본문 바로가기
KERD
논문KCI 등재

Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

초록

Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells, somatic cell nuclear transfer, cryopreservation, and nonsurgical embryo transfer. The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.

Abstract

Gene editing (GE) offers a new breeding technique (NBT) of sustainable value to animal agriculture. There are 3 GE working sites covering 5 feasible pathways to generate GE pigs along with the crucial intervals of GE/genotyping, microinjection/electroporation, induced pluripotent stem cells, somatic cell nuclear transfer, cryopreservation, and nonsurgical embryo transfer. The extension of NBT in the new era of pig breeding depends on the synergistic effect of GE and reproductive biotechnologies; the outcome relies not only on scientific due diligence and operational excellence but also on the feasibility of application on farms to improve sustainability.

참고문헌 (99)

  1. Tu Ching-Fu, "The application of new breeding technology based on gene editing in pig industry - A review", Animal Bioscience, 35(6), pp.791~803 (2022), 아세아·태평양축산학회[DOI]
  2. Tanihara F, "Current status of the application of gene editing in pigs", J Reprod Dev, 67, pp.177~187 (2021)[DOI]
  3. Zhang J, "The advancements, challenges, and future implications of the CRISPR", Cas9system in swine research, 2021, pp.48, 347~360
  4. Bétermier M, "Is non-homologous end-joining really an inherently error-prone process?", PLoS Genet, 10, pp.e1004086~ (2014)[DOI]
  5. Bennett EP, "INDEL detection, the 'Achilles heel' of precise genome editing : a survey of methods for accurate profiling of gene editing induced indels", Nucleic Acids Res, 48, pp.11958~11981 (2020)[DOI]
  6. Chuang CK, "Points of view on the tools for genome", gene editing, 2021, pp.22, 9872~
  7. Chuang CK, "Porcine type III RNA polymerase III promoters for short hairpin RNA expression", Anim Biotechnol, 20, pp.34~39 (2009)[DOI]
  8. Christou-Kent M, "Diversity of RNA-binding proteins modulating posttranscriptional regulation of protein expression in the maturing mammalian oocyte", Cells, 9, pp.662~ (2020)[DOI]
  9. Li L, "Maternal control of early mouse development", Development, 137, pp.859~870 (2010)[DOI]
  10. Chuang CK, "Generation of GGTA1mutant pigs by direct pronuclear microinjection of CRISPR", Cas9 plasmid vectors, 2017, pp.28, 174~181
  11. Tu CF, "Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR", Cas9 to nullify N-glycolylneuraminic acid expression, 2019, pp.14, e0217236~
  12. Shao-Wen Hung, "Activated macrophages of CD 163 gene edited pigs generated by direct cytoplasmic microinjection with CRISPR gRNA", Cas9 mRNA are resistant to PRRS virus assault, 2022, pp.34(9), 4196~4209[DOI]
  13. Tanihara F, "Generation of PDX-1mutant porcine blastocysts by introducing CRISPR", Cas9-system into porcine zygotes via electroporation, 2019, pp.90, 55~61
  14. Tanihara F, "Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR", Cas9 system into in vitro-fertilized zygotes, 2020, pp.20, 40~
  15. Tanihara F, "Generation of CD163-edited pig via electroporation of the CRISPR", Cas9 system into porcine in vitro-fertilized zygotes, 2021, pp.32, 147~154
  16. Ren C, "Strategies for the enrichment and selection of genetically modified cells", Trends Biotechnol, 37, pp.56~71 (2019)[DOI]
  17. Takahashi K, "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors", Cell, 126, pp.663~676 (2006)[DOI]
  18. Esteban MA, "Generation of induced pluripotent stem cell lines from Tibetan miniature pig", J Biol Chem, 284, pp.17634~17640 (2009)[DOI]
  19. Ezashi T, "Derivation of induced pluripotent stem cells from pig somatic cells", Proc Natl Acad Sci USA, 106, pp.10993~10998 (2009)[DOI]
  20. Wu Z, "Generation of pig induced pluripotent stem cells with a drug-inducible system", J Mol Cell Biol, 1, pp.46~54 (2009)[DOI]
  21. West FD, "Porcine induced pluripotent stem cells produce chimeric offspring", Stem Cells Dev, 19, pp.1211~1220 (2010)
