저항성 운동이 OLETF 쥐 시상하부 Leptin receptor (Ob-R), GLUT2 mRNA 및 골격근 GLUT4 mRNA 발현에 미치는 영향
Effects of Resistance Exercise Training on Leptin Receptor (Ob-R), GLUT2 mRNA of the Hypothalamus and GLUT4 mRNA Expression of the Skeletal Muscle in OLETF Rats
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PURPOSE: The hypothalamus plays a central role in integrating hormonal and nutritional signals from the periphery and modulating food intake, energy balance. However, obesity and type 2 diabetes cause dysfunction of hypothalamus. This study was to investigate the effects of 8 weeks resistance exercise training on leptin receptor, GLUT2 mRNA of the hypothalamus and skeletal muscle GLUT4 mRNA expression in OLETF rats. METHODS: Thirty male OLETF rats were randomly divided into three groups: LETO (CON, n=10), OLETF (OLETF+CON, n=10), OLETF+resistance exercise (OLETF+EXE, n=10). Exercise group conducted gradual ladder climbing exercise 3 days per week for 8 weeks. RESULTS: The results of this study was following; In the hypothalamus, leptin receptor (Ob-R) and GLUT2 mRNA expression were significantly increased in OLETF+EXE group compared with OLETF group (p<.05). Activation of AMPK was significantly decreased in OLETF+EXE group compared to OLETF group (p<.05). GLUT4 mRNA expression of skeletal muscle was significantly increased in OLETF+EXE group compared with OLETF group (p<.05), and HOMA-IR index also was significantly lower in OLETF+EXE group than OLETF group (p<.05). CONCLUSIONS: These results showed that 8 weeks resistance exercise training can improve leptin receptor mRNA expression and glucose sensing in hypothalamic. Also, these effects may contribute to energy homeostasis in peripheral such as skeletal muscle.
Abstract
PURPOSE: The hypothalamus plays a central role in integrating hormonal and nutritional signals from the periphery and modulating food intake, energy balance. However, obesity and type 2 diabetes cause dysfunction of hypothalamus. This study was to investigate the effects of 8 weeks resistance exercise training on leptin receptor, GLUT2 mRNA of the hypothalamus and skeletal muscle GLUT4 mRNA expression in OLETF rats. METHODS: Thirty male OLETF rats were randomly divided into three groups: LETO (CON, n=10), OLETF (OLETF+CON, n=10), OLETF+resistance exercise (OLETF+EXE, n=10). Exercise group conducted gradual ladder climbing exercise 3 days per week for 8 weeks. RESULTS: The results of this study was following; In the hypothalamus, leptin receptor (Ob-R) and GLUT2 mRNA expression were significantly increased in OLETF+EXE group compared with OLETF group (p<.05). Activation of AMPK was significantly decreased in OLETF+EXE group compared to OLETF group (p<.05). GLUT4 mRNA expression of skeletal muscle was significantly increased in OLETF+EXE group compared with OLETF group (p<.05), and HOMA-IR index also was significantly lower in OLETF+EXE group than OLETF group (p<.05). CONCLUSIONS: These results showed that 8 weeks resistance exercise training can improve leptin receptor mRNA expression and glucose sensing in hypothalamic. Also, these effects may contribute to energy homeostasis in peripheral such as skeletal muscle.
참고문헌 (41)
- Malin SK, "Type 2 diabetes treatment in the patient with obesity", Endocrinol Metab Clin North Am, 45(3), pp.553~564 (2016)
- Leitner DR, "Obesity and type 2 diabetes : two diseases with a need for combined treatment strategies-EASO can lead the way", Obes Facts, 10(5), pp.483~492 (2017)
- Smokovski I, "Diabetes care in republic of macedonia : challenges and opportunities", Ann Glob Health, 81(6), pp.792~802 (2015)
- Klok MD, "The role of leptin and ghrelin in the regulation of food intake and body weight in humans : a review", Obes Rev, 8(1), pp.21~34 (2007)
- Rosenbaum M, "20 years of leptin: role of leptin in energy homeostasis in humans", J Endocrinol, 223(1), pp.T83~96 (2014)
- Coppari R, "Leptin revisited : its mechanism of action and potential for treating diabetes", Nat Rev Drug Discov, 11(9), pp.692~708 (2012)
- Tortoriello DV, "Increased expression of hypothalamic leptin receptor and adiponectin accompany resistance to dietary-induced obesity and infertility in female C57BL", 6J mice, 2007, pp.31(3), 395~402
- Jordan SD, "Sensing the fuels : glucose and lipid signaling in the CNS controlling energy homeostasis", Cell Mol Life Sci, 67(19), pp.3255~3273 (2010)
- Fuente-Martin E, "Leptin regulates glutamate and glucose transporters in hypothalamic astrocytes", J Clin Invest, 122(11), pp.3900~3913 (2012)
- Penicaud L, "Brain glucose sensing : a subtle mechanism", Curr Opin Clin Nutr Metab Care, 9(4), pp.458~462 (2006)
- Su H, "Glucose enhances leptin signaling through modulation of AMPK activity", PLoS One, 7(2), pp.e31636~ (2012)
- Andersson U, "AMPactivated protein kinase plays a role in the control of food intake", J Biol Chem, 279(13), pp.12005~12008 (2004)
