본문 바로가기
KERD
논문KCI 등재

지구성 운동에 의한 AMPK 활성은 MEF2A 통하여 PPARβ/δ의발현을 조절한다.

AMPK Activation by Acute Endurance Exercise Controls PPARβ/δ Expression through MEF2A

김기진|안나영|고진호
한국운동생리학회|2016.05|운동과학 (Exercise Science)|제25권 제2호|pp. 127-133|DOI 10.15857/ksep.2016.25.2.127

0

조회

0

다운로드

1

인용

0

참고문헌

키워드

AMPK지구성 운동Endurance exerciseHDAC5PPARβ/δMEF2A

초록

PURPOSE: The aim of this study was to identify whether the increased PPARβ/δ gene expression induced by exercise is mediated by activation of AMPK and MEF2A. METHODS: To analyze PPARβ/δ mRNA, rat triceps muscles were dissected immediately after a single bout of swimming exercise, and constitutively active (CA) AMPK over expressed L6 rat skeletal muscle cells were harvested. MEF2A activation by AMPK was analyzed by a Gal4 and green fluorescence system in the L6 cells. Dominant negative AMPK was over expressed in rat tibialis anterior (TA) using electrical pulse-mediated gen transfer (electroporation [EPO]) method before 2 weeks swimming exercise. RESULTS: PPARβ/δ mRNA in rat TA muscle after exercise was increased by 5.5 fold compared with the sedentary muscle, and was increased by 6.7 fold in the CA-AMPK over expressed cells compared with the empty vector (EV) treated cells. MEF2A-Gal4 was activated by a 1.9 fold in the L6 cells transfected with CA-AMPK constructs. AMPK and HDAC5 phosphorylation, and PPARβ/δ expression were increased 3.2, 2.8, and 2.1 fold, respectively, by exercise in EV treated muscle, but were not increased by exercise in DNAMPK over expressed muscle. CONCLUSIONS: MEF2A activation by AMPK increases the PPARβ/δ expression in endurance exercised muscle.

Abstract

PURPOSE: The aim of this study was to identify whether the increased PPARβ/δ gene expression induced by exercise is mediated by activation of AMPK and MEF2A. METHODS: To analyze PPARβ/δ mRNA, rat triceps muscles were dissected immediately after a single bout of swimming exercise, and constitutively active (CA) AMPK over expressed L6 rat skeletal muscle cells were harvested. MEF2A activation by AMPK was analyzed by a Gal4 and green fluorescence system in the L6 cells. Dominant negative AMPK was over expressed in rat tibialis anterior (TA) using electrical pulse-mediated gen transfer (electroporation [EPO]) method before 2 weeks swimming exercise. RESULTS: PPARβ/δ mRNA in rat TA muscle after exercise was increased by 5.5 fold compared with the sedentary muscle, and was increased by 6.7 fold in the CA-AMPK over expressed cells compared with the empty vector (EV) treated cells. MEF2A-Gal4 was activated by a 1.9 fold in the L6 cells transfected with CA-AMPK constructs. AMPK and HDAC5 phosphorylation, and PPARβ/δ expression were increased 3.2, 2.8, and 2.1 fold, respectively, by exercise in EV treated muscle, but were not increased by exercise in DNAMPK over expressed muscle. CONCLUSIONS: MEF2A activation by AMPK increases the PPARβ/δ expression in endurance exercised muscle.

참고문헌 (43)

