본문 바로가기
KERD
논문KCI 등재

Effect of in ovo feeding of γ-aminobutyric acid combined with embryonic thermal manipulation on hatchability, growth, and hepatic gene expression in broilers

1

조회

0

다운로드

1

인용

0

참고문헌

키워드

초록

Objective: This study investigated the effects of in ovo feeding of γ-aminobutyric acid (GABA) and embryonic thermal manipulation (ETM) on growth performance, organ indices, plasma biochemical parameters, hepatic antioxidant levels, and expression of lipid metabolism-related genes in broilers. Methods: Two hundred and fifty eggs were assigned to one of four treatments: control eggs incubated under standard conditions (CON); eggs that received an in ovo injection of 10% GABA on day 17.5 of incubation (G10); thermally manipulated eggs between days 10 and 18 of incubation at 39.6°C for 6 h daily (TM); and eggs that received both treatments during incubation (G10+TM). After 28 days of rearing, five birds per treatment were selected for blood and organ sampling. Results: No differences were found in hatchability or growth parameters among different treatment groups. Hepatic gene expression of catalase (CAT) and glutathione peroxidase 1 (GPx1) was upregulated (p = 0.046 and p = 0.006, respectively) in the G10+TM group, while that of nuclear factor erythroid 2–related factor 2 (NRF2) was upregulated (p = 0.039) in the G10 group. In addition, the relative gene expression of NADPH oxidase 1 (NOX1) was significantly lower (p = 0.007) in all treatment groups than that in the CON group. Hepatic fatty acid synthase (FAS) levels and average daily feed intake (ADFI) of last week showed a positive correlation (r = 0.50, p = 0.038). In contrast, the relative gene expression of the extracellular fatty acid-binding protein (EXFAB) and peroxisome proliferator-activated receptor-γ (PPAR-γ) were positively correlated (r = 0.48, p = 0.042 and r = 0.50, p = 0.031) with the overall ADFI of birds. Conclusion: Taken together, the results of this study suggest that the combination of in ovo feeding of GABA and ETM can enhance hepatic antioxidant function in broilers.

Abstract

Objective: This study investigated the effects of in ovo feeding of γ-aminobutyric acid (GABA) and embryonic thermal manipulation (ETM) on growth performance, organ indices, plasma biochemical parameters, hepatic antioxidant levels, and expression of lipid metabolism-related genes in broilers. Methods: Two hundred and fifty eggs were assigned to one of four treatments: control eggs incubated under standard conditions (CON); eggs that received an in ovo injection of 10% GABA on day 17.5 of incubation (G10); thermally manipulated eggs between days 10 and 18 of incubation at 39.6°C for 6 h daily (TM); and eggs that received both treatments during incubation (G10+TM). After 28 days of rearing, five birds per treatment were selected for blood and organ sampling. Results: No differences were found in hatchability or growth parameters among different treatment groups. Hepatic gene expression of catalase (CAT) and glutathione peroxidase 1 (GPx1) was upregulated (p = 0.046 and p = 0.006, respectively) in the G10+TM group, while that of nuclear factor erythroid 2–related factor 2 (NRF2) was upregulated (p = 0.039) in the G10 group. In addition, the relative gene expression of NADPH oxidase 1 (NOX1) was significantly lower (p = 0.007) in all treatment groups than that in the CON group. Hepatic fatty acid synthase (FAS) levels and average daily feed intake (ADFI) of last week showed a positive correlation (r = 0.50, p = 0.038). In contrast, the relative gene expression of the extracellular fatty acid-binding protein (EXFAB) and peroxisome proliferator-activated receptor-γ (PPAR-γ) were positively correlated (r = 0.48, p = 0.042 and r = 0.50, p = 0.031) with the overall ADFI of birds. Conclusion: Taken together, the results of this study suggest that the combination of in ovo feeding of GABA and ETM can enhance hepatic antioxidant function in broilers.

