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지구성 운동에 의하여 증가된 PPARβ/δ는 NRF-1 promoter 활성을 통하여 생쥐 근육내 GLUT4 및 미토콘드리아 효소의 발현을 유도한다

The increased PPARβ/δ by endurance exercise control MEF2A-GLUT4 and mitochodrial enzymes through NRF-1 promoter activity in mouse skeletal muscle

김상현|김기진|정수련|고진호
한국운동생리학회|2015.05|운동과학 (Exercise Science)|제24권 제2호|pp. 153-160|DOI 10.15857/ksep.2015.24.2.153

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참고문헌

키워드

지구성 운동Endurance exerciseGLUT4PPARβ/δNRF-1미토콘드리아 효소mitochondrial enzymes

초록

PURPOSE: The propose of this study was to evaluate whether PPARβ/δ can increase mitochondrial enzymes and GLUT4 through NRF-1 promoter activity. METHODS: To evaluate, PPARβ/δ and NRF-1 protein expression in mouse skeletal muscle, TA muscle were dissect at 24h after 10 day swimming exercise(Ex). To evaluate PPARβ/δ effects on gene regulation, PPARβ/δ was overexpressed in mouse TA muscle using electrical pulse-mediated gen transfer(electroporation; EPO) method. We conducted chip and promoter reporter assay to confirm of PPARβ/δ can bind NRF-1 promoter and activates NRF-1 promoter in mouse muscle cell. RESULTS: PPARβ/δ and NRF-1 protein expression in skeletal muscle of 10 day Ex group were increased 2.2 and 2.7 fold respectively when compare to non-exercise muscle. PGC-1α protein expression in PPARβ/δ overexpression skeletal muscle were not different, but NRF-1, MEF2A, GLUT4, ATP syn and Cyt C expression were increased 2.2, 2.3, 2.1, 2 and 1.7 fold respectively when compare to empty vector(EV) treated muscle. Our ChiP and promoter activity assay result show that NRF-1 promoter has a PPARβ/δ binding site(PPRE) and PPARβ/δ can activates NRF-1 promoter through the PPRE. CONCLUSIONS: The increased PPARβ/δ by endurance exercise can control MEF2A-GLUT4 and mitochodrial enzymes such as ATP syn and Cyt C through NRF-1 promoter activity.

Abstract

PURPOSE: The propose of this study was to evaluate whether PPARβ/δ can increase mitochondrial enzymes and GLUT4 through NRF-1 promoter activity. METHODS: To evaluate, PPARβ/δ and NRF-1 protein expression in mouse skeletal muscle, TA muscle were dissect at 24h after 10 day swimming exercise(Ex). To evaluate PPARβ/δ effects on gene regulation, PPARβ/δ was overexpressed in mouse TA muscle using electrical pulse-mediated gen transfer(electroporation; EPO) method. We conducted chip and promoter reporter assay to confirm of PPARβ/δ can bind NRF-1 promoter and activates NRF-1 promoter in mouse muscle cell. RESULTS: PPARβ/δ and NRF-1 protein expression in skeletal muscle of 10 day Ex group were increased 2.2 and 2.7 fold respectively when compare to non-exercise muscle. PGC-1α protein expression in PPARβ/δ overexpression skeletal muscle were not different, but NRF-1, MEF2A, GLUT4, ATP syn and Cyt C expression were increased 2.2, 2.3, 2.1, 2 and 1.7 fold respectively when compare to empty vector(EV) treated muscle. Our ChiP and promoter activity assay result show that NRF-1 promoter has a PPARβ/δ binding site(PPRE) and PPARβ/δ can activates NRF-1 promoter through the PPRE. CONCLUSIONS: The increased PPARβ/δ by endurance exercise can control MEF2A-GLUT4 and mitochodrial enzymes such as ATP syn and Cyt C through NRF-1 promoter activity.

참고문헌 (30)

