ELISA-LC/MS/MS 병행에 의한 식품 중 aflatoxins 분석
Determination of Total Aflatoxins in Foods by Parallelism of ELISA and LC/MS/MS
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조회
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다운로드
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참고문헌
키워드
초록
High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin B1 (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2,walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid,accurate and cost-effective method for the detection of aflatoxins in large number of samples.
Abstract
High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin B1 (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2,walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid,accurate and cost-effective method for the detection of aflatoxins in large number of samples.
참고문헌 (32)
- Taber,H.H, "Aflatoxin producing potential of isolates of the Aspergillus flavusoryzae group from peanuts(Arachis hypogaea)", Journal of Applied Microbiology, 15, pp.140~144 (1967)
- Sweeney,M.J, "Mycotoxin production by Aspergillus, Fusarium and Penicillium species", International Journal of Food Microbiology, 43, pp.141~158 (1998)
- 김종규, "Occurrence of aflatoxins in rice and rice products and by-products of rice: A review", 한국환경보건학회지, 32(5), pp.515~521 (2006), 한국환경보건학회
- "Inte rnational Agency for Research on Cancer (IARC) :IARC monograph on the evaluation of carcinogenic risk to humans", pp.56~ (1993), IARC, Lyon
- Chiavaro, I, "Immunoaffinity clean-up and direct fluorescence measurement of aflatoxin B1 and M1 in pig liver: Comparison with high-performance liquid chromatography determination", Food Additives and Contaminants, 22(11), pp.1154~1161 (2005)
- 김종규, "유산균이 Aspergillus parasiticus의 성장과 Aflatoxin B1 생성에 미치는 영향", 한국환경보건학회지, 31(2), pp.127~133 (2005), 한국환경보건학회
- 강신정, "아플라톡신 오염 및 저감화 방안", 생약학회지, 38(3), pp.205~216 (2007), 한국생약학회
- Report on 1998 Korean national nutrition survey, , Ministry of Health & Welfare, ~ (2000)
- Shim, W. B, "Production of specific monoclonal antibody to aflatoxin B1 and its application to development of simultaneous immuno-detection techniques for mycotoxins", 박사, pp.2006
- Kim, J . K, "D etermination of aflatoxin u sing h igh performance liquid chromagraphy with optimized fluorescence detection", Journal of Food Hygiene and Safety, 13, pp.41~46 (1998)
- Schatzki,T.F, "Rapid, non-destructive selection of peanuts for high aflatoxin content by soaking and tandem ass spectrometry", Journal of Agricultural and Food Chemistry, 50, pp.3062~3069 (2002)
- Thean, J. E, "Extraction, clean up and quantitative determination of aflatoxin in corn", Journal of the Association Official Analytical Chemists, 63(3), pp.631~633 (1980)
- 황준호, "식품중의 Aflatoxins -분석방법 및 이화학적 반응을 통한 저감화를 중심으로-", Journal of the Korean Society for Applied Biological Chemistry, 47(1), pp.1~16 (2004), 한국응용생명화학회
- Papadopoulou-Bonuraoui,A, "Comparison of two post-column derivatization systems, ultraviolet irradiation and electrochemical determination, for the liquid chromatographic determination of aflatoxins in food", Journal of AOAC International, 85(2), pp.411~416 (2002)
- Kim, J. G, "Determination of aflatoxins using high-performance liquid chromatography and fluorescence or UV absorbence detection", Korean Journal of Environment Health Society, 22(1), pp.36~44 (1996)
- Nilufer,D, "Comparative study of three different methods for the determination of aflatoxins in tahini", Journal of Agricultural and Food Chemistry, 50, pp.3375~3379 (2002)
- Chun, H. S, "Determination of aflatoxin levels in nuts and their products consumed in South Korea", Food Chemistry, 120, pp.385~391 (2007)
- Kolosova, A. Y, "Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin B1. Stabilization of ELISA kit components and application to grain samples", Analytical and Bioanalytical Chemistry, 384, pp.286~294 (2006)
- Won-Bo Shim, "Determination of Aflatoxin B1 in Rice, Barley, and Feed by NoninstrumentalImmunochromatographic Strip-test and High SensitiveELISA", Food Science and Biotechnology, 17(3), pp.623~630 (2008), 한국식품과학회
- SHIM, WON-BO, "Development of Immunochromatography Strip-Test Using NanocolloidalGold-Antibody Probe for the Rapid Detection of Aflatoxin B1 in Grain andFeed Samples", Journal of Microbiology and Biotechnology, 17(10), pp.1629~1637 (2007), 한국미생물·생명공학회
- "AOAC international"[URL]
- IP,S.P, "Determination of aflatoxins in Chinese medicinal herbs by high-performance liquid chromatography using immunoaffinity column cleanup improvement of recovery", Journal of ChromatographyA, 1135, pp.241~244 (2006)
- Calleri, E, "Development and integration of an immunoaffinity monolithic disk for the on-line solidphase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solution", Journal of Pharmaceutical Biomedical Analysis, 44, pp.396~403 (2007)
- Giray, B, "Aflatoxin levels in wheat samples consumed in some regions of Turkey", Food Control, 18, pp.23~29 (2007)
- Chan, D, "Simultaneous determination of aflatoxins and ochratox in A in food u sing a fully automated immunoaffinity column clean-up and liquid chromatographyfluorescence detector", Journal of Chromatography A, 1059, pp.13~16 (2004)
- 이창희, "HPLC-FLD 및 LC-MS", MS에 의한 식품 중 총아플라톡신 오염실태 조사, 2007, pp.39(5), 488~493[DOI]
- Ok, K. S, "Advanced in the analysis of total aflatoxin in food", Journal of Food Hygiene and Safety, 21, pp.76~81 (2006)
- Aydin, A, "Determination of aflatoxin B1 levels in powdered red pepper", Food Control, 18, pp.1015~1018 (2007)
- Van Egmond, H. P, "Current situation on regulation for mycotoxins", pp.1~15 (2004), Japanese Association of Mycotoxicology
- 박민정, "식품 중 아플라톡신 오염도 조사", 한국식품위생안전성학회지, 23(2), pp.108~112 (2008), 한국식품위생안전성학회
- "Monitoring of mycotoxins in nuts" (2006), Korea Consumer Agency
- Choi, E. H, "Isolation of microorganisms from red pepper powder and their radiosensitivity", Korean Journal of Food Science and Technology, 9(3), pp.205~210 (1977)