The Therapeutic Potential of Copper Peptides on Atopic Dermatitis Lesions in BALB/c mice and Human Keratinocytes
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
PURPOSE: Atopic dermatitis (AD), often referred to as eczema, is a chronic disease characterized by skin inflammation, itchiness, irritation, and dryness. It is caused mainly by immune dysregulation. Copper peptides (CP) have been used for a variety of skin-related purposes. In this study, we examined the effect of CP on AD induced in BALB/c mice. METHODS: BALB/c mice were separated into the following five groups: control, AD-only, AD+CP10, AD+CP100, and AD+CERA. AD was induced using house dust mite (HDM) extract and 2,4-dinitrochlorobenzene (DNCB). The mice received CP or ceramide (CERA; positive control) based on their group allocation. Histopathological analysis was performed to assess the effect of CP on mast cell infiltration. Dermal and epidermal thickness were measured using microphotographs of hematoxylin-and-eosin-stained tissue. We examined the serum immunoglobulin E (IgE) levels and mRNA gene expression of pathogenic cytokines in the mouse ear tissue and in human keratinocytes. RESULTS: CP treatment decreased dermal and epidermal mouse ear thickness and mast cell infiltration in the ear tissue in a dose-dependent manner. It also decreased the serum IgE levels and the mRNA gene expression of pathogenic cytokines in the ear tissue and in the human keratinocytes. Interestingly, the therapeutic effect of CP was more robust than that of CERA. CONCLUSIONS: These results indicate that CP may have therapeutic potential against AD.
Abstract
PURPOSE: Atopic dermatitis (AD), often referred to as eczema, is a chronic disease characterized by skin inflammation, itchiness, irritation, and dryness. It is caused mainly by immune dysregulation. Copper peptides (CP) have been used for a variety of skin-related purposes. In this study, we examined the effect of CP on AD induced in BALB/c mice. METHODS: BALB/c mice were separated into the following five groups: control, AD-only, AD+CP10, AD+CP100, and AD+CERA. AD was induced using house dust mite (HDM) extract and 2,4-dinitrochlorobenzene (DNCB). The mice received CP or ceramide (CERA; positive control) based on their group allocation. Histopathological analysis was performed to assess the effect of CP on mast cell infiltration. Dermal and epidermal thickness were measured using microphotographs of hematoxylin-and-eosin-stained tissue. We examined the serum immunoglobulin E (IgE) levels and mRNA gene expression of pathogenic cytokines in the mouse ear tissue and in human keratinocytes. RESULTS: CP treatment decreased dermal and epidermal mouse ear thickness and mast cell infiltration in the ear tissue in a dose-dependent manner. It also decreased the serum IgE levels and the mRNA gene expression of pathogenic cytokines in the ear tissue and in the human keratinocytes. Interestingly, the therapeutic effect of CP was more robust than that of CERA. CONCLUSIONS: These results indicate that CP may have therapeutic potential against AD.