  1. Holloszy JO, "Regulation by exercise of skeletal muscle content of mitochondria and GLUT4", Journal of Physiology and Pharmacology, 59(Suppl 7), pp.5~18 (2008)
  2. Kelly DP, "Transcriptional regulatory circuits controlling mitochondrial biogenesis and function", Genes & Development, 18(4), pp.357~368 (2004)
  3. Lin J, "Metabolic control through the PGC-1 family of transcription coactivators", Cell metabolism, 1(6), pp.361~370 (2005)
  4. Scarpulla RC, "Nuclear control of respiratory gene expression in mammalian cells", Journal of Cellular Biochemistry, 97(4), pp.673~683 (2006)
  5. Wang, YX, "Regulation of muscle fiber type and running endurance by PPARdelta", PLoS Biology, 2(10), pp.e294~ (2004)
  6. 고진호, "지구성 운동에 의하여 증가된 PPARβ", δ는 NRF-1 promoter 활성을 통하여 생쥐 근육내 GLUT4 및 미토콘드리아 효소의 발현을 유도한다, 2015, pp.24(2), 153~160[DOI]
  7. 고진호, "PPARδ가 생쥐 골격근의 미토콘드리아 생합성에 미치는 영향", 운동과학, 23(4), pp.315~322 (2014), 한국운동생리학회
  8. 고진호, "PPARβ", δ 과발현은 PGC-1α ubiquitination의 감소를 통해 PGC-1α 단백질을 증가시킨다, 2015, pp.24(4), 407~414[DOI]
  9. 고진호, "PPARδ의 활성 또는 과발현은 PGC-1α 단백질의 분해를 지연시킨다", 운동과학, 24(1), pp.49~58 (2015), 한국운동생리학회[DOI]
  10. 정수련, "운동 중 골격근 내 PGC-1α 농도는 PPARβ", δ를 통한 후전사 기전에 의하여 조절된다: PPARβ, 운동과학, pp.2015, 24(3)[DOI]
  11. Potthoff MJ, "MEF2: a central regulator of diverse developmental programs", Development, 134(23), pp.4131~4140 (2007)
  12. McGee SL, "AMP-activated protein kinase regulates GLUT4 transcription by phosphorylating histone deacetylase 5", Diabetes, 57(4), pp.860~867 (2008)
  13. McKinsey TA, "Activation of the myocyte enhancer factor-2 transcription factor by calcium", calmodulin-dependent protein kinase-stimulated binding of 14-3-3 to histone deacetylase 5, 2000, pp.97(26), 14400~14405
  14. Hardie DG, "AMP-activated protein kinase: the energy charge hypothesis revisited", Bioessays, 23(12), pp.1112~1119 (2001)
  15. Hardie DG, "AMP-activated protein kinase - development of the energy sensor concept", The Journal of Physiology, 574(1), pp.7~15 (2006)
  16. Hardie DG, "AMP-activated protein kinase: a key system mediating metabolic responses to exercise", Medicine and Science in Sports and Exercise, 36(1), pp.28~34 (2004)
  17. Park SH, "Phosphorylation-activity relationships of AMPK and acetyl-CoA carboxylase in muscle", Journal of Applied Physiology, 92(6), pp.2475~2482 (2002)
  18. Vavvas D, "Contractioninduced changes in acetyl-CoA carboxylase and 5’-AMP-activated kinase in skeletal muscle", Journal of Biological Chemistry, 272(20), pp.13255~13261 (1997)
  19. Winder WW, "AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes", American Journal of Physiology-Endocrinology And Metabolism, 277(1 Pt 1), pp.E1~E10 (1999)
  20. Winder WW, "Inactivation of acetyl-CoA carboxylase and activation of AMP-activated protein kinase in muscle during exercise", American Journal of Physiology-Endocrinology And Metabolism, 270(2 Pt 1), pp.E299~E304 (1996)
  21. Wojtaszewski JF, "Insulin signaling and insulin sensitivity after exercise in human skeletal muscle", Diabetes, 49(3), pp.325~331 (2000)
  22. McGee SL, "Exercise‐induced histone modifications in human skeletal muscle", The Journal of Physiology, 587(24), pp.5951~5958 (2009)
  23. Mukwevho E, "Caffeine induces hyperacetylation of histones at the MEF2 site on the Glut4 promoter and increases MEF2A binding to the site via a CaMK-dependent mechanism", American Journal of Physiology-Endocrinology and Metabolism, 294(3), pp.E582~E588 (2008)
  24. Z. Gan, "The nuclear receptor PPAR", programs muscle glucose metabolism in cooperation with AMPK and MEF2, 2011, pp.25(24), 2619~2630[DOI]
  25. Akimoto T, "Real-time imaging of peroxisome proliferator-activated receptor-gamma coactivator-1alpha promoter activity in skeletal muscles of living mice", American Journal of Physiology-Cell Physiology, 287(3), pp.C790~C796 (2004)
  26. Terada S, "Effects of low-intensity prolonged exercise on PGC-1 mRNA expression in rat epitrochlearis muscle", Biochemical and Biophysical Research Communications, 296(2), pp.350~354 (2002)
  27. Terada S, "Effects of acute bouts of running and swimming exercise on PGC-1alpha protein expression in rat epitrochlearis and soleus muscle", American Journal of Physiology-Endocrinology and Metabolism, 286(2), pp.E208~E216 (2004)
  28. Lowry OH, "Protein measurement with the Folin phenol reagent", The Journal of Biological Chemistry, 193(1), pp.265~275 (1951)
  29. Potthoff MJ, "Histone deacetylase degradation and MEF2 activation promote the formation of slow-twitch myofibers", The Journal of Clinical Investigation, 117(9), pp.2459~2467 (2007)
  30. Chen ZP, "Effect of exercise intensity on skeletal muscle AMPK signaling in humans", Diabetes, 52(9), pp.2205~2212 (2003)
  31. Reznick RM, "The role of AMP‐activated protein kinase in mitochondrial biogenesis", The Journal of Physiology, 574(1), pp.33~39 (2006)
  32. Bronner M, "Kinase-independent transcriptional co-activation of peroxisome proliferator-activated receptor α by AMPactivated protein kinase", Biochemical Journal, 384(2), pp.295~305 (2004)
  33. Hong, YH, "AMP-activated protein kinase regulates HNF4alpha transcriptional activity by inhibiting dimer formation and decreasing protein stability", The Journal of Biological Chemistry, 278(30), pp.27495~27501 (2003)
  34. Jäger S, "AMP-activated protein kinase (AMPK) action in skeletal muscle via direct phosphorylation of PGC-1alpha", Proceedings of the National Academy of Sciences, 104(29), pp.12017~12022 (2007)
  35. Leff T, "AMP-activated protein kinase regulates gene expression by direct phosphorylation of nuclear proteins", Biochemical Society Transactions, 31(1), pp.224~227 (2003)
  36. Narkar VA, "AMPK and PPARδ Agonists Are Exercise Mimetics", Cell, 134(3), pp.405~415 (2008)
  37. Mu J, "A Role for AMP-Activated Protein Kinase in Contraction- and Hypoxia-Regulated Glucose Transport in Skeletal Muscle", Molecular Cell, 7(5), pp.1085~1094 (2001)
  38. Thomson DM, "Skeletal muscle and heart LKB1 deficiency causes decreased voluntary running and reduced muscle mitochondrial marker enzyme expression in mice. American Journal of Physiology", Endocrinology and Metabolism, 292(1), pp.E196~E202 (2007)
  39. Olson EN, "Calcineurin Signaling and Muscle Remodeling", Cell, 101(7), pp.689~692 (2000)
  40. Hood DA, "Invited Review: contractile activity-induced mitochondrial biogenesis in skeletal muscle", Journal of Applied Physiology, 90(3), pp.1137~1157 (2001)
  41. Berchtold MW, "Calcium ion in skeletal muscle: its crucial role for muscle function, plasticity, and disease", Physiological Reviews, 80(3), pp.1215~1265 (2000)
  42. Corcoran EE, "Defining Ca2+", calmodulin-dependent protein kinase cascades in transcriptional regulation, 2001, pp.276(5), 2975~2978
  43. Handschin C, "An autoregulatory loop controls peroxisome proliferator-activated receptor gamma coactivator 1alpha expression in muscle", PNAS, 100(12), pp.7111~7116 (2003)

