp.195~210 (2007)