Determination of Genetic Diversity among Korean Hanwoo Cattle Based on Physical Characteristics
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
This study was conducted to establish genetic criteria for phenotypic characteristics of Hanwoo cattle based on allele frequencies and genetic variance analysis using microsatellite markers. Analysis of the genetic diversity among 399 Hanwoo cattle classified according to nose pigmentation and coat color was carried out using 22 microsatellite markers. The results revealed that the INRA035 locus was associated with the highest F_is (0.536). Given that the F_is value for the Hanwoo INRA035 population ranged from 0.533 (white) to 1.000 (white spotted), this finding was consistent with the loci being fixed in Hanwoo cattle. Expected heterozygosities of the Hanwoo groups classified by coat colors and degree of nose pigmentation ranged from 0.689±0.023 (Holstein) to 0.743±.021 (nose pigmentation level of d). Normal Hanwoo and animals with a mixed white coat showed the closest relationship because the lowest D_A value was observed between these groups. However, a pair-wise differentiation test of F_st showed no significant difference among the Hanwoo groups classified by coat color and degree of nose pigmentation (p<0.01). Moreover, results of the neighbor-joining tree based on a DA genetic distance matrix within 399 Hanwoo individuals and principal component analyses confirmed that different groups of cattle with mixed coat color and nose pigmentation formed other specific groups representing Hanwoo genetic and phenotypic characteristics. The results of this study support a relaxation of policies regulating bull selection or animal registration in an effort to minimize financial loss, and could provide basic information that can be used for establishing criteria to classify Hanwoo phenotypes.
Abstract
This study was conducted to establish genetic criteria for phenotypic characteristics of Hanwoo cattle based on allele frequencies and genetic variance analysis using microsatellite markers. Analysis of the genetic diversity among 399 Hanwoo cattle classified according to nose pigmentation and coat color was carried out using 22 microsatellite markers. The results revealed that the INRA035 locus was associated with the highest F_is (0.536). Given that the F_is value for the Hanwoo INRA035 population ranged from 0.533 (white) to 1.000 (white spotted), this finding was consistent with the loci being fixed in Hanwoo cattle. Expected heterozygosities of the Hanwoo groups classified by coat colors and degree of nose pigmentation ranged from 0.689±0.023 (Holstein) to 0.743±.021 (nose pigmentation level of d). Normal Hanwoo and animals with a mixed white coat showed the closest relationship because the lowest D_A value was observed between these groups. However, a pair-wise differentiation test of F_st showed no significant difference among the Hanwoo groups classified by coat color and degree of nose pigmentation (p<0.01). Moreover, results of the neighbor-joining tree based on a DA genetic distance matrix within 399 Hanwoo individuals and principal component analyses confirmed that different groups of cattle with mixed coat color and nose pigmentation formed other specific groups representing Hanwoo genetic and phenotypic characteristics. The results of this study support a relaxation of policies regulating bull selection or animal registration in an effort to minimize financial loss, and could provide basic information that can be used for establishing criteria to classify Hanwoo phenotypes.
참고문헌 (52)
- Aasland, M., "Two polymorphisms in the bovine mast cell growth factor (MGF)", Anim. Genet, 31, pp.346~ (2000)
- Boom, R., "Rapid and simple method for purification of nucleic acids", J. Clin. Microbiol, 28, pp.495~503 (1990)
- Cañón, J., "Genetic diversity measures of local European beef cattle breeds for conservation purposes", Genet. Sel. Evol, 33, pp.311~332 (2001)
- Cítek, J., "Study of genetic distances between cattle breeds of Central Europe", Czech J. Anim. Sci, 51, pp.429~436 (2006)
- Cockerham, C. C., "Estimation of gen flow from F-statistics", Evolution, 47, pp.855~863 (1993)
- El Mousadik, A., "High level of genetic differentiation for allelic richness among population of the tree (Argania spinosa(L.) Skeels) endemic to Morocco", Theor. Appl. Genet, 92, pp.832~839 (1996)
- Felsenstein, J., "How can we infer geography and history from gene frequencies?", J. Theor. Biol, 96, pp.9~20 (1982)
- Felsenstein, J., "PHYLIP. Version. 3.67", Department of Genetics, pp.2007
- "FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3)" (2001)[URL]
- Ibeagha-Awemu, E. M., "Genetic diversity, introgression and relationships among West", Central Africa cattle breed, 2004, pp.36, 673~690
- 장요순, "소 c-KIT Receptor 유전자의 다형성에 관한 연구", 한국동물자원과학회지, 44(6), pp.653~660 (2002), 한국동물자원과학회
- Jordana, J., "Genetic structure of eighteen local south european beef cattle breeds on microsatellite data", Anim. Genet, 33, pp.201~204 (2003)
