본문 바로가기
KERD
논문KCI 등재

장기간의 운동이 대장암 유발 쥐의 SPARC와 OSM발현에 미치는 영향

The Effects of Long-term Exercise on the Expression of SPARC and OSM in Colon Cancer-Induced Mice

조은효|전태원|김준홍
한국운동생리학회|2016.05|운동과학 (Exercise Science)|제25권 제2호|pp. 76-84|DOI 10.15857/ksep.2016.25.2.76

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

마이오카인MyokineColon cancerTreadmill exercise대장암SPARCOSM트레드밀운동

초록

PURPOSE: The purpose of this study was to investigate the impacts of long-term treadmill exercise on the expression of SPARC and OSM in muscle, blood and colon polyp of colon cancer-induced mice. METHODS: Thirty mice were randomly divided into two groups: Exercise group (EX, n=15), Control group (CON, n=15). Mice of EX, at 6-week of old, performed exercise at moderate intensity for 30 minutes, 5 days per week, during 12 weeks. At 17-week of old, all mice were injected AOM and DSS to induce colon cancer. Then EX kept doing exercise for another 12 weeks. The expression of OSM and SPARC in muscle, blood, colon polyp was analyzed using the ELISA kit. RESULTS: The OSM expression of EX was significantly higher than CON in muscle (p<.01), but not in blood and polyps. On the other hand, the SPARC expression of EX was significantly increased in colon polyp (p<.01) compared with CON, however, there is no significant difference in blood and muscle. CONCLUSIONS: A 24-week treadmill exercise induced over-expression of SPARC and OSM in different tissues of

Abstract

PURPOSE: The purpose of this study was to investigate the impacts of long-term treadmill exercise on the expression of SPARC and OSM in muscle, blood and colon polyp of colon cancer-induced mice. METHODS: Thirty mice were randomly divided into two groups: Exercise group (EX, n=15), Control group (CON, n=15). Mice of EX, at 6-week of old, performed exercise at moderate intensity for 30 minutes, 5 days per week, during 12 weeks. At 17-week of old, all mice were injected AOM and DSS to induce colon cancer. Then EX kept doing exercise for another 12 weeks. The expression of OSM and SPARC in muscle, blood, colon polyp was analyzed using the ELISA kit. RESULTS: The OSM expression of EX was significantly higher than CON in muscle (p<.01), but not in blood and polyps. On the other hand, the SPARC expression of EX was significantly increased in colon polyp (p<.01) compared with CON, however, there is no significant difference in blood and muscle. CONCLUSIONS: A 24-week treadmill exercise induced over-expression of SPARC and OSM in different tissues of

참고문헌 (55)