참고문헌 (38)

  1. Surai PF, "Antioxidant systems in poultry biology : superoxide dismutase", J Anim Res Nutr, 1, pp.8~ (2016)
  2. Goel A, "Regulation of gene expression in chickens by heat stress", J Anim Sci Biotechnol, 12, pp.11~ (2021)[DOI]
  3. Surai PF, "Redox homeostasis in poultry : regulatory roles of NF-κB", Antioxidants, 10, pp.186~ (2021)[DOI]
  4. Chueh CC, "Antioxidant capacity of banana peel and its modulation of Nrf2-ARE associated gene expression in broiler chickens", Ital J Anim Sci, 18, pp.1394~1403 (2019)
  5. Chelikani P, "Diversity of structures and properties among catalases", Cell Mol Life Sci, 61, pp.192~208 (2004)[DOI]
  6. Ma Q, "Role of nrf2 in oxidative stress and toxicity", Annu Rev Pharmacol Toxicol, 53, pp.401~426 (2013)[DOI]
  7. Ncho CM, "The effect of gammaaminobutyric acid supplementation on growth performances, immune responses, and blood parameters of chickens reared under stressful environment : a meta-analysis", Environ Sci Pollut Res, 28, pp.45019~45028 (2021)[DOI]
  8. Cobb SR, "Synchronization of neuronal activity in hippocampus by individual GABAergic interneurons", Nature, 378, pp.75~78 (1995)[DOI]
  9. El-Naggar K, "Role of gamma-aminobutyric acid in regulating feed intake in commercial broilers reared under normal and heat stress conditions", J Therm Biol, 84, pp.164~175 (2019)[DOI]
  10. Hu H, "Interactive effects of glutamine and gamma-aminobutyric acid on growth performance and skeletal muscle amino acid metabolism of 22-42-dayold broilers exposed to hot environment", Int J Biometeorol, 60, pp.907~915 (2016)[DOI]
  11. 최양호, "여름철 γ-aminobutyric acid 급여가 산란계 폐사율에 미치는 영향", 농업생명과학연구, 53(2), pp.131~139 (2019), 농업생명과학연구원[DOI]
  12. Ncho CM, "In ovo injection of gaba can help body weight gain at hatch, increase chick weight to egg weight ratio, and improve broiler heat resistance", Animals, 11, pp.1364~ (2021)[DOI]
  13. Ncho CM, "Effect of thermal conditioning on growth performance and thermotolerance in broilers : A systematic review and meta-analysis", J Therm Biol, 98, pp.102916~ (2021)[DOI]
  14. Al-Zghoul MB, "Effects of thermal manipulation of eggs on the response of jejunal mucosae to posthatch chronic heat stress in broiler chickens", Poult Sci, 99, pp.2727~2735 (2020)[DOI]
  15. Ncho CM, "Effects of in ovo feeding of γ-aminobutyric acid on growth performances, plasma metabolites, and antioxidant status in broilers exposed to cyclic heat stress", Sustainability, 13, pp.11032~ (2021)[DOI]
  16. Goel A, "Embryonic thermal manipulation and in ovo gamma-aminobutyric acid supplementation regulating the chick weight and stress-related genes at hatch", Front Vet Sci, 8, pp.807450~ (2022)[DOI]
  17. Zhang H, "Effects of in ovo injection of L-ascorbic acid on growth performance, carcass composition, plasma antioxidant capacity, and meat quality in broiler chickens", Poult Sci, 98, pp.3617~3625 (2019)[DOI]
  18. Slawinska A, "TLR-mediated cytokine gene expression in chicken peripheral blood mononuclear cells as a measure to characterize immunobiotics", Genes, 12, pp.195~ (2021)[DOI]
  19. Soleimani AF, "Physiological responses of 3 chicken breeds to acute heat stress", Poult Sci, 90, pp.1435~1440 (2011)[DOI]
  20. Surai PF, "Antioxidant defence systems and oxidative stress in poultry biology : an update", Antioxidants, 8, pp.235~ (2019)[DOI]
  21. Kirkman HN, "Catalase : a tetrameric enzyme with four tightly bound molecules of NADPH", Proc Natl Acad Sci, 81, pp.4343~4347 (1984)[DOI]
  22. Surai P, "Antioxidant systems in poultry biology : nutritional modulation of vitagenes", Eur Poult Sci, 81, pp.214~ (2017)[DOI]
  23. Al Wakeel RA, "Alleviation by gamma amino butyric acid supplementation of chronic heat stress-induced degenerative changes in jejunum in commercial broiler chickens", Stress, 20, pp.562~572 (2017)[DOI]
  24. Chen Z, "Protective effect of γ-aminobutyric acid on antioxidation function in intestinal mucosa of Wenchang chicken induced by heat stress", J Anim Plant Sci, 23, pp.1634~1641 (2013)
  25. Walls AB, "The glutamine-glutamate", GABA cycle : function, regional differences in glutamate and GABA production and effects of interference with GABA metabolism, 2015, pp.40, 402~409
  26. Al-Zghoul MB, "Effect of thermal manipulation of broilers embryos on the response to heatinduced oxidative stress", Poult Sci, 98, pp.991~1001 (2019)[DOI]
  27. Saleh KM, "Embryonic thermal manipulation affects the antioxidant response to post-hatch thermal exposure in broiler chickens", Animals, 10, pp.126~ (2020)[DOI]
  28. Zaefarian F, "Avian liver : the forgotten organ", Animals, 9, pp.63~ (2019)[DOI]
  29. Hicks JA, "Discovery of chicken microRNAs associated with lipogenesis and cell proliferation", Physiol Genomics, 41, pp.185~193 (2010)[DOI]
  30. Wang G, "Factors affecting adipose tissue development in chickens : a review", Poult Sci, 96, pp.3687~3699 (2017)[DOI]
  31. Ohta Y, "Optimum site for in ovo amino acid injection in broiler breeder eggs", Poult Sci, 80, pp.1425~1429 (2001)[DOI]
  32. Narinç D, "Effects of thermal manipulations during embryogenesis of broiler chickens on developmental stability, hatchability and chick quality", Animal, 10, pp.1328~1335 (2016)[DOI]
  33. Piestun Y, "Thermal manipulations of broiler embryos-The effect on thermoregulation and development during embryogenesis", Poult Sci, 88, pp.2677~2688 (2009)[DOI]
  34. Kadam MM, "Effect of in ovo threonine supplementation on early growth, immunological responses and digestive enzyme activities in broiler chickens", Br Poult Sci, 49, pp.736~741 (2008)[DOI]
  35. Piestun Y, "Thermal manipulations during broiler embryogenesis improves post-hatch performance under hot conditions", J Therm Biol, 36, pp.469~474 (2011)[DOI]
  36. Riras A, "Indication of immunodepression in chicken infected with various strain of Marck's dsease virus", Avian Dis, 32, pp.1~8 (1988)
  37. Tang J, "The protective effect of gamma-aminobutyric acid on the development of immune function in chickens under heat stress", J Anim Physiol Anim Nutr(Berl), 100, pp.768~777 (2016)[DOI]
  38. Jin Z, "GABA is an effective immunomodulatory molecule", Amino Acids, 45, pp.87~94 (2013)[DOI]

