수소수 음용이 운동에 의해 생성된 생체 내 ROS 제거 효능에 미치는 영향
Removal Effect of Hydrogen Water Drinking on Exercise-induced Production of Reactive Oxygen Species in Adult Men and Women
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
PURPOSE: This study aimed to investigate whether hydrogen (H2) water drinking reduced oxidative damage products [8-hydroxy- 2-deoxyguanosine (8-oxo-dG), malondialdehyde (MDA), L-lactate] produced in blood after exercise-induced oxidative stress. METHODS: Men (n=7) and women (n=8) [systolic blood pressure (BP): 90-150 mmHg, diastolic BP: 60-100 mmHg] were recruited. Exercise was performed for 20 minutes (8 km/hr) using treadmill. Placebo or test group drunk water and hydrogen water (1.2-1.5 ppm, 1,000 mL), respectively, right after exercise. Three days after, the same experiment was performed in the cross-over manner. Blood samples were obtained 30 minutes before exercise, right after exercise, and 30, 60, 120 minutes after exercise. 8-oxo-dG, MDA and L-lactate in serum were determined using ELISA kit. RESULTS: The blood levels of 8-oxo-dG were significantly increased after exercise (from 381±9.4 to 524±14.9 ng/mL for male; from 367±13.5 to 509±10.7 ng/mL for female). In the placebo group, the increased 8-oxo-dG at each time was not significantly different from the peak level induced by the exercise except that at 120 minutes in female. In the test group, its levels at each time were significantly lower than that in the peak level. The effect of hydrogen water was more pronounced in female than in male. The results obtained with MDA were quite similar to those with 8-oxo-dG. The L-lactate levels were not significant statistically in both groups. CONCLUSIONS: The data suggest that the one time drinking of hydrogen water may suppress the exercise-induced oxidative stress.
Abstract
PURPOSE: This study aimed to investigate whether hydrogen (H2) water drinking reduced oxidative damage products [8-hydroxy- 2-deoxyguanosine (8-oxo-dG), malondialdehyde (MDA), L-lactate] produced in blood after exercise-induced oxidative stress. METHODS: Men (n=7) and women (n=8) [systolic blood pressure (BP): 90-150 mmHg, diastolic BP: 60-100 mmHg] were recruited. Exercise was performed for 20 minutes (8 km/hr) using treadmill. Placebo or test group drunk water and hydrogen water (1.2-1.5 ppm, 1,000 mL), respectively, right after exercise. Three days after, the same experiment was performed in the cross-over manner. Blood samples were obtained 30 minutes before exercise, right after exercise, and 30, 60, 120 minutes after exercise. 8-oxo-dG, MDA and L-lactate in serum were determined using ELISA kit. RESULTS: The blood levels of 8-oxo-dG were significantly increased after exercise (from 381±9.4 to 524±14.9 ng/mL for male; from 367±13.5 to 509±10.7 ng/mL for female). In the placebo group, the increased 8-oxo-dG at each time was not significantly different from the peak level induced by the exercise except that at 120 minutes in female. In the test group, its levels at each time were significantly lower than that in the peak level. The effect of hydrogen water was more pronounced in female than in male. The results obtained with MDA were quite similar to those with 8-oxo-dG. The L-lactate levels were not significant statistically in both groups. CONCLUSIONS: The data suggest that the one time drinking of hydrogen water may suppress the exercise-induced oxidative stress.
