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근수축에 의한 Nitric Oxide 생성과 미토콘드리아 생합성 조절체계에서 CaMKII의 작용

The Effect of CaMKII Mediated Nitric Oxide formation on Skeletal Muscle Contraction Induced Mitochondria Biogenesis

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초록

PURPOSE: This study was to investigate the effect of CaMKII mediated nitric oxide formation on skeletal muscle contraction induced mitochondria biogenesis. METHODS: To examine the effect of CaMKII inhibition on nitric oxide synthase regulation during muscle contraction or exercise, we designed in vivo and in vitro study. In vitro study was skeletal muscle primary study for identify relation between CaMKII and NOS and in vivo study was treadmill running study for NO formation regulation by nNOS or eNOS in tissue level. RESULTS: In skeletal muscle primary cell, nNOS inhibition by nNOS inhibitor or nNOS KD inhibited contraction induced NO formation or mitochondria biogenesis related factor upregulation including PGC-1α deacetylation or mitochondrial mRNA but not by eNOS inhibitor or eNOS KD. CaMKII inhibition by AIP attenuated contraction induced NO formation, nNOS phosphorylation and mitochondria biogenesis related factor upregulation including PGC-1α deacetylation or mitochondrial mRNA. However in vivo nNOS or eNOS KD both inhibited NO formation and mitochondria biogenesis related factor upregulation including PGC-1α deacetylation or mitochondrial mRNA during treadmill running. CONCLUSIONS: These results suggest that increase of CaMKII activity regulates NO formation during muscle contraction via regulation of nNOS phosphorylation and plays pivotal role in mitochondria biogenesis.

Abstract

PURPOSE: This study was to investigate the effect of CaMKII mediated nitric oxide formation on skeletal muscle contraction induced mitochondria biogenesis. METHODS: To examine the effect of CaMKII inhibition on nitric oxide synthase regulation during muscle contraction or exercise, we designed in vivo and in vitro study. In vitro study was skeletal muscle primary study for identify relation between CaMKII and NOS and in vivo study was treadmill running study for NO formation regulation by nNOS or eNOS in tissue level. RESULTS: In skeletal muscle primary cell, nNOS inhibition by nNOS inhibitor or nNOS KD inhibited contraction induced NO formation or mitochondria biogenesis related factor upregulation including PGC-1α deacetylation or mitochondrial mRNA but not by eNOS inhibitor or eNOS KD. CaMKII inhibition by AIP attenuated contraction induced NO formation, nNOS phosphorylation and mitochondria biogenesis related factor upregulation including PGC-1α deacetylation or mitochondrial mRNA. However in vivo nNOS or eNOS KD both inhibited NO formation and mitochondria biogenesis related factor upregulation including PGC-1α deacetylation or mitochondrial mRNA during treadmill running. CONCLUSIONS: These results suggest that increase of CaMKII activity regulates NO formation during muscle contraction via regulation of nNOS phosphorylation and plays pivotal role in mitochondria biogenesis.

참고문헌 (42)