관련 논문AI추천

지구성 운동에 의하여 증가된 PPARβ/δ는 NRF-1 promoter 활성을 통하여 생쥐 근육내 GLUT4 및 미토콘드리아 효소의 발현을 유도한다고진호, 김기진, 김상현, 정수련한국운동생리학회 · 운동과학2015 · 제24권 제2호일회성 지구성운동 시 MEF2를 통한 PPARδ의 GLUT4 생합성 조절고진호, 김기진, 김상현, 정수련한국운동생리학회 · 운동과학2015 · 제24권 제1호운동 중 골격근 내 PGC-1α 농도는 PPARβ/δ를 통한 후전사 기전에 의하여 조절된다: PPARβ/δ silence가 ubiquitin을 통한 PGC-1α 안정성에 미치는 영향고진호, 김기진, 정수련한국운동생리학회 · 운동과학2015 · 제24권 제3호PPARβ/δ 과발현은 PGC-1α ubiquitination의 감소를 통해 PGC-1α 단백질을 증가시킨다고진호, 김기진, 김홍수한국운동생리학회 · 운동과학2015 · 제24권 제4호운동에 의한 골격근 내 PGC-1α전사 조절 기전고진호, 김기진, 김상현, 김필상한국운동생리학회 · 운동과학2013 · 제22권 제3호식이섭취 형태와 일회성 운동이 골격근내 PPAR isoforms 유전자 발현에 미치는 영향김정규, 문희원, 박승한, 이장규, 이종삼, 임영조한국운동생리학회 · 운동과학2008 · 제17권 제4호

이 간행물의 다른 논문 (7건)

유방절제술로 발생된 상지 림프부종 환자의 치료적 운동 고찰최승준, 하경진pp. 69-75장기간의 운동이 대장암 유발 쥐의 SPARC와 OSM발현에 미치는 영향김준홍, 조은효, 전태원pp. 76-8410,000 Lux의 풀스펙트럼 조사와 저강도 유산소 운동이 흰 쥐의 혈중 지질에 미치는 영향송광섭pp. 85-91Borg 10단계 비율척도의 운동 강도 설명력에 관한 연구정진욱, 이온pp. 92-99근수축에 의한 Nitric Oxide 생성과 미토콘드리아 생합성 조절체계에서 CaMKII의 작용박대령pp. 100-109
원문 보기