- Kim, K. S., "Genetic diversity of Korean cattle using microsatellite analysis", J. Anim. Sci. Technol. (Kor.), 43, pp.599~608 (2001)
- Korea Animal Improvement Association, "Standard of judging Hanwoo (public notice 97-7)", ~ (1997)
- Laval, G., "Measuring genetic distances between breeds: use of some distances in various shortterm evolution models", Genet. Sel. Evol, 34, pp.481~507 (2002)
- 이성수, "칡소와 비경흑색 한우의 Melanocortin Receptor 1 (MC1R)유전자형 분석", 한국동물자원과학회지, 44(1), pp.23~30 (2002), 한국동물자원과학회
- Leipold, H. W., "Complete albinism in a Guernsey calf", J. Hered, 59, pp.218~220
- Lirón, J. P., "Genetic characterization of Argentine and Bolivian Creole cattle breeds assessed through microsatellites", J. Hered, 97, pp.331~339 (2006)
- Loftus, R. T., "A microsatellite survey of cattle from a centre of origin: the Near East", Mol. Ecol, 8, pp.2015~2022 (1999)
- MacHugh, D. E., "Genetic structure of seven European cattle breeds assessed using 20 microsatellite markers", Anim. Genet, 29, pp.333~340 (1998)
- Martín-Burriel, I., "Genetic diversity analysis of six Spanish native cattle breeds using microsatellites", Anim. Genet, 3, pp.177~182 (1999)
- Martín-Burriel, I., "Genetic diversity and relationships of endangered Spanish cattle breeds", J. Hered, 98, pp.697~700 (2007)
- Mateus, J. C., "Genetic diversity and differentiation in Portuguese cattle breeds using microsatellites", Anim. Genet, 35, pp.106~113 (2004)
- Minch, E., "MICROSAT. Version 1.5b" (1998), University of Stanford
- Moazami-Goudarzi, K., "Analysis of genetic relationships between 10 cattle breeds with 17 microsatellites", Anim. Genet, 28, pp.338~345 (1997)
- Mukesh, M., "Genetic diversity of Indian native cattle breeds as analysed with 20 microsatellite loci", J. Anim. Breed. Genet, 121, pp.416~424 (2004)
- Nei, M., "Accuracy of estimated phylogenetics trees from molecular data", J. Mol. Evol, 19, pp.153~170 (1983)
- Nei, M., "Molecular evolutionary genetics" (1987), Columbia Univ
- Olsen, T., "The genetic basis for piebald patterns in cattle", J. Hered, 72, pp.113~116 (1981)
- Olson, T. A., "Genetics of color variation. In: The Genetics of Cattle", pp.33~ (1999), CABI
- Ota, T., "DISPAN" (1993), Pennsylvania State University
- Pandey, A. K., "Genetic diversity studies of Kherigarh cattle based on microsatellite markers", J. Genet, 85, pp.117~122 (2006)
- Park, S. D. E., "Trypanotolerane in west African cattle and the population genetic effects of selection", 박사, pp.2001
- Pawson, T., "Receptor tyrosine kinase: genetic evidence for their role in Drosphila and mouse development", Trends Genet, 6, pp.350~356 (1990)
- Philippe, H., "Overview of the first phylogenomics conference", BMC Evol Biol, 7(supple 1), pp.S1~ (2007)
- Rao, C., "A defect in a novel ADAMTS family member is the cause of the belted white-spotting mutation", Development, 130, pp.4665~ (2003)
- Reinsch, N., "A QTL for the degree of spotting in cattle shows synteny with the KIT locus on chromosome 6", J. Hered, 90, pp.629~634 (1999)
- Roh, S. H., "Studies on selection efficiency using ultrasound measurement trait in Hanwoo (Korean native cattle)", 박사, Division of Animal Sciences, pp.2008
- Rosenberg, N. A., "Empirical evaluation of genetic clustering methods using multilocus genotypes from 20 chicken breeds", Genetics, 159, pp.699~713 (2001)
- Saitou, N., "The neighbor-joining method: a new method for reconstrucing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
- Schmid, M., "Genetic diversity in Swiss cattle breeds", J. Anim. Breed. Genet, 116, pp.1~8 (1999)
- Schmutz, S. M., "A form of albinism in cattle is caused by a tyrosinase frame shift mutation", Mamm. Genome, 15, pp.62~67 (2004)
- Seitz, J. J., "A missense mutation in the bovine MGF gene is associated with the roan phenotype in Belgian Blue and Shorthorn cattle", Mamm. Genome, 10, pp.710~712 (1999)
- Seo, K., "Biology of epidermal and hair pigmentation in cattle: a mini-review", Veterinary Dermatol, 18, pp.392~400 (2007)
- Sodhi, M., "Microsatellite DNA typing for assessment of genetic variability in Tharparkar breed of Indian Zebu (Bos indicus) cattle, a major breed of Rajasthan", J. Genet, 85, pp.165~170 (2006)
- Takezaki, N., "Genetic distances and reconstruction of phylogenetic trees from microsatellite DNA", Genetics, 144, pp.389~399 (1996)
- Vijh, R. K., "Genetic relationship and diversity analysis of Indian water buffalo (Bubalus bubalis)", J. Anim. Sci, 86, pp.1495~1502 (2008)
- Weir, B. S., "Genetic data analysis" (1990), Sunderland
- Weir, B. S., "Genetic data analysis II: methods for discrete population genetic data" (1996), Sunderland
- Yoon, D. H., "Molecular genetic diversity and development of genetic markers in association with meat quality for Hanwoo (Korean cattle)", 박사, Dept. of Animal Sciences, pp.2002
- 윤두학, "Microsatellite loci 분석에 의한 한우와 타 품종간의 유전적 유연관계", 한국동물자원과학회지, 47(3), pp.341~354 (2005), 한국동물자원과학회
- Zhang, G. X., "Genetic diversity and population structure of indigenous yellow cattle breeds of China using 30 microsatellite markers", Anim. Genet, 38, pp.550~559 (2007)