Available phosphorus levels modulate gene expression related to intestinal calcium and phosphorus absorption and bone parameters differently in gilts and barrows
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Objective: Dietary phytase increases bioavailability of phytate-bound phosphorus (P) in pig nutrition affecting dietary calcium (Ca) to P ratio, intestinal uptake, and systemic utilization of both minerals, which may contribute to improper bone mineralization. We used phytase to assess long-term effects of two dietary available P (aP) levels using a one-phase feeding system on gene expression related to Ca and P homeostasis along the intestinal tract and in the kidney, short-chain fatty acids in stomach, cecum, and colon, serum, and bone parameters in growing gilts and barrows. Methods: Growing pigs (37.9±6.2 kg) had either free access to a diet without (Con; 75 gilts and 69 barrows) or with phytase (650 phytase units; n = 72/diet) for 56 days. Samples of blood, duodenal, jejunal, ileal, cecal, and colonic mucosa and digesta, kidney, and metacarpal bones were collected from 24 pigs (6 gilts and 6 barrows per diet). Results: Phytase decreased daily feed intake and average daily gain, whereas aP intake increased with phytase versus Con diet (p<0.05). Gilts had higher colonic expression of TRPV5, CDH1, CLDN4, ZO1, and OCLN and renal expression of TRPV5 and SLC34A3 compared to barrows (p<0.05). Phytase increased duodenal expression of TRPV5, TRPV6, CALB1, PMCA1b, CDH1, CLDN4, ZO1, and OCLN compared to Con diet (p<0.05). Furthermore, phytase increased expression of SCL34A2 in cecum and of FGF23 and CLDN4 in colon compared to Con diet (p<0.05). Alongside, phytase decreased gastric propionate, cecal valerate, and colonic caproate versus Con diet (p<0.05). Phytase reduced cortical wall thickness and index of metacarpal bones (p<0.05). Conclusion: Gene expression results suggested an intestinal adaptation to increased dietary aP amount by increasing duodenal trans- and paracellular Ca absorption to balance the systemically available Ca and P levels, whereas no adaption of relevant gene expression in kidney occurred. Greater average daily gain in barrows related to higher feed intake.
Abstract
Objective: Dietary phytase increases bioavailability of phytate-bound phosphorus (P) in pig nutrition affecting dietary calcium (Ca) to P ratio, intestinal uptake, and systemic utilization of both minerals, which may contribute to improper bone mineralization. We used phytase to assess long-term effects of two dietary available P (aP) levels using a one-phase feeding system on gene expression related to Ca and P homeostasis along the intestinal tract and in the kidney, short-chain fatty acids in stomach, cecum, and colon, serum, and bone parameters in growing gilts and barrows. Methods: Growing pigs (37.9±6.2 kg) had either free access to a diet without (Con; 75 gilts and 69 barrows) or with phytase (650 phytase units; n = 72/diet) for 56 days. Samples of blood, duodenal, jejunal, ileal, cecal, and colonic mucosa and digesta, kidney, and metacarpal bones were collected from 24 pigs (6 gilts and 6 barrows per diet). Results: Phytase decreased daily feed intake and average daily gain, whereas aP intake increased with phytase versus Con diet (p<0.05). Gilts had higher colonic expression of TRPV5, CDH1, CLDN4, ZO1, and OCLN and renal expression of TRPV5 and SLC34A3 compared to barrows (p<0.05). Phytase increased duodenal expression of TRPV5, TRPV6, CALB1, PMCA1b, CDH1, CLDN4, ZO1, and OCLN compared to Con diet (p<0.05). Furthermore, phytase increased expression of SCL34A2 in cecum and of FGF23 and CLDN4 in colon compared to Con diet (p<0.05). Alongside, phytase decreased gastric propionate, cecal valerate, and colonic caproate versus Con diet (p<0.05). Phytase reduced cortical wall thickness and index of metacarpal bones (p<0.05). Conclusion: Gene expression results suggested an intestinal adaptation to increased dietary aP amount by increasing duodenal trans- and paracellular Ca absorption to balance the systemically available Ca and P levels, whereas no adaption of relevant gene expression in kidney occurred. Greater average daily gain in barrows related to higher feed intake.
