Different Phosphate Transport in the Duodenum and Jejunum of Chicken Response to Dietary Phosphate Adaptation
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Intestinal phosphate (Pi) absorption across the apical membrane of small intestinal epithelial cells is mainly mediated by the type IIb Na-coupled phosphate co-transporter (NaPi-IIb), but its expression and regulation in the chicken remain unclear. In the present study, we investigated the mRNA and protein levels of NaPi-IIb in three regions of chicken small intestine, and related their expression levels to the rate of net phosphate absorption. Our results showed that maximal phosphate absorption occurs in the jejunum, however the highest expression levels of NaPi-IIb mRNA and protein occurs in the duodenum. In response to a low-Pi diet (TP 0.2%), there is an adaptive response restricted to the duodenum, with increased brush border membrane (BBM) Na-Pi transport activity and NaPi-IIb protein and mRNA abundance. However, when switched from a low- (TP 0.2%) to a normal diet (TP 0.6%) for 4 h, there is an increase in BBM NaPi-IIb protein abundance in the jejunum, but no changes in BBM NaPi-IIb mRNA. Therefore, our study indicates that Na-Pi transport activity and NaPi-IIb protein expression are differentially regulated in the duodenum vs the jejunum in the chicken.
Abstract
Intestinal phosphate (Pi) absorption across the apical membrane of small intestinal epithelial cells is mainly mediated by the type IIb Na-coupled phosphate co-transporter (NaPi-IIb), but its expression and regulation in the chicken remain unclear. In the present study, we investigated the mRNA and protein levels of NaPi-IIb in three regions of chicken small intestine, and related their expression levels to the rate of net phosphate absorption. Our results showed that maximal phosphate absorption occurs in the jejunum, however the highest expression levels of NaPi-IIb mRNA and protein occurs in the duodenum. In response to a low-Pi diet (TP 0.2%), there is an adaptive response restricted to the duodenum, with increased brush border membrane (BBM) Na-Pi transport activity and NaPi-IIb protein and mRNA abundance. However, when switched from a low- (TP 0.2%) to a normal diet (TP 0.6%) for 4 h, there is an increase in BBM NaPi-IIb protein abundance in the jejunum, but no changes in BBM NaPi-IIb mRNA. Therefore, our study indicates that Na-Pi transport activity and NaPi-IIb protein expression are differentially regulated in the duodenum vs the jejunum in the chicken.
참고문헌 (30)
- Arima, K., "Glucocorticoid regulation and glycosylation of mouse intestinal type IIb Na-Pi cotransporter during ontogeny", Am. J. Physiol. Gastrointest. Liver Physiol, 283, pp.G426~G434 (2002)
- Bai, L., "Cloning and characterization of a type III Na-dependent phosphate cotransporter from mouse intestine", Am. J. Physiol. Cell Physiol, 279, pp.C1135~C1143 (2000)
- Blahos, J., "The jejunumis the site of maximal rate of intestinal absorption of phosphate in chicks", Physiol. Bohemoslov, 30, pp.157~159 (1981)
- Borowitz, S. M., "Phosphate transport in human jejunal bnrsh border membrane vesicles", Gastroenterology, 96, pp.4~10 (1989)
- Borowitz, S. M., "Ontogency of intestinal phosphate absorption in rabbits", Am. J. Physiol, 262, pp.G847~G853 (1992)
- Collins, J. F., "Molecular cloning, functional expression, tissue distribution, and in situ hybridization of the renal sodium phosphate (Na+", Pi) transporter in the control and hypophosphatemic mouse, 1994, pp.8, 862~868
- Cross, H. S., "Mechanism and regulation of intestinal phosphate absorption", Miner. Electrolyte Metab, 16, pp.115~124 (1990)
- Ghishan, F. K., "Intestinal phosphate transport in spontaneously hypertensive rats and genetically matched controls", Gastroenterology, 99, pp.106~112 (1990)
