8주간의 사다리 등반 운동이 젊은 쥐와 중년 쥐의 골격근 내 PGC-1α 발현과 미토콘드리아 생합성에 미치는 영향
The Effects of Latter Climbing Exercise Training on PGC-1α Expression and Mitochondrial Biogenesis of Skeletal Muscle in Young and Middle-Aged Rats
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본 연구의 목적은 젊은 쥐와 중년 쥐를 대상으로 사다리 등반 운동이 골격근 내 PGC-1α의 발현과 미토콘드리아 생합성에 미치는 영향을 규명하는 것이다. 50주령과 10주령의 수컷 Wistar 흰쥐 40마리를 대상으로 1주간의 환경 적응기간을 거친 후 좌업집단과 운동집단으로 무선 배정하였다. 사다리 등반 운동은 주당 3회 총 8주간 처치하였다. 연구결과 8주간의 사다리 등반운동은 골격근 내 AMPK를 활성화시켜 PGC-1α의 발현을 증가시킴으로써 미토콘드리아 생합성을 증가시킨 것으로 나타났다. 그러나 노화에 따라 미토콘드리아 생합성의 정도는 유의하게 감소하였으며, 이는 AMPK의 활성도와 PGC-1α의 발현정도의 변화 없이 나타났다. 이상의 결과에서 사다리 등반 운동은 젊은 쥐와 중년 쥐 모두의 골격근 내 미토콘드리아 생합성의 증가시키는 것으로 나타났으나, 동일한 운동을 실시하여도 운동효과는 연령의 증가에 따라서 감소하는 것으로 나타났다.
Abstract
The purpose of this study was to investigate effects of ladder climbing exercise training on PGC-1α expression and mitochondrial biogenesis of skeletal muscle in young and middle-aged rats. 50 weeks and 10 weeks of male Wistar rats were randomly assigned for exercise and sedentary groups after one week of adaptation period. The ladder climbing exercise training was carried out using ladder climbing with weight attached to the tail (3 days/week, 8 weeks). Ladder climbing exercise training for 8 weeks significantly increased AMPK activity and PGC-1α protein, mitochondrial enzyme activity in plantaris muscle. However mitochondrial biogenesis levels were significantly decreased in 50 weeks old rats without the change of PGC-1 level and AMPK activity. Therefore ladder climbing exercise training was effective tool to increase mitochondrial biogenesis in skeletal muscle, but training responses were found in the middle-aged rats.
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