  1. Pedersen BK, "Muscle as an endocrine organ: focus on muscle-derived interleukin-6", Physiological reviews, 88(4), pp.1379~1406 (2008)
  2. Pedersen BK, "Physiological roles of muscle-derived interleukin-6 in response to exercise", Current Opinion in Clinical Nutrition and Metabolic Care, 10(3), pp.265~271 (2007)
  3. Rohde T, "Prolonged submaximal eccentric exercise is associated with increased levels of plasma IL-6", The American Journal of Physiology, 273(1 Pt 1), pp.E85~E91 (1997)
  4. Broholm C, "Leukaemia inhibitory factor--an exercise-induced myokine", Exercise immunology review, 16, pp.77~85 (2010)
  5. Hojman P, "Exercise-induced muscle-derived cytokines inhibit mammary cancer cell growth. American journal of physiology", Endocrinology and Metabolism, 301(3), pp.E504~E510 (2011)
  6. Bornstein P, "Matricellular proteins: extracellular modulators of cell function", Current Opinion in Cell Biology, 14(5), pp.608~616 (2002)
  7. Hasselaar P, "SPARC antagonizes the effect of basic fibroblast growth factor on the migration of bovine aortic endothelial cells", Journal of Cellular Biochemistry, 49(3), pp.272~283 (1992)
  8. Yan Q, "SPARC, a matricellular glycoprotein with important biological functions", Journal of Histochemistry and Cytochemistry, 47(12), pp.1495~1506 (1999)
  9. Tai IT, "SPARC in cancer biology: its role in cancer progression and potential for therapy", Drug Resistance Updates, 11(6), pp.231~246 (2008)
  10. Bellahcene A, "Increased expression of osteonectin and osteopontin, two bone matrix proteins, in human breast cancer", The American Journal of Pathology, 146(1), pp.95~100 (1995)
  11. Yang E, "Frequent inactivation of SPARC by promoter hypermethylation in colon cancers", International Journal of Cancer, 121(3), pp.567~575 (2007)
  12. 박상일, "상피성 난소종양에서 SPARC 발현과 임상병리학적 연관성에 관한 연구", Obstetrics & Gynecology Science, 54(12), pp.770~777 (2011), 대한산부인과학회
  13. Aoi W, "A novel myokine, secreted protein acidic and rich in cysteine (SPARC), suppresses colon tumorigenesis via regular exercise", Gut, 62(6), pp.882~889 (2012)
  14. Tai IT, "Genome-wide expression analysis of therapy-resistant tumors reveals SPARC as a novel target for cancer therapy", The Journal of Clinical Investigation, 115(6), pp.1492~1502 (2005)
  15. Grant SL, "The oncostatin M signalling pathway: reversing the neoplastic phenotype?", Molecular Medicine Today, 5(9), pp.406~412 (1999)
  16. Bolin C, "Oncostatin m promotes mammary tumor metastasis to bone and osteolytic bone degradation", Genes & Cancer, 3(2), pp.117~130 (2012)
  17. 김창선, "Expression of bone-resorbing cytokines according to aerobic exercise intensities", Journal of Exercise Nutrition & Biochemistry, 15(3), pp.131~139 (2011), 한국운동영양학회
  18. 김철우, "16주 저항성 트레이닝이 엘리트 역도선수의 피로물질과 근 손상, 산화적 손상, myokine에 미치는 영향", 생명과학회지, 22(2), pp.184~191 (2012), 한국생명과학회
  19. Febbraio MA, "Muscle-derived interleukin-6: mechanisms for activation and possible biological roles", FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 16(11), pp.1335~1347 (2002)
  20. Tamura Y, "Upregulation of circulating IL-15 by treadmill running in healthy individuals:is IL-15 an endocrine mediator of the beneficial effects of endurance exercise?", Endocrine journal, 58(3), pp.211~215 (2011)
  21. Neufert C, "An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression", Nature Protocols, 2(8), pp.1998~2004 (2007)
  22. Bedford TG, "Maximum oxygen consumption of rats and its changes with various experimental procedures", Journal of Applied Physiology, 47(6), pp.1278~1283 (1979)
  23. Solymoss BC, "Incidence and clinical characteristics of the metabolic syndrome in patients with coronary artery disease", Coronary Artery Disease, 14(3), pp.207~212 (2003)
  24. Wheelwright S, "A systematic review of health-related quality of life instruments in patients with cancer cachexia", Supportive Care in Cancer, 21(9), pp.2625~2636 (2013)
  25. Bachmann J, "Cachexia worsens prognosis in patients with resectable pancreatic cancer", Journal of Gastrointestinal Surgery, 12(7), pp.1193~1201 (2008)
  26. Meriggi F, "Cancer Cachexia: One Step Ahead", Reviews on recent clinical trials, 10(3), pp.246~250 (2015)
  27. Giordano A, "Skeletal muscle metabolism in physiology and in cancer disease", Journal of Cellular Biochemistry, 90(1), pp.170~186 (2003)
  28. Woods JA, "Exercise and resistance to neoplasia", Canadian Journal of Physiology and Pharmacology, 76(5), pp.581~588 (1998)
  29. 임인수, "노화와 운동 효과에 대한 항산화, 내분비, 면역학적 접근", 운동과학, 11(1), pp.35~51 (2002), 한국운동생리학회
  30. Ardies CM, "Exercise, cachexia, and cancer therapy: a molecular rationale", Nutrition and Cancer, 42(2), pp.143~157 (2002)
  31. Baltgalvis KA, "The interaction of a high-fat diet and regular moderate intensity exercise on intestinal polyp development in Apc Min", + mice, 2009, pp.2(7), 641~649
  32. Zhou X, "Reversal of cancer cachexia and muscle wasting by ActRIIB antagonism leads to prolonged survival", Cell, 142(4), pp.531~543 (2010)
  33. Daneryd PL, "Effects of spontaneous physical exercise on experimental cancer anorexia and cachexia", European Journal of Cancer and Clinical Oncology, 26(10), pp.1083~1088 (1990)
  34. Baltgalvis KA, "Effect of exercise on biological pathways in ApcMin", + mouse intestinal polyps, 2008, pp.104(4), 1137~1143
  35. Colbert LH, "Exercise and intestinal polyp development in APCMin mice", Medicine and Science in Sports and Exercise, 35(10), pp.1662~1669 (2003)
  36. Baltgalvis KA, "Activity level, apoptosis, and development of cachexia in Apc(Min", +)mice, 2010, pp.109(4), 1155~1161
  37. Lee IM, "Quetelet’s index and risk of colon cancer in college alumni", Journal of the National Cancer Institute, 84(17), pp.1326~1331 (1992)
  38. Murphy TK, "Body mass index and colon cancer mortality in a large prospective study", American Journal of Epidemiology, 152(9), pp.847~854 (2000)
  39. 김문찬, "신체활동이 한국성인의 대장용종 발생에 미치는 영향", Korean Journal of Family Medicine, 26(7), pp.17~22 (2005), 대한가정의학회
  40. Park HS, "Relationship of obesity and visceral adiposity with serum concentrations of CRP, TNF-alpha and IL-6", Diabetes research and clinical practice, 69(1), pp.29~35 (2005)
  41. 이항락, "대장 선종의 위험 인자로 복부 비만 및 인슐린 저항성에 대한 연구", 대한소화기학회지, 49(3), pp.147~151 (2007), 대한소화기학회
  42. Kondo T, "Effect of exercise on circulating adipokine levels in obese young women", Endocrine Journal, 53(2), pp.189~195 (2006)
  43. 김기진, "중년여성의 신체구성과 운동참여여부에 따른 혈중 IL-6 및 TNF-α 농도의 관련성", 운동과학, 17(2), pp.119~128 (2008), 한국운동생리학회
  44. Winters-Stone KM, "Resistance Exercise Reduces Body Fat and Insulin During Androgen-Deprivation Therapy for Prostate Cancer", Oncology Nursing Forum, 42(4), pp.348~356 (2015)
  45. Ju J, "Voluntary exercise inhibits intestinal tumorigenesis in Apc(Min", +) mice and azoxymethane, BMC cancer, pp.2008, 8[DOI]
  46. Kos K, "SPARC: a key player in the pathologies associated with obesity and diabetes. Nature reviews", Endocrinology, 6(4), pp.225~235 (2010)
  47. Yang EG, "Identification and clinical signification of SPARC inactivation through promoter hypermethylation in human colorectal cancers", pp.2006
  48. Podhajcer OL, "The role of the matricellular protein SPARC in the dynamic interaction between the tumor and the host", Cancer Metastasis Reviews, 27(4), pp.691~705 (2008)
  49. Chen J, "SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer", PloS one, 7(8), pp.e42413~ (2012)
  50. Heinrich PC, "Principles of interleukin (IL)-6-type cytokine signalling and its regulation", The Biochemical Journal, 374(Pt 1), pp.1~20 (2003)
  51. Hutt JA, "Oncostatin M induces growth arrest of mammary epithelium via a CCAAT", enhancer-binding protein delta-dependent pathway, 2002, pp.1(8), 601~610
  52. Liu J, "Transcriptional suppression of synuclein gamma (SNCG) expression in human breast cancer cells by the growth inhibitory cytokine oncostatin M", Breast Cancer Research and Treatment, 62(2), pp.99~107 (2000)
  53. Garcia-Tunon I, "OSM, LIF, its receptors, and its relationship with the malignance in human breast carcinoma (in situ and in infiltrative)", Cancer Investigation, 26(3), pp.222~229 (2008)
  54. Douglas AM, "Oncostatin M induces the differentiation of breast cancer cells", International Journal of Cancer, 75(1), pp.64~73 (1998)
  55. Liu J, "Oncostatin M-specific receptor mediates inhibition of breast cancer cell growth and down-regulation of the c-myc proto-oncogene", Cell growth &differentiation : the molecular biology journal of the American Association for Cancer Research, 8(6), pp.667~676 (1997)