참고문헌 (28)
- Skiba G, "Femur morphometry, densitometry, geometry and mechanical properties in young pigs fed a diet free of inorganic phosphorus and supplemented with phytase", Arch Anim Nutr, 71, pp.81~92 (2017)[DOI]
- Dersjant-Li Y, "Phytase in non-ruminant animal nutrition : a critical review on phytase activities in the gastrointestinal tract and influencing factors", J Sci Food Agric, 95, pp.878~896 (2015)[DOI]
- Miller HM, "High dietary inclusion levels of phytase in grower-finisher pigs", J Anim Sci, 94, pp.121~124 (2016)[DOI]
- J.C. Gonzalez-Vega, "Calcium Digestibility and Metabolism in Pigs", Animal Bioscience, 27(1), pp.1~9 (2014), 아세아·태평양축산학회[DOI]
- Julia C Vötterl, "Evaluation of serum parameters to predict the dietary intake of calcium and available phosphorus in growing pigs", Translational Animal Science, 5(2) (2021), Oxford University Press (OUP)[DOI]
- Gerlinger C, "Physiological and transcriptional responses in weaned piglets fed diets with varying phosphorus and calcium levels", Nutrients, 11, pp.436~ (2019)[DOI]
- Breves G, "Transport of nutrients and electrolytes across the intestinal wall in pigs", Livest Sci, 109, pp.4~13 (2007)[DOI]
- Kiela PR, "Physiology of intestinal absorption and secretion", Best Pract Res Clin Gastroenterol, 30, pp.145~159 (2016)[DOI]
- Taylor JG, "Calcium and phosphorus homeostasis", Blood Purif, 27, pp.387~394 (2009)[DOI]
- Klinsoda J, "Alterations of the viable ileal microbiota of the gut mucosa-lymph node axis in pigs fed phytase and lactic acid-treated cereals", Appl Environ Microbiol, 86, pp.e02128~ (2020)[DOI]
- Metzler-Zebeli BU, "Maturational changes alter effects of dietary phytase supplementation on the fecal microbiome in fattening pigs", Microorganisms, 8, pp.1073~ (2020)[DOI]
- Schneider S, "Feed calculation for pigs" (2019), Bayerische Landesanstalt für Landwirtschaft
- Committee on Nutrient Requirements of Swine, "Nutrient requirements of swine" (2012), National Academy Press
- Vötterl JC, "Dietary phytase and lactic acid-treated cereal grains differently affected calcium and phosphorus homeostasis from intestinal uptake to systemic metabolism in a pig model", Nutrients, 12, pp.1542~ (2020)[DOI]
- Hegde S, "Microbiota dysbiosis and its pathophysiological significance in bowel obstruction", Sci Rep, 8, pp.13044~ (2018)[DOI]
- Olsen KM, "Evaluating phosphorus release by phytase in diets fed to growing pigs that are not deficient in phosphorus", J Anim Sci, 97, pp.327~337 (2019)[DOI]
- Sulabo RC, "Factors affecting storage stability of various commercial phytase sources", J Anim Sci, 89, pp.4262~4271 (2011)[DOI]
- Carbonare LD, "Bone microarchitecture as an important determinant of bone strength", J Endocrinol Invest, 27, pp.99~105 (2004)[DOI]
- Rupp T, "High FGF23 levels are associated with impaired trabecular bone microarchitecture in patients with osteoporosis", Osteoporos Int, 30, pp.1655~1662 (2019)[DOI]
- Metzler-Zebeli BU, "Explorative study of metabolic adaptations to various dietary calcium intakes and cereal sources on serum metabolome and hepatic gene expression in juvenile pigs", Metabolomics, 11, pp.545~558 (2015)[DOI]
- Zemel MB, "Role of calcium and dairy products in energy partitioning and weight management", Am J Clin Nutr, 79, pp.907~ (2004)[DOI]
- Malagutti L, "Effects of phytase on growth and slaughter performance, digestibility and nitrogen and mineral balance in heavy pigs", Ital J Anim Sci, 11, pp.e70~ (2012)[DOI]
- Van den Broeke A, "Effect of dietary energy level in finishing phase on performance, carcass and meat quality in immunocastrates and barrows in comparison with gilts and entire male pigs", Animal, 16, pp.100437~ (2022)[DOI]
- Erben RG, "Update on FGF23 and Klotho signaling", Mol Cell Endocrinol, 432, pp.56~65 (2016)[DOI]
- Slifer ZM, "The integral role of tight junction proteins in the repair of injured intestinal epithelium", Int J Mol Sci, 21, pp.972~ (2020)[DOI]
- Wongdee K, "Factors inhibiting intestinal calcium absorption : hormones and luminal factors that prevent excessive calcium uptake", J Physiol Sci, 69, pp.683~696 (2019)[DOI]
- Fujita H, "Tight junction proteins Claudin-2 and-12 are critical for Vitamin D-dependent Ca2+absorption between enterocytes", Mol Biol Cell, 19, pp.1912~1921 (2008)[DOI]
- Lee B, "Tight junction in the intestinal epithelium : Its association with diseases and regulation by phytochemicals", J Immunol Res, 2018, pp.2645465~ (2018)[DOI]