  22. West FD, "Brief report : chimeric pigs produced from induced pluripotent stem cells demonstrate germline transmission and no evidence of tumor formation in young pigs", Stem Cells, 29, pp.1640~1643 (2011)[DOI]
  23. Liu K, "Generation of porcine-induced pluripotent stem cells by using OCT4 and KLF4 porcine factors", Cell Reprogram, 14, pp.505~513 (2012)[DOI]
  24. Du X, "Barriers for deriving transgene-free pig iPS cells with episomal vectors", Stem Cells, 33, pp.3228~3238 (2015)[DOI]
  25. Chen CH, "Germline competent pluripotent mouse stem cells generated by plasmid vectors", Anim Biotechnol, 27, pp.157~165 (2016)[DOI]
  26. Kim JY, "Review of the current trends in clinical trials involving induced pluripotent stem cells", Stem Cell Rev Rep, 18, pp.142~154 (2022)[DOI]
  27. Howden SE, "Simultaneous reprogramming and gene correction of patient fibroblasts", Stem Cell Rep, 5, pp.1109~1118 (2015)[DOI]
  28. Cheng D, "Porcine induced pluripotent stem cells require LIF and maintain their developmental potential in early stage of embryos", PLoS One, 7, pp.e51778~ (2012)[DOI]
  29. Yuan Y, "Cell cycle synchronization of leukemia inhibitory factor(LIF)-dependent porcine-induced pluripotent stem cells and the generation of cloned embryos", Cell Cycle, 13, pp.1265~1276 (2014)[DOI]
  30. Kim E, "Putative embryonic stem cells derived from porcine cloned blastocysts using induced pluripotent stem cells as donors", Theriogenology, 85, pp.601~616 (2016)[DOI]
  31. Fan N, "Piglets cloned from induced pluripotent stem cells", Cell Res, 23, pp.162~166 (2013)[DOI]
  32. Yu D, "Silencing of retrotransposonderived imprinted gene RTL1 is the main cause for postimplantational failures in mammalian cloning", Proc Natl Acad Sci, 115, pp.E11071~ (2018)[DOI]
  33. Wilmut I, "Viable offspring derived from fetal and adult mammalian cells", Nature, 385, pp.810~813 (1997)[DOI]
  34. Polejaeva IA, "Cloned pigs produced by nuclear transfer from adult somatic cells", Nature, 407, pp.86~90 (2000)[DOI]
  35. Betthauser J, "Production of cloned pigs from in vitro systems", Nat Biotechnol, 18, pp.1055~1059 (2000)[DOI]
  36. Onishi A, "Pig cloning by microinjection of fetal fibroblast nuclei", Science, 289, pp.1188~1190 (2000)[DOI]
  37. Hua Z, "Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig", Pol J Vet Sci, 19, pp.205~212 (2016)[DOI]
  38. Richter A, "Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig", BMC Biotechnol, 12, pp.84~ (2012)[DOI]
  39. Fahrudin M, "Development to the blastocyst stage of porcine somatic cell nuclear transfer embryos reconstructed by the fusion of cumulus cells and cytoplasts prepared by gradient centrifugation", Cloning Stem Cells, 9, pp.216~228 (2007)[DOI]
  40. Li X, "Aging adult porcine fibroblasts can support nuclear transfer and transcription factor-mediated reprogramming", Anim Sci J, 89, pp.289~297 (2018)[DOI]
  41. Li Z, "Bone marrow mesenchymal stem cells are an attractive donor cell type for production of cloned pigs as well as genetically modified cloned pigs by somatic cell nuclear transfer", Cell Reprogram, 15, pp.459~470 (2013)[DOI]
  42. Park CH, "Extraembryonic endoderm(XEN)cells capable of contributing to embryonic chimeras established from pig embryos", Stem Cell Rep, 16, pp.212~223 (2021)[DOI]
  43. Dang-Nguyen TQ, "Combined refinements to somatic cell nuclear transfer methods improve porcine embryo development", J Reprod Dev, 66, pp.281~286 (2020)[DOI]
  44. Jiao D, "Improving porcine SCNT efficiency by selecting donor cells size", Cell Cycle, 20, pp.2264~2277 (2021)[DOI]
  45. Yin XJ, "Production of cloned pigs from adult somatic cells by chemically assisted removal of maternal chromosomes", Biol Reprod, 67, pp.442~446 (2002)[DOI]
  46. Du Y, "Piglets born from handmade cloning, an innovative cloning method without micromanipulation", Theriogenology, 68, pp.1104~1110 (2007)[DOI]
  47. Betthauser J, "Production of cloned pigs from in vitro systems", Nat Biotechnol, 18, pp.1055~1059 (2000)[DOI]
