돼지고기에서 7종 mycotoxins 잔류실태 조사
Monitoring of 7 mycotoxins in pork
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초록
This study was conducted to determine the content of 7 mycotoxins (aflatoxin B1, B2, G1, G2, M1, ochratoxin A and zearalenone) using LC-MS/MS in pork available on the Korean markets. The analysis was carried out using following conditions; C18 column (2.1×100 mm, 1.7 μm), mobile phase composed of H2O (0.1 mM NH4Ac 0.01% HCOOH)：Methanol (0.1 mM NH4Ac 0.01% HCOOH), binary pump at a flow rate of 0.5 mL/min and 2 μL of injection volume, MS/MS detector with ESI positive and negative mode. The quantication of mycotoxins was based on matrix-matched calibration curves with a correlation coefficient in excess of 0.99 for the 7 mycotoxins. The dectection limits were ranged 0.74∼2.13 ng/g, with mean recoveries between 73.10∼97.46% except aflatoxin B1 (61.31%). We also monitored mycotoxin residues in 208 pork samples. The test results, mycotoxins were not found except one sample. Ochratoxin A in one sample of the test samples was detected below the quantification limit.
Abstract
This study was conducted to determine the content of 7 mycotoxins (aflatoxin B1, B2, G1, G2, M1, ochratoxin A and zearalenone) using LC-MS/MS in pork available on the Korean markets. The analysis was carried out using following conditions; C18 column (2.1×100 mm, 1.7 μm), mobile phase composed of H2O (0.1 mM NH4Ac 0.01% HCOOH)：Methanol (0.1 mM NH4Ac 0.01% HCOOH), binary pump at a flow rate of 0.5 mL/min and 2 μL of injection volume, MS/MS detector with ESI positive and negative mode. The quantication of mycotoxins was based on matrix-matched calibration curves with a correlation coefficient in excess of 0.99 for the 7 mycotoxins. The dectection limits were ranged 0.74∼2.13 ng/g, with mean recoveries between 73.10∼97.46% except aflatoxin B1 (61.31%). We also monitored mycotoxin residues in 208 pork samples. The test results, mycotoxins were not found except one sample. Ochratoxin A in one sample of the test samples was detected below the quantification limit.
참고문헌 (19)
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