Gallic acid caused cultured mice TM4 Sertoli cells apoptosis and necrosis
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Objective: The study was designed to determine the cytotoxic effect of gallic acid (GA), obtained by the hydrolysis of tannins, on mice TM4 Sertoli cells apoptosis. Methods: In the present study, non-tumorigenic mice TM4 Sertoli cells were treated with different concentrations of GA for 24 h. After treatment, cell viability was evaluated using WST-1, mitochondrial dysfunction, cells apoptosis and necrosis was detected using JC-1, Hoechst 33342 and propidium iodide staining. The expression levels of Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 were also detected by quantitative real-time polymerase chain reaction and Western-blotting. Results: The results showed that 20 to 400 μM GA inhibited viability of TM4 Sertoli cells in a dose-dependent manner. Treatment with 400 μM GA significantly inhibited PCNA and Cyclin B1 expression, however up-regulated BAX and Caspase-3 expression, caused mitochondrial membrane depolarization, activated Caspase-3, and induced DNA damage, thus, markedly increased the numbers of dead cells. Conclusion: Our findings showed that GA could disrupt mitochondrial function and caused TM4 cells to undergo apoptosis and necrosis.
Abstract
Objective: The study was designed to determine the cytotoxic effect of gallic acid (GA), obtained by the hydrolysis of tannins, on mice TM4 Sertoli cells apoptosis. Methods: In the present study, non-tumorigenic mice TM4 Sertoli cells were treated with different concentrations of GA for 24 h. After treatment, cell viability was evaluated using WST-1, mitochondrial dysfunction, cells apoptosis and necrosis was detected using JC-1, Hoechst 33342 and propidium iodide staining. The expression levels of Cyclin B1, proliferating cell nuclear antigen (PCNA), Bcl-2-associated X protein (BAX), and Caspase-3 were also detected by quantitative real-time polymerase chain reaction and Western-blotting. Results: The results showed that 20 to 400 μM GA inhibited viability of TM4 Sertoli cells in a dose-dependent manner. Treatment with 400 μM GA significantly inhibited PCNA and Cyclin B1 expression, however up-regulated BAX and Caspase-3 expression, caused mitochondrial membrane depolarization, activated Caspase-3, and induced DNA damage, thus, markedly increased the numbers of dead cells. Conclusion: Our findings showed that GA could disrupt mitochondrial function and caused TM4 cells to undergo apoptosis and necrosis.
참고문헌 (30)
- Fan L, "Studies on the determination of Tannin content in sorghum", J Chinese Cereals Oil Assoc, 16, pp.13~17 (2001)
- Yang JC, "Comparison of cold resistance and nutritional quality of six tropical and subtropical leguminous forages during overwintering period", Partacultural Sci, 34, pp.794~801 (2017)
- Wang Z, "Detecting on polyphenol and tannin contents in different wine grapes form Penglai region", Sino Overseas Grapevine Wine, pp.35~37 (2015)
- Halvorson JJ, "Effects of dietary tannins on total and extractable nutrients from manure", J Anim Sci, 95, pp.3654~3665 (2017)
- Vissers AM, "Laminaria digitata phlorotannins decrease protein degradation and methanogenesis during in vitro ruminal fermentation", J Sci Food Agric, 98, pp.3644~3650 (2018)
- Kronberg SL, "Condensed tannin in drinking water reduces greenhouse gas precursor urea in sheep and cattle urine", Rangeland Ecol Manag, 64, pp.543~547 (2011)
- Ye YL, "Dynamic changes in amino acids, catechins, caffeine and gallic acid in green tea during withering", J Food Compost Anal, 66, pp.98~108 (2018)
- Jiang J, "Natural antioxidants as food and feed additives to promote health benefits and quality of meat products : a review", Meat Sci, 120, pp.107~117 (2016)