  1. Holloszy JO, "Adaptations of skeletal muscle to endurance exercise and their metabolic consequences", Journal of Applied Physiology, 56(4), pp.831~838 (1984)
  2. Russell AP, "Skeletal muscle mitochondria:a major player in exercise, health and disease", Biochimica et Biophysica Acta (BBA)-General Subjects, 1840(4), pp.1276~1284 (2013)
  3. Yoshida Y, "Exercise-and training-induced upregulation of skeletal muscle fatty acid oxidation are not solely dependent on mitochondrial machinery and biogenesis", The Journal of Physiology, 15(591(Pt 18)), pp.4415~4426 (2013)
  4. Tengan CH, "Nitric oxide in skeletal muscle: role on mitochondrial biogenesis and function", International Journal of Molecular Sciences, 13(12), pp.17160~17184 (2013)
  5. Lira VA, "Nitric oxide increases GLUT4 expression and regulates AMPK signaling in skeletal muscle", American Journal of Physiology-Endocrinology and Metabolism, 293(4), pp.E1062~E1068 (2007)
  6. Nisoli E, "Mitochondrial biogenesis by NO yields functionally active mitochondria in mammals", Proceedings of the National Academy of Sciences of the United States of America, 101(47), pp.16507~16512 (2004)
  7. Lira VA, "Nitric oxide and AMPK cooperatively regulate PGC-1 in skeletal muscle cells", The Journal of Physiology, 15(588(Pt 18)), pp.3551~3566 (2010)
  8. Toledo JC, "Connecting the chemical and biological properties of nitric oxide", Chemical Research in Toxicology, 25(5), pp.975~989 (2012)
  9. Eghbalzadeh K, "Skeletal muscle nitric oxide (NO) synthases and NO-signaling in “diabesity”--what about the relevance of exercise training interventions?", Nitric Oxide, 37, pp.28~40 (2014)
  10. Jin BY, "Endothelial nitric oxide synthase negatively regulates hydrogen peroxide-stimulated AMP-activated protein kinase in endothelial cells", Proceedings of the National Academy of Sciences, 106(41), pp.17343~17348 (2009)
  11. Dudzinski DM1, "The regulation and pharmacology of endothelial nitric oxide synthase", Annual Review Pharmacology Toxicology, 46, pp.235~276 (2006)
  12. Trevellin E, "Exercise training induces mitochondrial biogenesis and glucose uptake in subcutaneous adipose tissue through eNOS-dependent mechanisms", Diabetes, 63(8), pp.2800~2811 (2014)
  13. Tengan CH, "The role of nitric oxide in muscle fibers with oxidative phosphorylation defects", Biochemical and Biophysical Research Communications, 359(3), pp.771~777 (2007)
  14. Nisoli E, "Mitochondrial biogenesis in mammals : the role of endogenous nitric oxide", Science, 299(5608), pp.896~899 (2003)
  15. Ojuka EO, "Regulation of GLUT4 biogenesis in muscle: evidence for involvement of AMPK and Ca(2+)", American Journal of Physiology-Endocrinology and Metabolism, 282(5), pp.1008~1013 (2002)
  16. Freyssenet D, "Calcium-dependent regulation of cytochrome c gene expression in skeletal muscle cells. Identification of a protein kinase c-dependent pathway", Journal of Biological Chemistry, 274(14), pp.9305~9311 (1999)
  17. Chin ER, "A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type", Genes & Development, 12(16), pp.2499~2509 (1998)
  18. Wright DC, "Calcium induces increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha and mitochondrial biogenesis by a pathway leading to p38 mitogen-activated protein kinase activation", Journal of Biological Chemistry, 282(26), pp.18793~18799 (2007)
  19. Uldry M, "Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation", Cell Metabolism, 3(5), pp.333~341 (2006)
  20. Curran J, "Nitric oxide-dependent activation of CaMKII increases diastolic sarcoplasmic reticulum calcium release in cardiac myocytes in response to adrenergic stimulation", PLoS One, 9(2), pp.e87495~ (2014)
  21. Park DR, "Exercise ameliorates insulin resistance via Ca2+ signals distinct from those of insulin for GLUT4 translocation in skeletal muscles", Diabetes, 64(4), pp.1224~1234 (2015)
  22. Liu Y, "Activity-dependent nuclear translocation and intranuclear distribution of NFATc in adult skeletal muscle fibers", The Journal of Cell Biology, 155(1), pp.27~39 (2001)
  23. Schefer V, "Oxygen consumption in adult and AGED C57BL", 6J mice during acute treadmill exercise of different intensity, 1996, pp.31(3), 387~392
  24. Merry TL, "Mitohormesis in exercise training", Free Radical Biology and Medicine, S0891-5849(15), pp.01141~01147 (2015)
  25. 김상현, "단기간과 장기간 지구성운동 시 쥐의 골격근 내 항산화 효소와 미토콘드리아 효소의 발현양상", 운동과학, 20(4), pp.359~366 (2011), 한국운동생리학회[DOI]
  26. Forstermann U, "Nitric oxide synthases: regulation and function", European Heart Journal, 33(7), pp.829~837 (2012)
  27. Torres SH, "Inflammation and nitric oxide production in skeletal muscle of type 2diabetic patients", Journal of Endocrinology, 181(3), pp.419~427 (2004)
  28. Brenman JE, "Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy", Cell, 82(5), pp.743~752 (1995)
  29. Thomas GD, "Vasomodulation by skeletal muscle-derived nitric oxide requires alphasyntrophin-mediated sarcolemmal localization of neuronal Nitric oxide synthase", Circulation research, 92(5), pp.554~560 (2003)
  30. Tengan CH, "Nitric oxide in skeletal muscle: role on mitochondrial biogenesis and function", International journal of molecular sciences, 13(12), pp.17160~17184 (2012)
  31. Bates TE, "Immunocytochemical evidence for a mitochondrially located nitric oxide synthase in brain and liver", Biochemical and Biophysical Research Communications, 213(3), pp.896~900 (1995)
  32. Nosarev AV, "Exercise and NO production: relevance and implications in the cardiopulmonary system", Frontiers in Cell and Developmental Biology, 7(2), pp.73~ (2015)
  33. Shabeeh H, "Sympathetic activation increases NO release from eNOS but neither eNOS nor nNOS play an essential role in exercise hyperemia in the human forearm", American Journal of Physiology-Heart and Circulatory Physiology, 304(9), pp.H1225~H1230 (2013)
  34. Louie JC, "The interactive contributions of Na+", K+‐ATPase and nitric oxide synthase to sweating and cutaneous vasodilation during exercise in the heat, 2016, ~
  35. Wadley GD, "Effect of nitric oxide synthase inhibition on mitochondrial biogenesis in rat skeletal muscle", Journal of Applied Physiology, 102(1), pp.314~320 (2007)
  36. Nisoli E, "Mitochondrial biogenesis by NO yields functionally active mitochondria in mammals", Proceedings of The National Academy of Sciences of the United States of America, 101(47), pp.16507~16512 (2004)
  37. Hudmon A, "Structure-function of the multifunctional Ca2+", calmodulin-dependent protein kinase II, 2002, pp.364(3), 593~611
  38. Jain SS, "High-Fat Diet-Induced Mitochondrial Biogenesis Is Regulated by Mitochondrial-Derived Reactive Oxygen Species Activation of CaMKII", Diabetes, 63(6), pp.1907~1913 (2014)
  39. Wang M, "Ischemic preconditioning protects the brain against injury via inhibiting CaMKII-nNOS signaling pathway", Brain Research, 1634, pp.140~149 (2016)
  40. Song T, "Nitric oxide prevents phosphorylation of neuronal nitric oxide synthase at serine1412 by inhibiting the Akt", PKB and CaM-K II signaling pathways, 2012, pp.30(1), 15~20
  41. Garcia-Roves PM, "Role of calcineurin in exerciseinduced mitochondrial biogenesis", American Journal of Physiology-Endocrinology and Metabolism, 290(6), pp.E1172~E1179 (2006)
  42. Rao X, "Exercise protects against diet-induced insulin resistance through downregulation of protein kinase Cβ in mice", PLoS One, 8(12), pp.e81364~ (2013)

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