관련 논문AI추천

1회성 유산소 트레드밀 운동 강도가 수컷 마우스의 irisin 및 FNDC5 발현에 미치는 영향김재근, 남궁현, 이세원, 이지성한국운동생리학회 · 운동과학2018 · 제27권 제3호The benefits of exercise effect on cancer: A review이남주한국운동생리학회 · 운동과학2020 · 제29권 제1호운동과 암: 예방에서 치료까지?곽이섭, 서한교국민체육진흥공단 한국스포츠과학원 · 체육과학연구2020 · 제31권 제4호1-3기 대장암 생존자 증상 개선 운동 프로그램 효과 예비 검증: A Feasibility Study김남규, 김태일, 박동혁, 박지수, 변지용, 연수진, 이미경, 전용관, 지선하한국운동생리학회 · 운동과학2022 · 제31권 제1호흰쥐의 대장에서 운동강도에 따른 사이토카인 및 케모카인 유전자 발현김석동, 박한수, 채정룡, 최석준, 최은주한국운동생리학회 · 운동과학2009 · 제18권 제2호저산소 환경의 지구성 운동이 근육 위성세포의 활성 및 증식에 미치는 효과손희정, 임창현한국운동생리학회 · 운동과학2019 · 제28권 제1호

이 간행물의 다른 논문 (7건)

유방절제술로 발생된 상지 림프부종 환자의 치료적 운동 고찰최승준, 하경진pp. 69-7510,000 Lux의 풀스펙트럼 조사와 저강도 유산소 운동이 흰 쥐의 혈중 지질에 미치는 영향송광섭pp. 85-91Borg 10단계 비율척도의 운동 강도 설명력에 관한 연구정진욱, 이온pp. 92-99근수축에 의한 Nitric Oxide 생성과 미토콘드리아 생합성 조절체계에서 CaMKII의 작용박대령pp. 100-109골연령과 역연령(출생월)이 체육영재선발에 참여한 아동들의 체격 및 신체수행력에 미치는 영향박동호, 고진세, 김수진, 이상현pp. 110-119
원문 보기