- You BR, "Gallic acid-induced lung cancer cell death is related to glutathione depletion as well as reactive oxygen species increase", Toxicol In Vitro, 24, pp.1356~1362 (2010)
- Abarikwu SO, "Combined administration of curcumin and gallic acid inhibits gallic acid-induced suppression of steroidogenesis, sperm output, antioxidant defenses and inflammatory responsive genes", J Steroid Biochem Mol Biol, 143, pp.49~60 (2014)
- Mather JP, "Establishment and characterization of two distinct mouse testicular epithelial cell lines", Biol Reprod, 23, pp.243~252 (1980)
- Park WH, "Cytoprotective effect of Panax ginseng on gallic acid-induced toxicity in TM3 mouse Leydig cells", Fitoterapia, 78, pp.577~579 (2007)
- Xie YY, "DNA interaction, cytotoxicity, apoptotic activity, cell cycle arrest, reactive oxygen species and mitochondrial membrane potential assay induced by ruthenium(II)polypyridyl complexes", Inorganica Chim Acta, 405, pp.228~234 (2013)
- Begum P, "Induction of cell death by graphene in Arabidopsis thaliana(Columbia ecotype)T87 cell suspensions", J Hazard Mater, 260, pp.1032~1041 (2013)
- Kroon PA, "Bioavailability and bioefficacy of polyphenols in humans. Ι. Review of 97 bioavailablity studies", Am J Clin Nutra, 81(Suppl 1), pp.230S~242S (2006)
- You BR, "Gallic acid-induced lung cancer cell death is accompanied by ROS increase and glutathione depletion", Mol Cell Biochem, 357, pp.295~303 (2011)
- Coutinho EM, "Gossypol : a contraceptive for men", Contraception, 65, pp.259~263 (2002)
- Lamas CA, "Grape juice concentrate(G8000((R)))intake mitigates testicular morphological and ultrastructural damage following cadmium intoxication", Int J Exp Pathol, 96, pp.301~310 (2015)
- Shehab NG, "Phenolic Profiling and evaluation of contraceptive effect of the ethanolic extract of salsola imbricata forssk. in male albino rats", Evid-Based Complement Alternat Med, 2014 (2014)
- Hajhosseini L, "Effect of rosmarinic acid on Sertoli cells apoptosis and serum antioxidant levels in rats after exposure to electromagnetic fields", Afr J Tradit Complement Altern Med, 10, pp.477~480 (2013)
- Huang JF, "Antiproliferative effect of azadirachtin A on Spodoptera litura Sl-1 cell line through cell cycle arrest and apoptosis induced by up-regulation of p53", Pestic Biochem Physiol, 99, pp.16~24 (2011)
- Chimento A, "17β-Estradiol activates GPER-and ESR1-dependent pathways inducing apoptosis in GC-2 cells, a mouse spermatocyte-derived cell line", Mol Cell Endocrinol, 355, pp.49~59 (2012)
- Zhang G, "Dioscin suppresses hepatocellular carcinoma tumor growth by inducing apoptosis and regulation of TP53, BAX, BCL2 and cleaved CASP3", Phytomedicine, 23, pp.1329~1336 (2016)
- Hsu JD, "Gallic acid induces G2", M phase arrest of breast cancer cell MCF-7 through stabilization of p27(Kip1)attributed to disruption of p27(Kip1), J Agric Food Chem, pp.2011, 59[DOI]
- Ji BC, "Gallic acid induces apoptosis via caspase-3 and mitochondrion-dependent pathways in vitro and suppresses lung xenograft tumor growth in vivo", J Agric Food Chem, 57, pp.7596~7604 (2009)
- Tan S, "Gallic acid induces mitotic catastrophe and inhibits centrosomal clustering in HeLa cells", Toxicol In Vitro, 30, pp.506~513 (2015)
- Czabotar PE, "Control of apoptosis by the BCL-2 protein family : implications for physiology and therapy", Nat Rev Mol Cell Biol, 15, pp.49~63 (2014)
- Owens TW, "Role for X-linked Inhibitor of apoptosis protein upstream of mitochondrial permeabilization", J Biol Chem, 285, pp.1081~1088 (2010)
- Cepero E, "Caspase-9 and effector caspases have sequential and distinct effects on mitochondria", Oncogene, 24, pp.6354~6366 (2005)
- Park W, "Cytotoxic effect of gallic acid on testicular cell lines with increasing H2O2 level in GC-1 spg cells", Toxicol In Vitro, 22, pp.159~163 (2008)