Effect of sodium butyrate on performance, immune status, microarchitecture of small intestinal mucosa and lymphoid organs in broiler chickens
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Objective: This study aimed to examine the effect of sodium butyrate (SB) on growth performance, immune status, organs weights, and microarchitecture of lymphoid organs and small intestine. Methods: A total of 120, 1-d-old broiler chicks were distributed into the following four treatment groups: corn-soy based basal diet (BD) without supplement (control), or the same BD supplemented with 0.1 g/kg zinc bacitracin (ZnB), 0.5 g/kg SB (SB-0.5), or 1.0 g/kg SB (SB-1), respectively. Six birds/group were killed on d-21 and d-35, and samples were collected. Results: Cell-mediated immune response at 48 h post-Phytohemagglutinin-P injection, and antibody titer against Newcastle disease vaccine and sheep red blood cells on d-35 was noted higher (p<0.05) in SB-1 compared to ZnB and control. Lower (p<0.05) feed conversion ratio (FCR) was attained by the supplemented groups. Thymus and spleen weighed more (p<0.05) in SB-1, and bursa registered more (p<0.05) weight in both SB groups compared to control. On d-21, areas of thymus medulla and spleen germinal centers were noted higher (p<0.05) in SB-1 group. The villus height and villus surface area increased (p<0.05) in duodenum and jejunum in both SB groups on d-21, and in SB-1 on d-35, respectively compared to ZnB and control. On d-21, number of goblet cells containing mucins of acidic nature increased (p<0.05) in all the segments of small intestines in SB-1 group compared to control, and on d-35 in ileum compared to other groups. Conclusion: In conclusion, SB improved growth performance and immunity as well as modulated morphology of lymphoid organs and gut mucosa in broiler chickens.
Abstract
Objective: This study aimed to examine the effect of sodium butyrate (SB) on growth performance, immune status, organs weights, and microarchitecture of lymphoid organs and small intestine. Methods: A total of 120, 1-d-old broiler chicks were distributed into the following four treatment groups: corn-soy based basal diet (BD) without supplement (control), or the same BD supplemented with 0.1 g/kg zinc bacitracin (ZnB), 0.5 g/kg SB (SB-0.5), or 1.0 g/kg SB (SB-1), respectively. Six birds/group were killed on d-21 and d-35, and samples were collected. Results: Cell-mediated immune response at 48 h post-Phytohemagglutinin-P injection, and antibody titer against Newcastle disease vaccine and sheep red blood cells on d-35 was noted higher (p<0.05) in SB-1 compared to ZnB and control. Lower (p<0.05) feed conversion ratio (FCR) was attained by the supplemented groups. Thymus and spleen weighed more (p<0.05) in SB-1, and bursa registered more (p<0.05) weight in both SB groups compared to control. On d-21, areas of thymus medulla and spleen germinal centers were noted higher (p<0.05) in SB-1 group. The villus height and villus surface area increased (p<0.05) in duodenum and jejunum in both SB groups on d-21, and in SB-1 on d-35, respectively compared to ZnB and control. On d-21, number of goblet cells containing mucins of acidic nature increased (p<0.05) in all the segments of small intestines in SB-1 group compared to control, and on d-35 in ileum compared to other groups. Conclusion: In conclusion, SB improved growth performance and immunity as well as modulated morphology of lymphoid organs and gut mucosa in broiler chickens.