참고문헌 (30)
- Bieber T, "Allergen-specific immunotherapy: current concepts and future directions: Editorial", Allergy, 66(6), pp.709~712 (2011)[DOI]
- Lee HS, "Licoricidin abrogates t-cell activation by modulating PTPN1 activity and attenuates atopic dermatitis in vivo", J Invest Dermatol, 141(10), pp.2490~2498 (2021)[DOI]
- Wollenberg A, "Atopic dermatitis in children and adults-diagnosis and treatment", Dtsch Arztebl Int, 120(13), pp.224~234 (2023)[DOI]
- Cooper KD, "Atopic dermatitis: recent trends in pathogenesis and therapy", J Invest Dermatol, 102(1), pp.128~137 (1994)[DOI]
- Bos JD, "Immune dysregulation in atopic eczema", Arch Dermatol, 128(11), pp.1509~1512 (1992)
- Jin H, "Animal models of atopic dermatitis", J Invest Dermatol, 129(1), pp.31~40 (2009)[DOI]
- Kabashima-Kubo R, "A group of atopic dermatitis without IgE elevation or barrier impairment shows a high Th1 frequency : possible immunological state of the intrinsic type", J Dermatol Sci, 67(1), pp.37~43 (2012)[DOI]
- Choi EJ, "Extracts of Grifola frondosa inhibit the MAPK signaling pathways involved in keratinocyte inflammation and ameliorate atopic dermatitis", Nutr Res Pract, 17(6), pp.1056~1069 (2023)[DOI]
- Grewe M, "A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis", Immunol Today, 19(8), pp.359~361 (1998)[DOI]
- Hamid Q, "Differential in situ cytokine gene expression in acute versus chronic atopic dermatitis", J Clin Invest, 94(2), pp.870~876 (1994)[DOI]
- Yamada N, "Chronologic analysis of in situ cytokine expression in mite allergen-induced dermatitis in atopic subjects", J Allergy Clin Immunol, 96(6), pp.1069~1075 (1995)[DOI]
- Vestergaard C, "Overproduction of Th2-specific chemokines in NC", Nga mice exhibiting atopic dermatitis-like lesions, 1999, pp.104(8), 1097~1105
- Rothenberg ME, "Common variants at 5q22 associate with pediatric eosinophilic esophagitis", Nat Genet, 42(4), pp.289~291 (2010)[DOI]
- He R, "Thymic stromal lymphopoietin", Ann N Y Acad Sci, 1183, pp.13~24 (2010)[DOI]
- Ziegler SF, "The biology of thymic stromal lymphopoietin(TSLP)", Adv Pharmacol, 66, pp.129~155 (2013)[DOI]
- Venge P, "Eosinophil and neutrophil granulocytes", Allergy, 48, pp.39~47 (1993)[DOI]
- Debnath T, "Natural products as a source of anti-inflammatory agents associated with inflammatory bowel disease", Molecules, 18(6), pp.7253~7270 (2013)
- Abdulghani AA, "Effects of topical creams containing vitamin C, a copper-binding peptide cream and melatonin compared with tretinoin on the ultrastructure of normal skin-a pilot clinical, histologic, and ultrastructural study", Disease Management and Clinical Outcomes, 4, pp.136~141 (1998)
- Appa Y, "A clinical evaluation of a copper-peptide containing liquid foundation and cream concealer designed for improving skin condition neutrogena corporation, los angeles, CA, stephens, T, TJ stephens & associates, Inc, Dallas, TX Abstract P66, American Academy of Dermatology Meeting", ~ (2002)
- Kim SH, "High-intensity swimming exercise increases dust mite extract and 1-chloro-2, 4-dinitrobenzene-derived atopic dermatitis in BALB", c mice, 2014, pp.37(4), 1179~1185
- Pickart L, "Copper peptides for aging reversal, body language dermatology", ~ (2003)
- Pickart L, "Copper peptides for tissue regeneration, specialty chemicals", 29~31 (2002)
- Dokmeci E, "The immune system and atopic dermatitis", Semin Cutan Med Surg, 27(2), pp.138~143 (2008)[DOI]
- Chen L, "Early up-regulation of Th2 cytokines and late surge of Th1 cytokines in an atopic dermatitis model", Clin Exp Immunol, 138(3), pp.375~387 (2004)[DOI]
- Neis MM, "Enhanced expression levels of IL-31 correlate with IL-4 and IL-13 in atopic and allergic contact dermatitis", J Allergy Clin Immunol, 118(4), pp.930~937 (2006)[DOI]
- Cianferoni A, "The importance of TSLP in allergic disease and its role as a potential therapeutic target", Expert Rev Clin Immunol, 10(11), pp.1463~1474 (2014)[DOI]
- Soumelis V, "Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP", Nat Immunol, 3(7), pp.673~680 (2002)[DOI]
- Lecron J, "Recent Advances in Skin Immunology" (2008), Kerala
- Boniface K, "IL-22inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes", J Immunol, 174(6), pp.3695~3702 (2005)[DOI]
- Bogiatzi SI, "Cutting Edge : proinflammatory and Th2 cytokines synergize to induce thymic stromal lymphopoietin production by human skin keratinocytes", J Immunol, 178(6), pp.3373~3377 (2007)[DOI]