  48. Du Y, "Piglets born from handmade cloning, an innovative cloning method without micromanipulation", Theriogenology, 68, pp.1104~1110 (2007)[DOI]
  49. de Macedo MP, "A fast and reliable protocol for activation of porcine oocytes", Theriogenology, 123, pp.22~29 (2019)[DOI]
  50. Lee K, "Pig oocyte activation using a Zn2+ chelator, TPEN", Theriogenology, 84, pp.1024~1032 (2015)[DOI]
  51. Wang X, "Epigenetic reprogramming during somatic cell nuclear transfer : recent progress and future directions", Front Genet, 11, pp.205~ (2020)[DOI]
  52. Zeng Y, "DNA methylation reprogramming during mammalian development", Genes, 10, pp.257~ (2019)[DOI]
  53. Ju S, "Analysis of apoptosis and methyltransferase mRNA expression in porcine cloned embryos cultured in vitro", J Assist Reprod Genet, 27, pp.49~59 (2010)[DOI]
  54. Hye-Bin Park, "Endoplasmic Reticulum (ER) Stress Inhibitor or Antioxidant Treatments during Micromanipulation Can Inhibit Both ER and Oxidative Stresses in Porcine SCNT Embryos", 발생과 생식, 24(1), pp.31~41 (2020), 한국발생생물학회
  55. Park J, "Disruption of mitochondrionto-nucleus interaction in deceased cloned piglets", PLoS One, 10, pp.e0129378~ (2015)[DOI]
  56. Srirattana K, "Strategies to improve the efficiency of somatic cell nuclear transfer", Int J Mol Sci, 23, pp.1969~ (2022)[DOI]
  57. Chen Z, "Role of mammalian DNA methyltransferases in development", Annu Rev Biochem, 89, pp.135~158 (2019)[DOI]
  58. Gu TP, "The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes", Nature, 477, pp.606~610 (2011)[DOI]
  59. Inoue A, "Replication-dependent loss of 5-hydroxymethylcytosine in mouse preimplantation embryos", Science, 334, pp.194~ (2011)[DOI]
  60. Huang Y, "Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos", Biochem Biophys Res Commun, 411, pp.397~401 (2011)[DOI]
  61. Kere M, "Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation", J Reprod Dev, 59, pp.78~84 (2013)[DOI]
  62. Yin R, "Ascorbic acid enhances Tetmediated 5-methylcytosine oxidation and promotes DNA demethylation in mammals", J Am Chem Soc, 135, pp.10396~10403 (2013)[DOI]
  63. Minghui Zhao, "Ascorbic acid increases demethylation in somatic cell nuclear transfer embryos of the pig (Sus scrofa)", Animal Bioscience, 30(7), pp.944~949 (2017), 아세아·태평양축산학회[DOI]
  64. Lee K, "Dynamics of TET family expression in porcine preimplantation embryos is related to zygotic genome activation and required for the maintenance of NANOG", Dev Biol, 386, pp.86~95 (2014)[DOI]
  65. Guo Z, "Effects of trichostatin A on pig SCNT blastocyst formation rate and cell number : A metaanalysis", Res Vet Sci, 117, pp.161~166 (2018)[DOI]
  66. Zhao J, "Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer", Biol Reprod, 81, pp.525~530 (2009)[DOI]
  67. Zhao J, "Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic cell nuclear transfer porcine embryos", Cell Reprogram, 12, pp.75~83 (2010)[DOI]
  68. Liang S, "Scriptaid treatment decreases DNA methyltransferase 1 expression by induction of microRNA-152 expression in porcine somatic cell nuclear transfer embryos", PLoS One, 10, pp.e0134567~ (2015)[DOI]
  69. Jeong PS, "Chaetocin improves pig cloning efficiency by enhancing epigenetic reprogramming and autophagic activity", Int J Mol Sci, 21, pp.4836~ (2020)[DOI]
  70. Kobayashi W, "Structural transition of the nucleosome during chromatin remodeling and transcription", Curr Opin Struct Biol, 59, pp.107~114 (2019)[DOI]
  71. Antony J, "Transient JMJD2Bmediated reduction of H3K9me3 levels improves reprogramming of embryonic stem cells into cloned embryos", Mol Cell Biol, 33, pp.974~983 (2013)[DOI]
  72. Matoba S, "Embryonic development following somatic cell nuclear transfer impeded by persisting histone methylation", Cell, 159, pp.884~895 (2014)[DOI]
  73. Chung YG, "Histone demethylase expression enhances human somatic cell nuclear transfer effciency and promotes derivation of pluripotent stem cells", Cell Stem Cell, 17, pp.758~766 (2015)[DOI]
  74. Liu Z, "Cloning of macaque monkeys by somatic cell nuclear transfer", Cell, 172, pp.881~887 (2018)[DOI]