참고문헌 (30)
- Kabploy K, "Effect of antibiotic growth promoters on anti-oxidative and anti-inflammatory activities in broiler chickens", Thai J Vet Med, 46, pp.89~95 (2016)
- Khan SH, "Recent advances in the role of organic acids in poultry nutrition", J Appl Anim Res, 44, pp.359~369 (2016)
- Moquet PCA, "Importance of release location on the mode of action of butyrate derivatives in the avian gastrointestinal tract", World’s Poult Sci J, 72, pp.61~80 (2016)
- Van Immerseel F, "Microencapsulated short-chain fatty acids in feed modify colonization and invasion early after infection with Salmonella Enteritidis in young chickens", Poult Sci, 83, pp.69~74 (2004)
- Qaisrani SN, "Diet structure, butyric acid, and fermentable carbohydrates influence growth performance, gut morphology, and cecal fermentation characteristics in broilers", Poult Sci, 94, pp.2152~2164 (2015)
- Chamba F, "Effect of partially protected sodium butyrate on performance, digestive organs, intestinal villi and E. coli development in broilers chickens", Int J Poult Sci, 13, pp.390~396 (2014)
- Fang CL, "Effects of sodium butyrate on growth performance, haematological and immunological characteristics of weanling piglets", J Anim Physiol Anim Nutr, 98, pp.680~685 (2014)
- Sunkara LT, "Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression", PLoS one, 6, pp.e27225~ (2011)
- Ahsan U, "Sodium butyrate in chicken nutrition: the dynamics of performance, gut microbiota, gut morphology, and immunity", World’s Poult Sci J, pp.265~275 (2016)
- M. A. Mohamed, "Effect of Using Organic Acids to Substitute Antibiotic Growth Promoters on Performance and Intestinal Microflora of Broilers", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 23(10), pp.1348~1353 (2010), 아세아·태평양축산학회
- Abdelqader A, "Effect of dietary butyric acid on performance, intestinal morphology, microflora composition and intestinal recovery of heat-stressed broilers", Livest Sci, 183, pp.78~83 (2016)
- Kiczorowska B, "The effect of dietary supplementation of the broiler chicken diet with Boswellia serrata resin on growth performance, digestibility, and gastrointestinal characteristics, morphology, and microbiota", Livest Sci, 191, pp.117~124 (2016)
- Corrier DE, "Comparison of phytohemagglutinin-induced cutaneous hypersensitivity reactions in the interdigital skin of broiler and layer chicks", Avian Dis, 34, pp.369~373 (1990)
- King DJ, "A comparison of the onset of protection induced by Newcastle disease virus strain B1 and a fowl poxvirus recombinant Newcastle disease vaccine to a viscerotropic velogenic Newcastle disease virus challenge", Avian Dis, 43, pp.745~755 (1999)
- Madej JP, "Effect of in ovo-delivered prebiotics and synbiotics on lymphoid-organs’ morphology in chickens", Poult Sci, 94, pp.1209~1219 (2015)
- Anwar S, "Effect of dietary supplementation of Catharanthus roseus on gross and micro-structures of selected internal organs of broilers", M.Phil, pp.2013
- Sikandar A, "Histopathological and serological studies on paratuberculosis in cattle and buffaloes", Pak Vet J, 32, pp.547~551 (2012)
- Bancroft JD, "Theory and practice of histological techniques" (2008), Elsevier Health Sciences
- Ashraf S, "Effect of dietary supplementation of prebiotics and probiotics on intestinal microarchitecture in broilers reared under cyclic heat stress", J Anim Physiol Anim Nutr, 1, pp.68~73 (2013)
- Rieger J, "Intraepithelial lymphocyte numbers and histomorphological parameters in the porcine gut after Enterococcus faecium NCIMB 10415 feeding in a Salmonella Typhimurium challenge", Vet Immunol Immunopathol, 164, pp.40~50 (2015)
- Dehghani-Tafti N, "Effect of supplemental organic acids on performance, carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels", Anim Feed Sci Technol, 211, pp.109~116 (2016)
- Zhang WH, "Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens", Br Poult Sci, 52, pp.292~301 (2011)
- Schrank CS, "Immune response of Mallard Ducks treated with immunosuppressive agents: antibody response to erythrocytes and in vivo response to phytohemagglutinin-P", J Wildlife Dis, 26, pp.307~315 (1990)
- Geng T, "Screening for genes involved in antibody response to sheep red blood cells in the chicken, Gallus gallus", Poult Sci, 94, pp.2099~2107 (2015)
- El-Abasy M, "Adjuvant effects of sugar cane extracts (SCE) in chickens", J Vet Med Sci, 65, pp.117~119 (2003)
- Park J, "Short-chain fatty acids induce both effector and regulatory T cells by suppression of histone deacetylases and regulation of the mTOR–S6K pathway", Mucosal Immunol, 8, pp.80~93 (2015)
- Zhou ZY, "Effect of butyrate on immune response of a chicken macrophage cell line", Vet Immunol Immunopathol, 162, pp.24~32 (2014)
- Eshak MG, "The efficacy of Na-butyrate encapsulated in palm fat on performance of broilers infected with necrotic enteritis with gene expression analysis", Vet World, 9, pp.450~457 (2016)
- Chang PV, "The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition", Proc Natl Acad Sci, 111, pp.2247~2252 (2014)
- Majidi-Mosleh A, "Influence of in ovo inoculation of probiotic strains on the jejunal goblet cell counts and morphometry in peri- and post-hatching chicks", Kafkas Univ Vet Fak Derg, 23, pp.169~172 (2017)
