아토피 피부염 동물모델에서 피부에 도포한 한약 복합추출물의 염증 억제 효과
Topical Application of Herbal-mixed Extract on back skin suppresses inflammation in Ovalbumin-induced Atopic Dermatitis
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초록
Objective : This study is conducted to evaluate the efficiency of ethanol extracts of 4 mixed herbs(CP001or CP002) on mouse model of atopic dermatitis induced by ovalbumin. Methods : Female BALB/c mice were internally sensitized with ovalbumin(20㎍) plus aluminum hydroxide hydrate(4㎎) once per week. After 3 weeks, they were dermally challenged with patches containing ovalbumin (100㎍) plus aluminum hydroxide hydrate(20㎎) every other day for 3 weeks. After induction of atopic dermatitis, mice back skin were gently rubbed with CP001 or CP002(200㎎/㎖, 100㎕) for two weeks(every 2days). Results : In CP001 or CP002 treated group, there was a remarkable reduction in infiltration of eosinophils on the skin areas and diminution of mast cells and total T cells in blood samples as compared with control group. Cutaneous expressions of interleukin-13, 17 were also decreased by CP001 or CP002. Moreover,blood immunoglobulin E level was decreased by drug administration while there was no decrease in OVA sensitization group. Conclusion : In summary, our result shows that herbal extracts(CP001 and CP002) could be potential candidates for the treatment of chronic atopic dermatitis.
Abstract
Objective : This study is conducted to evaluate the efficiency of ethanol extracts of 4 mixed herbs(CP001or CP002) on mouse model of atopic dermatitis induced by ovalbumin. Methods : Female BALB/c mice were internally sensitized with ovalbumin(20㎍) plus aluminum hydroxide hydrate(4㎎) once per week. After 3 weeks, they were dermally challenged with patches containing ovalbumin (100㎍) plus aluminum hydroxide hydrate(20㎎) every other day for 3 weeks. After induction of atopic dermatitis, mice back skin were gently rubbed with CP001 or CP002(200㎎/㎖, 100㎕) for two weeks(every 2days). Results : In CP001 or CP002 treated group, there was a remarkable reduction in infiltration of eosinophils on the skin areas and diminution of mast cells and total T cells in blood samples as compared with control group. Cutaneous expressions of interleukin-13, 17 were also decreased by CP001 or CP002. Moreover,blood immunoglobulin E level was decreased by drug administration while there was no decrease in OVA sensitization group. Conclusion : In summary, our result shows that herbal extracts(CP001 and CP002) could be potential candidates for the treatment of chronic atopic dermatitis.
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