  75. Weng XG, "Improvement in the in vitro development of cloned pig embryos after kdm4a overexpression and an H3K9me3 methyltransferase inhibitor treatment", Theriogenology, 146, pp.162~170 (2020)[DOI]
  76. Greiner D, "Identification of a specific inhibitor of the histone methyltransferase SU(VAR)3-9", Nat Chem Biol, 1, pp.143~145 (2005)[DOI]
  77. Jeong PS, "Chaetocin improves pig cloning efficiency by enhancing epigenetic reprogramming and autophagic activity", Int J Mol Sci, 21, pp.4836~ (2020)[DOI]
  78. Jeong PS, "Combined chaetocin", trichostatin A treatment improves the epigenetic modification and developmental competence of porcine somatic cell nuclear transfer embryos, 2021, pp.9, 709574~
  79. Yoshino J, "Cryopreservation of porcine blastocysts by vitrification", Cryobiology, 30, pp.413~422 (1993)[DOI]
  80. Katayama KP, "High survival rate of vitrified human oocytes results in clinical pregnancy", Fertil Steril, 80, pp.223~224 (2003)[DOI]
  81. Esaki R, "Cryopreservation of porcine embryos derived from in vitro-matured oocytes", Biol Reprod, 71, pp.432~437 (2004)[DOI]
  82. Cuello C, "Vitrification of porcine embryos at various developmental stages using different ultra-rapid cooling procedures", Theriogenology, 62, pp.353~361 (2004)[DOI]
  83. Cuello C, "Effect of the cryoprotectant concentration on the in vitro embryo development and cell proliferation of OPS-vitrified porcine blastocysts", Cryobiology, 56, pp.189~194 (2008)[DOI]
  84. Du Y, "Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transfer", Cloning Stem Cells, 9, pp.469~476 (2007)[DOI]
  85. Nakano K, "Cloned porcine embryos can maintain developmental ability after cryopreservation at the morula stage", J Reprod Dev, 57, pp.312~316 (2011)[DOI]
  86. Jia B, "Successful vitrification of earlystage porcine cloned embryos", Cryobiology, 97, pp.53~59 (2020)[DOI]
  87. Du X, "Cryopreservation of porcine embryos : recent updates and progress", Biopreserv Biobank, 19, pp.210~218 (2021)[DOI]
  88. Kamoshita M, "Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated epsilon-poly-L-lysine", PLoS One, 12, pp.e0176711~ (2017)[DOI]
  89. Li J, "Technical note : porcine non-surgical embryo transfer", J Anim Sci, 74, pp.2263~2268 (1996)[DOI]
  90. Suzuki C, "Birth of piglets through the non-surgical transfer of blastocysts produced in vitro", J Reprod Dev, 50, pp.487~491 (2004)[DOI]
  91. Ducro-Steverink DW, "Reproduction results and offspring performance after non-surgical embryo transfer in pigs", Theriogenology, 62, pp.522~531 (2004)[DOI]
  92. Cuello C, "Piglets born after non-surgical deep intrauterine transfer of vitrified blastocysts in gilts", Anim Reprod Sci, 85, pp.275~286 (2005)[DOI]
  93. Yoshioka K, "Production of piglets from in vitro-produced embryos following non-surgical transfer", Anim Reprod Sci, 131, pp.23~29 (2012)[DOI]
  94. Gomis J, "Non-surgical deep intrauterine transfer of superfine open pulled straw(SOPS)-vitrified porcine embryos-evaluation of critical steps of the procedure", Theriogenology, 78, pp.1339~1349 (2012)[DOI]
  95. Martinez EA, "The recipients’parity does not influence their reproductive performance following non-surgical deep uterine porcine embryo transfer", Reprod Dom Anim, 51, pp.123~129 (2016)[DOI]
  96. Angel MA, "An earlier uterine environment favors the in vivo development of fresh pig morulae and blastocysts transferred by a nonsurgical deep-uterine method", J Reprod Dev, 60, pp.371~376 (2014)[DOI]
  97. Tajima S, "Insemination of recipient sows improves the survival to term of vitrified and warmed porcine expanded blastocysts transferred non-surgically", Anim Sci J, 91, pp.e13453~ (2020)[DOI]
  98. Hirayama Y, "Non-surgical transfer of vitrified porcine embryos using a catheter designed for a proximal site of the uterus", Anim Sci J, 91, pp.e13457~ (2020)[DOI]
  99. Tajima S, "Piglet production by non-surgical transfer of vitrified embryos, transported to commercial swine farms and warmed on site", Anim Sci J, 91, pp.e13476~ (2020)[DOI]

관련 논문AI추천

이 간행물의 다른 논문 (21건)