참고문헌 (32)
- Ohsawa I, "Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals", Nat Med, 13(6), pp.688~694 (2007)
- Hayashida K, "Hydrogen inhalation during normoxic resuscitation improves neurological outcome in a rat model of cardiac arrest independently of targeted temperature management", Circulation, 130(24), pp.2173~2180 (2014)
- Ohta S, "Molecular hydrogen as a novel antioxidant : overview of the advantages of hydrogen for medical applications", Methods Enzymol, 555, pp.289~317 (2015)
- Thirupathi A, "Effects of reactive oxygen species and interplay of antioxidants during physical exercise in skeletal muscles", J Physiol Biochem, 74(3), pp.359~367 (2018)
- Radak Z, "Exercise, oxidants, and antioxidants change the shape of the bell-shaped hormesis curve", Redox Biol, 12, pp.285~290 (2017)
- Aoki K, "Pilot study : effects of drinking hydrogen-rich water on muscle fatigue caused by acute exercise in elite athletes", Med Gas Res, 2, pp.12~ (2012)
- Da Ponte A, "Effects of hydrogen rich water on prolonged intermittent exercise", J Sports Med Phys Fitness, 58(5), pp.612~621 (2018)
- Ostojic SM, "Hydrogen-rich water affected blood alkalinity in physically active men", Res Sports Med, 22(1), pp.49~60 (2014)
- Tsubone H, "Effect of treadmill exercise and hydrogen-rich water intake on serum oxidative and anti-oxidative metabolites in serum of thorough bred horses", J Equine Sci, 24(1), pp.1~8 (2013)
- Svoboda P, "Neopterin, a marker of immune response, and 8-hydroxy-2’-deoxyguanosine, a marker of oxidative stress, correlate at high age as determined by automated simultaneous high-performance liquid chromatography analysis of human urine", Anal Biochem, 383(2), pp.236~242 (2008)
- 석민화, "16주 복합운동 프로그램이 중년 당뇨 여성의 혈중 지질과 미토콘드리아 DNA 손상에 미치는 영향", 운동과학, 21(2), pp.201~212 (2012), 한국운동생리학회[DOI]
- Weber D, "Oxidative stress markers and micronutrients in maternal and cord blood in relation to neonatal outcome", Eur J Clin Nutr, 68(2), pp.215~222 (2014)
- 김태호, "일회성 혈류제한 저항성 운동이 성인남성의 대퇴둘레, 젖산, CK 및 산화스트레스에 미치는 영향", 운동과학, 27(1), pp.50~61 (2018), 한국운동생리학회[DOI]
- 김창선, "유산소성 및 무산소성 최대운동 후 혈중 젖산 생성 및 제거율 비교", 운동과학, 26(4), pp.239~247 (2017), 한국운동생리학회[DOI]
- Singh A, "Podophyllotoxin and rutin modulates ionizing radiation-induced oxidative stress and apoptotic cell death in mice bone marrow and spleen", Front Immunol, 8, pp.183~ (2017)
- Yang L, "PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis", Nat Commun, 5, pp.4436~ (2014)
- Kapelouzou A, "Serum and tissue biomarkers in aortic stenosis", Glob Cardiol Sci Pract, 4, pp.49~ (2015)
- Hayashida K, "Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury", Biochem Biophys Res Commun, 73(1), pp.30~35 (2008)
- Shi J, "Hydrogen saline is protective for acute lung ischaemia", reperfusion injuries in rats, 2012, pp.21(9), 556~563
- Fukuda K1, "Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia", reperfusion through reducing oxidative stress, 2007, pp.361(3), 670~674
- Shingu C1, "Hydrogen-rich saline solution attenuates renal ischemia-reperfusion injury", J Anesth, 24(4), pp.569~574 (2010)
- Fu Y1, "Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson’s disease", Neurosci Lett, 453(2), pp.81~85 (2009)
- Nakashima-Kamimura N, "Molecular hydrogen alleviates nephrotoxicity induced by an anti-cancer drug cisplatin without compromising anti-tumor activity in mice", Chemother Pharmacol, 64(4), pp.753~761 (2009)
- Kajiya M, "Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate", Biochem Biophys Res Commun, 386(1), pp.11~15 (2009)
- Kajiyama S, "Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance", Nutr Res, 28(3), pp.137~143 (2008)
- Nakao A, "Effectiveness of hydrogen rich water on antioxidant status of subjects with potential metabolic syndrome-an open label pilot study", J Clin Biochem Nutr, 46(2), pp.140~149 (2010)
- Nakayama M, "A novel bioactive haemodialysis system using dissolved dihydrogen(H2)produced by water electrolysis : clinical trial", Nephro Dial Transplat, 583(13), pp.3026~3033 (2010)
- Ono H, "Improved brain MRI indices in the acute brain stem infarct sites treated with hydroxyl radical scavengers, Edaravone and hydrogen, as compared to Edaravone alone. A non-controlled study", Med Gas Res, 1(1), pp.12~ (2011)
- Ito M, "Open-label trial and randomized, double-blind, placebo-controlled, crossover trial of hydrogen-enriched water for mitochondrial and inflammatory myopathies", Med Gas Res, 1(1), pp.24~ (2011)
- Ono H, "Hydrogen treatment for acute erythymatous skin diseases. A report of 4 patients with safety data and a non-controlled feasibility study with H2 concentration measurement on two volunteers", Med Gas Res, 2(1), pp.14~ (2012)
- Kang KM, "Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors", Med Gas Res, 1(1), pp.11~ (2011)
- Ishibashi T, "Consumption of water containing a high concentration of molecular hydrogen reduces oxidative stress and disease activity in patients with rheumatoid arthritis : an open-label pilot study", Med Gas Res, 2(1), pp.27~ (2012)