관련 논문AI추천

Effects of gamma aminobutyric acid on performance, blood cell of broiler subjected to multi-stress environmentsHan Jong-Kwon, Joo Younghye, Oh Mihyang, Park Keun-tae아세아·태평양축산학회 · Animal Bioscience2023 · 제36권 제2호Pre-hatch thermal manipulation and post-hatch guanidinoacetic acid supplementation enhance meat quality under chronic heat stress in broiler chickensGholamreza Zaboli한국식품저장유통학회 · Food Science and Preservation2025 · 제32권 제6호L-Leucine increases the daily body temperature and affords thermotolerance in broiler chicksGuofeng Han, Hui Yang, Kosuke Tashiro, Mitsuhiro Furuse, Shogo Haraguchi, Takashi Bungo, Takuro Miyazaki, Vishwajit S. Chowdhury, Yunhao Wang아세아·태평양축산학회 · Animal Bioscience2019 · 제32권 제6호Rhythmic light exposure during incubation enhances liver development and lipid accumulation in chicken embryosCui Yanjun, Li Fang, Liu Lu, Li Xiangchen, Song Dan, Wang Panlin, Wu Yongshu, Xie Xiuyu, Zhang Jiayi아세아·태평양축산학회 · Animal Bioscience2026 · 제39권 제1호Effects of maternal dietary energy restriction on laying performance, embryonic development, and lipid metabolism in broilersChen Zhihui, Lian Lina, Ma Chenzhan, Niu Shupeng, Sun Hao, Sun Jinhua, Xu Liangmei, Zhao Zeyu아세아·태평양축산학회 · Animal Bioscience2022 · 제35권 제5호여름철 γ-aminobutyric acid 급여가 산란계 폐사율에 미치는 영향최양호농업생명과학연구원 · 농업생명과학연구2019 · 제53권 제2호

이 간행물의 다른 논문 (21건)