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운동 강도 차이에 따른 흰쥐의 비복근에서 근원성 조절인자와 Extracellular Signal-Regulated Kinases의 발현에 관한 연구

Effects of either low-or moderate-to-high- intensity treadmill running on the activation of ERK1/2, JNK1/2 and MyoD, Myogenin was determined in rat gastrocnemius

박한수|채정룡|김형준
한국운동생리학회|2008.05|운동과학 (Exercise Science)|제17권 제2호|pp. 129-142|DOI 10.15857/ksep.2008.17.2.129

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키워드

ERK1/2pERK1/2JNK1/2 pJNK1/2Myogenin mRNAMyoD mRNA얼크1/2피얼크1/2정크1/2피정크1/2미오겐 mRNA미오디 mRNA

초록

Kim, H.J., Park, H.S., Chae, J.R. Effects of either low-or moderate-to-high- intensity treadmill running on the activation of ERK1/2, JNK1/2 and MyoD, Myogenin was determined in rat gastrocnemius. Exercise Science. 17(2): 129-142, 2008. Sprague-Dawley rats were assigned to one of three groups: (i) sedentary(NT; n=10); (ii) low-intensity exercise(8m/min; LT; n=10); and (iii) moderate- to-high-intensity exercise(28m /min; HT; n=10). The training regimens were planned so that animals covered the same distance and had similar glycogen utilization for both LT and HT exercise sessions. Western blot analyzed the Revelation of Myogenic regulatory factors and Extracellular signal-regulated kinases in the gastrocnemius muscle of rats; First, After four Weeks Exercise, after the last exercise bout obtained samples. there were no differences in the final BW of animals from any of the three groups. and MyoD mRNA increased LT (1.55times) and HT(1.75times), Myogenin mRNA increased LIT(1.05times) and HIT(2.01times), pERK1 increased LT(1.22times) and HT(4.4times), pERK2 increased LT(1.68times) and HT (2.39times), pJNK1 increased LT(1.98times) and HT(2.68times), the pJNK2 increased LT(2.05times) and HT(2.24times) comparing with the NT group(p<.05). Second, After four weeks exercise, 24h after the last exercise bout obtained samples. MyoD mRNA increased LT(1.71times) and HT(1.63times), The Myogenin mRNA increased LT(1.64times) and HT(1.05times), pERK1 increased LT(1.16times) and HT(2.1times), The pERK2 increased LT(1.09times) and HT (2.17times), The pJNK1 increased LT(1.11 times) and HT(1.03times), The pJNK2 increased LT(1.37times) and HT(1.49times) comparing with the NT group(p<.05). All data were normalized against the corresponding sedentary value compared with exercise. Four weeks exercise of different intensities results in gastrocnemius muscle activation of Extracellular signal pathways and MRF mRNA expression, which may be one mechanism regulating specific adaptations induced by different exercise intensities.

Abstract

Kim, H.J., Park, H.S., Chae, J.R. Effects of either low-or moderate-to-high- intensity treadmill running on the activation of ERK1/2, JNK1/2 and MyoD, Myogenin was determined in rat gastrocnemius. Exercise Science. 17(2): 129-142, 2008. Sprague-Dawley rats were assigned to one of three groups: (i) sedentary(NT; n=10); (ii) low-intensity exercise(8m/min; LT; n=10); and (iii) moderate- to-high-intensity exercise(28m /min; HT; n=10). The training regimens were planned so that animals covered the same distance and had similar glycogen utilization for both LT and HT exercise sessions. Western blot analyzed the Revelation of Myogenic regulatory factors and Extracellular signal-regulated kinases in the gastrocnemius muscle of rats; First, After four Weeks Exercise, after the last exercise bout obtained samples. there were no differences in the final BW of animals from any of the three groups. and MyoD mRNA increased LT (1.55times) and HT(1.75times), Myogenin mRNA increased LIT(1.05times) and HIT(2.01times), pERK1 increased LT(1.22times) and HT(4.4times), pERK2 increased LT(1.68times) and HT (2.39times), pJNK1 increased LT(1.98times) and HT(2.68times), the pJNK2 increased LT(2.05times) and HT(2.24times) comparing with the NT group(p<.05). Second, After four weeks exercise, 24h after the last exercise bout obtained samples. MyoD mRNA increased LT(1.71times) and HT(1.63times), The Myogenin mRNA increased LT(1.64times) and HT(1.05times), pERK1 increased LT(1.16times) and HT(2.1times), The pERK2 increased LT(1.09times) and HT (2.17times), The pJNK1 increased LT(1.11 times) and HT(1.03times), The pJNK2 increased LT(1.37times) and HT(1.49times) comparing with the NT group(p<.05). All data were normalized against the corresponding sedentary value compared with exercise. Four weeks exercise of different intensities results in gastrocnemius muscle activation of Extracellular signal pathways and MRF mRNA expression, which may be one mechanism regulating specific adaptations induced by different exercise intensities.

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