Microsatellite loci 분석을 통한 한우 지역 브랜드간 유전적 다양성의 비교
Comparison for Genetic Diversity between Regional Hanwoo (Korean Cattle) Brand Group Using Microsatellite Loci
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초록
Genotype data of eleven microsatellites typed in 713 Hanwoo breed(Korean cattle) animals were used to estimate the genetic diversities and relationship of nine brand populations distributed in six regional areas. Size of microsatellite markers decided using GeneMapper Software(v.4.0) after analyze in kinds of ABI machine of name of 3130 using ABI Stock MarkersTM kit. Frequencies of microsatellites markers were used to estimate heterozygosities, gene diversities and genetic distances. Microsatellite markers showed high polymorphism and 163 alleles were detected from the genotyping of eleven microsatellite markers. Allele frequency results were resemblant to each other in case of most markers and this between each brand where several alleles shared the majority mean. Genetic distances between populations were obtained using Nei’s DA distance method. Expected heterozygosity between each brand population was estimated very analogously. Average expected heterozygosity(0.765) of whole Hanwoo populations had higher diversities than other species of Europe, North American continent and spanish breeds. Genetic distances(0.0413) between ChoneBuk(JS) and ChoneNam(SJ) was nearest than distances between other populations. Genetic distances between KyongGi(AS, YP) and KyongNam(NH) showed far distance than other populations by 0.0493 and 0.0489 each respectively. ChoneNam(SJ) showed near genetic distance relatively with other areas' populations. In the UPGMA tree that is made based on DA distance matrix, some brand populations ramified to area. Because this became evenly genetic because gene flow of Hanwoo was frequent comparatively between each area by stock bull selection of country unit and their sperms(KPN) supply system that is done till today. It can be considered that this effect is by transfer of calf between near geographical areas. Each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all Hanwoo of each brand.
Abstract
Genotype data of eleven microsatellites typed in 713 Hanwoo breed(Korean cattle) animals were used to estimate the genetic diversities and relationship of nine brand populations distributed in six regional areas. Size of microsatellite markers decided using GeneMapper Software(v.4.0) after analyze in kinds of ABI machine of name of 3130 using ABI Stock MarkersTM kit. Frequencies of microsatellites markers were used to estimate heterozygosities, gene diversities and genetic distances. Microsatellite markers showed high polymorphism and 163 alleles were detected from the genotyping of eleven microsatellite markers. Allele frequency results were resemblant to each other in case of most markers and this between each brand where several alleles shared the majority mean. Genetic distances between populations were obtained using Nei’s DA distance method. Expected heterozygosity between each brand population was estimated very analogously. Average expected heterozygosity(0.765) of whole Hanwoo populations had higher diversities than other species of Europe, North American continent and spanish breeds. Genetic distances(0.0413) between ChoneBuk(JS) and ChoneNam(SJ) was nearest than distances between other populations. Genetic distances between KyongGi(AS, YP) and KyongNam(NH) showed far distance than other populations by 0.0493 and 0.0489 each respectively. ChoneNam(SJ) showed near genetic distance relatively with other areas' populations. In the UPGMA tree that is made based on DA distance matrix, some brand populations ramified to area. Because this became evenly genetic because gene flow of Hanwoo was frequent comparatively between each area by stock bull selection of country unit and their sperms(KPN) supply system that is done till today. It can be considered that this effect is by transfer of calf between near geographical areas. Each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all Hanwoo of each brand.
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