다양한 저산소환경에서의 최대운동 시 ACE 유전자 다형성에 따른 생리적 변인들의 반응
The responses of physiological factors during graded maximal exercise(GXT) according to ACE gene polymorphism under various altitude hypobaric hypoxia (VAHH)
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본 연구는 해수면(100m) 및 다양한 저산소환경(1,300m, 1,800m, 2,650m)에서의 최대운동 시 ACE 유전자 다형성에 따른 인체의 생리적인 반응을 통하여 지구성 운동수행능력에 미치는 유전적 소인의 차이를 알아보는데 있었다. I대학교의 체육전공 대학생 12명(II 타입 6명, DD 타입 6명)을 대상(24.08±1.98yrs, 177.13±4.22㎝ 75.85±8.31㎏, 15.89 ±2.36%fat)으로 저압·저산소실에서 자전거 에르고미터를 이용하여 최대운동부하검사를 실시하였다. 혈액샘플은 운동전, 운동직후, 회복 30분, 회복 60분에 주정맥에서 10㎖씩 각각 채혈하였다. 헤마토크릿(Hct)을 제외한 모든 혈액 변인들은 혈장량의 변화(%)를 이용하여 보정하였다. 이원반복변량분석으로 생리적 및 혈액 변인들의 차이를 분석하였다. 본 연구결과로서, 1. 다양한 저산소환경에서 ACE 유전자다형성에 따라 VO2max, 운동지속시간, SaO2, 망상적혈구, 적혈구, Hct, Hb, EPO 및 알도스테론에서 저산소환경별 주효과에서 유의한 차이는 나타나지 않았다. 2. RBC, Hct 및 Hb은 2,650m에서 유의한 상호작용효과가 나타났다(p<.025, p<.013, p<.017). 3. 알도스테론은 1,800m에서 유의한 상호작용효과가 나타났다(p<.025). 4. ACE는 1,300m, 1,800m 및 2,650m에서 DD 타입이 II 타입에 비해 유의하게 높았다. 결론적으로, II 타입은 알도스테론의 분비를 통한 체수분 보유로 혈액량을 증가시켜 혈액공급을 유지하는 반면, DD 타입은 ACE분비로 인한 혈관수축으로 혈액공급을 유지하는 것으로 판단되며, 이러한 ACE 유전자형에 따른 RAA 시스템의 기능적 차이는 2,650m의 저산소 환경에서 더욱 두드러지게 나타난다.
Abstract
The purpose of this study was to observe the difference of gene factor via physiological responses according to the ACE genotype after the GXT under VAHH. 12 healthy college aged-men(II type 6, DD type 6 :24.08±1.98yrs, 177.13±4.22㎝, 75.85±8.31㎏, 15.89 ±2.36%fat) done GXT using bicycle ergometer in four different Hypobaric Hypoxic Chamber(100m, 1,300m, 1,800m and 2,650m). For each trial, blood samples were taken at pre-exercise, immediately post-exercise, 30min after exercise and 60 min after exercise. All samples were corrected for plasma volume changes and compared to pre-exercise except Hct. For the analysis of the differences between groups, two-way repeated measure ANOVA was used. The results are summarized as the follows. 1. VO2max, exercise duration, SaO2, reticulocyte, red blood cells, Hct, Hb, EPO and aldosterone showed no significant difference according to ACE gene polymorphism. 2. The interaction effect between types and time showed a signigicant difference in RBC, Hct, and Hb under 2,650m(p<.025, p<.013, p<.017). 3. The interaction difference in aldosterone under 1,800m(p<.025). 4. ACE showed significant difference under 1,300m, 1,800m and 2,650m, especially DD type was higher than II type. To conclude II type keeps the circulation of blood by aldosterone secretion, but DD type keeps the circulation of blood by ACE secretion. Such RAA system functional difference as showed remarkably under 2,650m.
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