  1. Akimoto T, "Real- time imaging o f peroxisome proliferator-activated receptor-gamma coactivator-1alpha promoter activity in skeletal muscles of living mice. American Journal of Physiology", Cell Physiology, 287(3), pp.790~796 (2004)
  2. Burkart EM, "Nuclear receptors PPARβ", δ and PPARα direct distinct metabolic regulatory programs in the mouse heart, 2007, pp.117(12), 3930~3939
  3. Evans MJ, "Interaction of nuclear factors with multiple sites in the somatic cytochrome c promoter. Characterization of upstream NRF-1, ATF, and intron Sp1recognition sequences", The Journal of Biological Chemistry, 264(24), pp.14361~14368 (1989)
  4. Finck BN, "A potential link between muscle peroxisome proliferator-activated receptor-alpha signaling and obesityrelated diabetes", Cell Metabolism, 1(2), pp.133~144 (2005)
  5. Finck BN, "The cardiac phenotype induced by PPARalpha overexpression mimics that caused by diabetes mellitus", The Journal of Clinical Investigation, 109(1), pp.121~130 (2002)
  6. Fritz T, "Low-intensity exercise increases skeletal muscle protein expression of PPARdelta and UCP3 in type 2 diabetic patients", Diabetes, 2006, pp.22(6), 492~498
  7. Gan Z, "The nuclear receptor PPARβ", δprograms muscle glucose metabolism in cooperation with AMPK and MEF2, 2011, pp.25(24), 2619~2630
  8. Garcia-Roves P, "Raising plasma fatty acid concentration induces increased biogenesis of mitochondria in skeletal muscle", Proceedings of the National Academy of Sciences, 104(25), pp.10709~10713 (2007)
  9. Greene NP, "Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight", obese men and women, 2012, pp.303(10), 1212~1221
  10. Hancock CR, "High-fat diets cause insulin resistance despite an increase in muscle mitochondria", Proceedings of the National Academy of Sciences, 105(22), pp.7815~7820 (2008)
  11. Holloszy JO, "Regulation by exercise of skeletal muscle content of mitochondria and GLUT4", Journal of Physiology and Pharmacology : an Official Journal of the Polish Physiological Society, 59(Suppl 7), pp.5~18 (2008)
  12. 김상현, "일회성 지구성운동 시 MEF2를 통한 PPARδ의 GLUT4 생합성 조절", 운동과학, 24(1), pp.59~66 (2015), 한국운동생리학회[DOI]
  13. Kelly DP, "Transcriptional regulatory circuits controlling mitochondrial biogenesis and function", Genes & Development, 18(4), pp.357~368 (2004)
  14. 고진호, "PPARδ가 생쥐 골격근의 미토콘드리아 생합성에 미치는 영향", 운동과학, 23(4), pp.315~322 (2014), 한국운동생리학회
  15. Lin J, "Metabolic control through the PGC-1 family of transcription coactivators", Cell Metabolism, 1(6), pp.361~370 (2005)
  16. Lowry OH, "Protein measurement with the Folin phenol reagent", The Journal of Biological Chemistry, 193(1), pp.265~275 (1951)
  17. McGee SL, "Exercise increases MEF2- and GEF DNA-binding activity in human skeletal muscle", FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology, 20(2), pp.348~349 (2006)
  18. Michael LF, "Restoration of insulin-sensitive glucose transporter(GLUT4)gene expression in muscle cells by the transcriptional coactivator PGC-1", Proceedings of the National Academy of Sciences, 98(7), pp.3820~3825 (2001)
  19. Mora S, "The MEF2A isoform is required for striated muscle-specific expression of the insulin-responsive GLUT4 glucose transporter", The Journal of Biological Chemistry, 275(21), pp.16323~16328 (2000)
  20. Perry CGR, "Repeated transient mRNA bursts precede increases in transcriptional and mitochondrial proteins during training in human skeletal muscle", The Journal of Physiology, 588(Pt 23), pp.4795~4810 (2010)
  21. Ploug T, "Effect of endurance training on glucose transport capacity and glucose transporter expression in rat skeletal muscle. American Journal of Physiology", Endocrinology and Metabolism, 259(6), pp.778~786 (1990)
  22. Ramachandran B, "Nuclear respiratory factor 1 controls myocyte enhancer factor 2A transcription to provide a mechanism for coordinate expression of respiratory chain subunits", The Journal of Biological Chemistry, 283(18), pp.11935~11946 (2008)
  23. Ren JM, "Overexpression of Glut4 protein in muscle increases basal and insulin-stimulated whole body glucose disposal in conscious mice", The Journal of Clinical Investigation, 95(1), pp.429~432 (1995)
  24. Scarpulla RC, "Transcriptional activators and coactivators in the nuclear control of mitochondrial function in mammalian cells", Gene, 286, pp.81~89 (2002)
  25. Scarpulla RC, "Nuclear control of respiratory gene expression in mammalian cells", Journal of Cellular Biochemistry, 97(4), pp.673~683 (2006)
  26. Terada S, "Effects of acute bouts of running and swimming exercise on PGC-1alpha protein expression in rat epitrochlearis and soleus muscle. American Journal of Physiology", Endocrinology and Metabolism, 286(2), pp.208~216 (2004)
  27. Terada S, "Effects of low-intensity prolonged exercise on PGC-1mRNA expression in rat epitrochlearis muscle", Biochemical and Biophysical Research Communications, 296(2), pp.350~354 (2002)
  28. Wang YX, "Peroxisome-proliferator-activated receptor delta activates fat metabolism to prevent obesity", Cell, 113(2), pp.159~170 (2003)
  29. Wang YX, "Regulation of muscle fiber type and running endurance by PPARdelta", PLoS Biology, 2(10), pp.e294~ (2004)
  30. 김상현, "지구성 운동 시 NT-PGC-1α가 흰쥐 골격근 내 미토콘드리아 생합성에 미치는 영향", 운동과학, 21(4), pp.435~443 (2012), 한국운동생리학회

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