Tannic acid-induced apoptosis in FaDu hypopharyngeal squamous cell carcinoma
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Tannic acid (TA) is a water-soluble polyphenol compound found in various herbal plants. We investigated the chemopreventive effects of TA on FaDu hypopharyngeal squamous carcinoma cells. In an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, TA showed dose-dependent cytotoxicity with a half maximal inhibitory concentration (IC50) of 50 µM. Cell cycle analysis and immunofluorescence imaging demonstrated that under low-dose (25 µM) treatment, FaDu cells were arrested in G2/M phase, and as the dose of TA was increased, apoptosis was induced with the increase of cell population at sub-G1 phase. The expressions of various cyclins, including cyclin D1 and cyclin-dependent kinases (CDK-1 and CDK-2), were down-regulated at low doses of TA, whereas apoptotic effectors such as cleaved caspase 3, cleaved caspase 7, and poly (ADP-ribose) polymerase (PARP) were expressed in a dose-dependent manner in Western blotting. In addition, TA-induced apoptosis of FaDu cells might be mediated by the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase pathway, with the upregulation of p-AKT/p-PKB (phosphorylated protein kinase B) and p-ERK. Overall, our data support the hypothesis that TA is a potential candidate agent for the treatment of hypopharyngeal cancer.
Abstract
Tannic acid (TA) is a water-soluble polyphenol compound found in various herbal plants. We investigated the chemopreventive effects of TA on FaDu hypopharyngeal squamous carcinoma cells. In an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, TA showed dose-dependent cytotoxicity with a half maximal inhibitory concentration (IC50) of 50 µM. Cell cycle analysis and immunofluorescence imaging demonstrated that under low-dose (25 µM) treatment, FaDu cells were arrested in G2/M phase, and as the dose of TA was increased, apoptosis was induced with the increase of cell population at sub-G1 phase. The expressions of various cyclins, including cyclin D1 and cyclin-dependent kinases (CDK-1 and CDK-2), were down-regulated at low doses of TA, whereas apoptotic effectors such as cleaved caspase 3, cleaved caspase 7, and poly (ADP-ribose) polymerase (PARP) were expressed in a dose-dependent manner in Western blotting. In addition, TA-induced apoptosis of FaDu cells might be mediated by the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase pathway, with the upregulation of p-AKT/p-PKB (phosphorylated protein kinase B) and p-ERK. Overall, our data support the hypothesis that TA is a potential candidate agent for the treatment of hypopharyngeal cancer.
참고문헌 (29)
- R J Sanderson, "Squamous cell carcinomas of the head and neck * Commentary: Head and neck carcinomas in the developing world", BMJ, 325(7368), pp.822~827 (2002), BMJ[DOI]
- Lindsey A. Torre, "Global cancer statistics, 2012", CA: A Cancer Journal for Clinicians, 65(2), pp.87~108 (2015), Wiley[DOI]
- Ewa Majchrzak, "Oral cavity and oropharyngeal squamous cell carcinoma in young adults: a review of the literature", Radiology and Oncology, 48(1), pp.1~10 (2014), Walter de Gruyter GmbH[DOI]
- Negri E, "Attributable risk for oral cancer in northern Italy", Cancer Epidemiol Biomarkers Prev, 2, pp.189~193 (1993)
- IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, "Tobacco smoke and involuntary smoking", IARC Monogr Eval Carcinog Risks Hum, 83, pp.1~1438 (2004)
- Assuntina G. Sacco, "Current Treatment Options for Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma", Journal of Clinical Oncology, 33(29), pp.3305~3313 (2015), American Society of Clinical Oncology (ASCO)[DOI]
- Jean-Pierre Pignon, "Meta-analysis of chemotherapy in head and neck cancer (MACH-NC): An update on 93 randomised trials and 17,346 patients", Radiotherapy and Oncology, 92(1), pp.4~14 (2009), Elsevier BV[DOI]