- Huo L, "Leptin-dependent control of glucose balance and locomotor activity by POMC neurons", Cell Metab, 9(6), pp.537~547 (2009)
- Yi SS, "Effects of exercise on brain functions in diabetic animal models", World J Diabetes, 6(4), pp.583~597 (2015)
- Bi S, "Running wheel activity prevents hyperphagia and obesity in Otsuka long-evans Tokushima Fatty rats : role of hypothalamic signaling", Endocrinology, 146(4), pp.1676~1685 (2005)
- Laing BT, "Voluntary exercise improves hypothalamic and metabolic function in obese mice", J Endocrinol, 229(2), pp.109~122 (2016)
- Shapiro A, "The act of voluntary wheel running reverses dietary hyperphagia and increases leptin signaling in ventral tegmental area of aged obese rats", Gerontology, 57(4), pp.335~342 (2011)
- Yi X, "Effects of acute exercise and chronic exercise on the liver leptin-AMPK-ACC signaling pathway in rats with type 2 diabetes", J Diabetes Res, 2013, pp.946432~ (2013)
- Araujo PCO, "Resistance exercise reduces memory impairment induced by monosodium glutamate in male and female rats", Exp Physiol, 102(7), pp.845~853 (2017)
- de Almeida AA, "Resistance exercise reduces seizure occurrence, attenuates memory deficits and restores BDNF signaling in rats with chronic epolepsy", Neurochem Res, 42(4), pp.1230~1239 (2017)
- Lee SH, "Viral expression of insulin-like growth factor-Ι enhances muscle hypertrophy in resistancetrained rat", J Appl Physiol, 96(3), pp.1097~1104 (2004)
- Yang HJ, "Combination of aronia, red ginseng, shiitake mushroom and nattokinase potentiated insulin secretion and reduced insulin resistance with improving gut microbiome dysbiosis in insulin deficient type 2 diabetic rats", Nutrients, 10(7), pp.E948~ (2018)
- Morton GJ, "Central nervous system control of food intake and body weight", Nature, 443(7109), pp.289~295 (2006)
- Yi XJ, "Exercise on the sexual development and the fat leptin receptor mRNA expression in the high-fat die female rats", Zhongguo Ying Yong Sheng Li Xue Za Zhi, 25(4), pp.454~456 (2009)
- Fuentes T, "Leptin receptor 170 kDa(OB-R170)protein expression is reduced in obese human skeletal muscle : a potential mechanism of leptin resistance", Exp Physiol, 95(1), pp.160~171 (2010)
- Thivel D, "The effects of imposed sedentary behavior and exercise on energy intake in adolescents with obesity", J Dev Behav Pediatr, 34(8), pp.616~622 (2013)
- Friedman JM, "Leptin at 14 y of age: an ongoing story", Am J Clin Nutr, 89(3), pp.973~979 (2009)
- Keen-Rhinehart E, "AAV-mediated leptin receptor installation improves energy balance and the reproductive status of obese female Koletsky rats", Peptide, 26(12), pp.2567~2578 (2005)
- Zhao J, "Endurance exercise is a leptin signaling mimetic in hypothalamus of Wistar rats", Lipids Health Dis, 10, pp.225~ (2011)
- Patterson CM, "Three weeks of postweaning exercise in DIO rats produces prolonged increases in central leptin sensitivity and signaling", Am J Physiol Regul Integr Comp Physiol, 296(3), pp.R537~548 (2009)
- Millán C, "Glial glucokinase expression in adult and post-natal development of the hypothalamic region", ASN Neuro, 2(3), pp.e00035~ (2010)
- Marín-Juez R, "GLUT2-mediated glucose uptake and availability are required for embryonic brain development in zebrafish", J Cereb Blood Flow Metab, 35(1), pp.74~85 (2015)
- Bady I, "Evidence from glut2-null mice that glucose is a critical physiological regulator of feeding", Diabetes, 55(4), pp.988~995 (2006)
- Park S, "Chronic activation of central AMPK attenuates glucose-stimulated insulin secretion and exacerbates hepatic insulin resistance in diabetic rats", Brain Res Bull, 108, pp.18~26 (2014)
- Auer MK, "Effects of a high-caloric diet and physical exercise on brain metabolite levels : a combined proton MRS and histologic study", J Cereb Blood Flow Metab, 35(4), pp.554~564 (2015)
- Laing BT, "Voluntary exercise improves hypothalamic and metabolic function in obese mice", J Endocrinol, 229(2), pp.109~122 (2016)
- Motiani KK, "Two weeks of moderate-intensity continuous training, but not highintensity interval training, increases insulin-stimulated intestinal glucose uptake", J Appl Physiol, 122(5), pp.1188~1197 (2017)
- Toda C, "Extracellular signal-regulated kinase in the ventromedial hypothalamus mediates leptin-induced glucose uptake in red-type skeletal muscle", Diabetes, 62(7), pp.2295~2307 (2013)
- Richter EA, "Exercise, GLUT4, and skeletal muscle glucose uptake", Physiol Rev, 93(3), pp.993~1017 (2013)
- Cunha VN, "Role of exercise intensity on GLUT4 content, aerobic fitness and fasting plasma glucose in type 2 diabetic mice", Cell Biochem Funct, 33(7), pp.435~442 (2015)
- 김지연, "The Preventive Effects of 8 Weeks of Resistance Training on Glucose Tolerance and Muscle Fiber Type Composition in Zucker Rats", Diabetes and Metabolism Journal, 39(5), pp.424~433 (2015), 대한당뇨병학회[DOI]