- Hashimoto, M., "Isolation and localization of type IIb Na", Pi cotransporter in the developing rat lung, 2000, pp.157, 21~27
- Hattenhaur, O., "Regulation of small intestinal Na-Pi type IIb cotransporter by dietary phosphate intake", Am. J. Physiol. Gasreoinrest. Liver Physiol, 277, pp.G756~G762 (1999)
- Heaney, R.P, "Calcium effects nn phnsphoius absorption:implications for the prevention and co-therapy of osteoporosis", J. Am. Coll. Nutr, 21, pp.239~204 (2002)
- Hector Giral, "Regulation of rat intestinal Na-dependent phosphate transporters by dietary phosphate", Am. J. Physiol. Renal Physiol, 297, pp.1466~1475 (2009)
- Hilfiker, H., "Characterization of a murine type II sodiumphosphate cotransporter expressed in mammalian intestine", Proc. Natl. Acad. Sci. USA, 95, pp.14564~14569 (1998)
- Huber, K., "Phosphate transport in the duodenum and jejunum of goats and its adaptation by dietary phosphate and calcium", Am. J. Physiol. Regul. Integr. Comp. Physiol, 283, pp.R296~R302 (2002)
- Hurwitz, S., "The sites of calcium and phosphate absorption in the chick", Poult. Sci, 49, pp.324~325 (1970)
- Kiyamova, R., "Development of monoclonal antibodis specific for the human sodium-dependent phosphate cotransporter NaPi2b", Hybridoma, 27, pp.277~284 (2008)
- Magagnin, S., "Expression cloning of human and rat renal cortex Na", Pi cotransport, 1993, pp.90, 5979~5983
- McHaffie, G. S., "The role of an intracellular cysteine stretch in the sorting of the type II Na", phosphate cotransporter, 2007, pp.1768, 2099~2106
- Miyamoto, K., "Cloning and functional expression of a Na-dependent phosphate cotransporter from human kidney: cDNA cloning and functional expression", Biochem. J, 301, pp.81~85 (1995)
- Murer, H., "The sodium phosphate cotransporter family SLC34", Pflugers. Arch, 447, pp.763~767 (2004)
- Nakagawa, N., "Low phosphate diet upregulates the renal and intestinal sodium-dependent phosphate transporter in vitamin D-resistant hypophosphatemic mice", Proc. Soc. Exp. Biol. Med, 205, pp.162~167 (1994)
- Palmada, M., "Regulation of intestinal phosphate cotransporter NaPi IIb by ubiquitin ligase Nedd4-2 and by serum- and glucocorticoid-dependent kinase 1", Am. J. Physiol. Gastrointest. Liver Physiol, 287, pp.G143~G150 (2004)
- Quamme, G. A., "Phosphate transport in intestinal brush-border membrane vesicles: effect of pH and dietary phosphate", Am. J. physiol, 249, pp.G168~G176 (1985)
- Radanovic, T., "Regulation of intestinal phosphate transport. I. Segmental expression and adaptation to low-P (i) diet of the type IIb Na+-Pi cotransporter in mouse small intestine", Am. J. Physiol, 288, pp.G496~G500 (2005)
- Saddoris, K. L., "Sodium-dependent phosphate uptake in the jejunum is post-transcriptionally regulated in pigs fed a low-phosphorus diet and is independent of dietary calcium concentration", J. Nutr, 4, pp.731~736 (2010)
- Segawa, H., "Intestinal Na-Pi cotranspotter adaptation to dietuy Pi content in vitamin D receptor null mice", Am. J. Physiol. Renal Physiol, 287, pp.F39~F47 (2004)
- Sugiura, S. H., "Molecular and conventional responses of large rainbow trout to dietary phosphorus restriction", J. Comp. Physiol. B, 177, pp.461~472 (2007)
- Villa-Bellosta, R., "Compensatory regulation of the sodium", phosphate cotransporters NaPi-IIc (SCL34A3) and Pit-2 (SLC20A2) during Pi deprivation and acidosis, 2010, pp.459, 499~508
- Xu, H., "Age-dependent regulation of rat intestinal type IIb sodium-phosphate cotransporter by 1, 25-(OH) 2 vitamin D3", Am. J. Physiol. Cell Physiol, 282, pp.C487~C493 (2002)
- Yan, F., "Characterization of the chicken small intestine type IIb sodium phosphate cotransporter", Poult. Sci, 86, pp.67~76 (2007)