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PPARδ의 활성 또는 과발현은 PGC-1α 단백질의 분해를 지연시킨다

PPARδ activation or overexpression attenuates PGC-1α protein degradation

김홍수|김기진|안나영|고진호
한국운동생리학회|2015.02|운동과학 (Exercise Science)|제24권 제1호|pp. 49-58|DOI 10.15857/ksep.2015.24.1.49

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참고문헌

키워드

PPARδPGC-1α지구성 운동Endurance exercise미토콘드리아 효소mitochondrial enzymes

초록

본 연구는 PPARδ가 1회 지구성 운동 후 증가한 PGC-1α의 단백질 감소를 지연시킬 수 있는지 확인하는 것이다. 2주간 고지방식이를 이용한 PPARδ의 활성과 전기자극 유전자 전이를 이용하여 골격근에 PPARδ의 과발현을 유도하였다. 1회 장시간 지구성 운동(Ex 그룹)을 실시한 후 24h와 54h에 샘플링 하였다. 채집된 근육에서 PGC-1α, cytochrome c oxidase subunit Ⅳ(COXⅣ) 그리고 succinate-ubiquinone oxidoreductase(SUO)의 발현을 분석하였다. 모든 운동그룹은 좌업그룹보다 PGC-1α 및 미토콘드리아 효소가 유의하게 증가하였다. PGC-1α가 최고치를 이룬 후 54h post-Ex Chow그룹은 24h post-Ex Chow 그룹보다 PGC-1α가 60% 감소하였으나, 54h post-Ex의 HFD 그룹은 24h post-Ex HFD 그룹과 차이가 없었다. 54h post-Ex의 HFD 그룹은 Chow 그룹보다 약 1.4배 PGC-1α의 단백질 수준이 높았다. 54h post-Ex의 EV 그룹은 24h post-Ex EV 그룹보다 PGC-1α 단백질이 75% 감소한 반면 54h post-Ex의 PPARδ 그룹은 24h post-Ex PPARδ 보다 약 1% 감소하였다. 54h post-Ex의 PPARδ 그룹 PGC-1α는 EV 그룹보다 1.9배(p<0.001) 높았다. 54h post-Ex PPARδ 그룹의 COX Ⅳ와 SUO는 54h post-Ex EV 그룹보다 각각 1.4배와 1.94배 높았다. PPARδ의 활성 또는 과발현은 운동 후 증가된 PGC-1α가 빠르게 감소하는 것을 지연시키며, 동시에 미토콘드리아 효소의 수준도 높게 유지시킬 수 있다.

Abstract

"PURPOSE: The purpose of this study was to evaluate whether PPARδattenuates PGC-1α protein degradation in mouse skeletal muscle after a single bout of swimming exercise (Ex). METHODS: PPARδactivation in mouse skeletal muscle was achieved by high fat diet (HFD) for 2 weeks. PPARδoverexpression in mouse skeletal muscle was achieved by electroporation(EPO). To evaluate PGC-1α, cytochrome c oxidase subunit Ⅳ (COXⅣ) and succinate-ubiquinone oxidoreductase (SUO) protein in mouse skeletal muscle, tibialis anterior (TA) muscle were dissected at 24 h or 54 h after a single bout of swimming exercise. RESULTS: PGC-1α and mitochondrial enzyme in all exercised muscle were significantly increased by a single bout of swimming exercise when compare to non-exercised muscle. PGC-1α expression in 54 h post-Ex Chow group was decreased 60% when compare to 24 h post-Ex Chow group, but 54 h post-Ex HFD group was not significantly decrease when compare to 24 h post-Ex HFD. PGC-1αexpression in 54 h post-Ex HFD group was higher 1.4 fold than 54 h post-Ex Chow group. PGC-1α expression in 54 h post-Ex EV group was decreased 75% when compare to 24 h post-Ex Ev group, but 54 h post-Ex PPARδ group was decrease 1% when compare to 24 h post-Ex PPARδ. PGC-1α expression in 54 h post-Ex PPARδ group was 1.9 fold higher than 54 h post-Ex EV group. COXⅣ와 SUO expression in 54 h post-Ex PPARδ group were 1.4 and 1.94 fold higher than 54 h post-Ex EV group. CONCLUSIONS: This study demonstrated that PPARδ activation or overexpression attenuate PGC-1α protein degradation in skeletal muscle after a single bout of swimming exercise that causes a more increase mitochondrial biogenesis."

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