- Y Suh, "Clinical update on cancer: molecular oncology of head and neck cancer", Cell Death & Disease, 5(1), pp.e1018~e1018 (2014), Springer Science and Business Media LLC[DOI]
- Nathaniel D. Wycliffe, "Hypopharyngeal Cancer", Topics in Magnetic Resonance Imaging, 18(4), pp.243~258 (2007), Ovid Technologies (Wolters Kluwer Health)[DOI]
- Frutos P, "Review. Tannins and ruminant nutrition", Span J Agric Res, 2, pp.191~202 (2004)[DOI]
- Katalin Erdèlyi, "Gallotannin Inhibits the Expression of Chemokines and Inflammatory Cytokines in A549 Cells", Molecular Pharmacology, 68(3), pp.895~904 (2005), American Society for Pharmacology & Experimental Therapeutics (ASPET)[DOI]
- Nelofer S Khan, "Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA", Chemico-Biological Interactions, 125(3), pp.177~189 (2000), Elsevier BV[DOI]
- Oday O. Hamiza, "Amelioration of 1,2 Dimethylhydrazine (DMH) Induced Colon Oxidative Stress, Inflammation and Tumor Promotion Response by Tannic Acid in Wistar Rats", Asian Pacific Journal of Cancer Prevention, 13(9), pp.4393~4402 (2012), Asian Pacific Organization for Cancer Prevention[DOI]
- Hikoya Hayatsu, "Dietary inhibitors of mutagenesis and carcinogenesis", Mutation Research, 1988, pp.202(2), 429~446[DOI]
- Nikita Savelyev, "Comprehensive analysis of telomerase inhibition by gallotannin", Oncotarget, 9(27), pp.18712~18719 (2018), Impact Journals, LLC[DOI]
- Prashanth Nagesh, "Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer", Cancers, 10(3), pp.68~ (2018), MDPI AG[DOI]
- Lauren G. Jordan, "HER2+ breast cancer cells undergo apoptosis upon exposure to tannic acid released from remodeled cross-linked collagen type I", Journal of Biomedical Materials Research Part A, 106(1), pp.26~32 (2018), Wiley[DOI]
- Pramod Darvin, "Tannic acid inhibits EGFR", STAT1, STAT1 signalling axis in breast cancer cells, pp.Journal of Cellular and Molecular Medicine, 2017[DOI]
- Jian Zhang, "Tannic acid mediated induction of apoptosis in human glioma Hs 683 cells", Oncology Letters, 15, pp.6845~6850 (2018), Spandidos Publications[DOI]
- Małgorzata Zielińska-Przyjemska, "The effect of resveratrol, its naturally occurring derivatives and tannic acid on the induction of cell cycle arrest and apoptosis in rat C6 and human T98G glioma cell lines", Toxicology in Vitro, 43, pp.69~75 (2017), Elsevier BV[DOI]
- PRAMOD DARVIN, "Tannic acid inhibits the Jak2", STAT3 pathway and induces G1, International Journal of Oncology, pp.2015, 47(3)[DOI]
- J. Alan Diehl, "Cycling to Cancer with Cyclin D1", Cancer Biology & Therapy, 1(3), pp.226~231 (2014), Informa UK Limited[DOI]
- Guy Mordret, "MAP kinase kinase: A node connecting multiple pathways", Biology of the Cell, 79(3), pp.193~207 (1993), Wiley[DOI]
- Yu Sun, "Signaling pathway of MAPK", ERK in cell proliferation, differentiation, migration, senescence and apoptosis, 2015, pp.35(6), 600~604[DOI]
- T F Franke, "PI3K", Akt: getting it right matters, 2008, pp.27(50), 6473~6488[DOI]
- Prashanth T. Bhaskar, "The Two TORCs and Akt", Developmental Cell, 12(4), pp.487~502 (2007), Elsevier BV[DOI]
- S. Maddika, "Akt-mediated phosphorylation of CDK2 regulates its dual role in cell cycle progression and apoptosis", Journal of Cell Science, 121(7), pp.979~988 (2008), The Company of Biologists[DOI]
- Mahandranauth A. Chetram, "ROS-mediated activation of AKT induces apoptosis via pVHL in prostate cancer cells", Molecular and Cellular Biochemistry, 376(1-2), pp.63~71 (2013), Springer Science and Business Media LLC[DOI]
- Marianne Schmidt, "The influence of Osmunda regalis root extract on head and neck cancer cell proliferation, invasion and gene expression", BMC Complementary and Alternative Medicine, 17(1), pp.518~ (2017), Springer Science and Business Media LLC[DOI]