Studies on mycotoxins using LC-MS/MS for the forage produced in Incheon
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The purpose of this study was to investigate the contamination level of representative mycotoxins that have adverse effects on livestock by using LC-MS/MS method and to utilize the results as basic data for the establishment of quality control system for feed, and to provide information on production and storage. A total of nine mycotoxins, including aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, och-ratoxin A, fumonisin B1, fumonisin B2, deoxynivalenol (DON), zearalenone (ZEN) were simultaneously analyzed in LC-MS/MS under ESI positive mode. Fumonisin B1 and fumonisin B2 were detected from 3 cases of 75 forage produced in Incheon area, the detection rate was 4.0%. The detection concentra-tion was 0.01∼0.02 mg/kg, which was lower than the domestic recommended limit. Fumonisins were detected in a slightly different manner from the results of mycotoxin studies reported in Korea, which is attributed to the high temperature and dry summer weather of the year. The result of LC-MS/MS method performance of 9 mycotoxins, the recovery of DON was quite low as 41.53±3.91% that is not suitable for simultaneous analysis. This is probably due to that the extract solution used in this study was not suitable for the extraction of DON, along with the characteristics of a very dry forage. For the study of mycotoxins in Incheon area forage for the first time, further investigation is needed for the safe supply of livestock products.
Abstract
The purpose of this study was to investigate the contamination level of representative mycotoxins that have adverse effects on livestock by using LC-MS/MS method and to utilize the results as basic data for the establishment of quality control system for feed, and to provide information on production and storage. A total of nine mycotoxins, including aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, och-ratoxin A, fumonisin B1, fumonisin B2, deoxynivalenol (DON), zearalenone (ZEN) were simultaneously analyzed in LC-MS/MS under ESI positive mode. Fumonisin B1 and fumonisin B2 were detected from 3 cases of 75 forage produced in Incheon area, the detection rate was 4.0%. The detection concentra-tion was 0.01∼0.02 mg/kg, which was lower than the domestic recommended limit. Fumonisins were detected in a slightly different manner from the results of mycotoxin studies reported in Korea, which is attributed to the high temperature and dry summer weather of the year. The result of LC-MS/MS method performance of 9 mycotoxins, the recovery of DON was quite low as 41.53±3.91% that is not suitable for simultaneous analysis. This is probably due to that the extract solution used in this study was not suitable for the extraction of DON, along with the characteristics of a very dry forage. For the study of mycotoxins in Incheon area forage for the first time, further investigation is needed for the safe supply of livestock products.
참고문헌 (21)
- Auerbach H., "Nutritional biotechnology in the feed and food industries", pp.247~265 (2003), Nottingham Univ. Press
- "Mycotoxin survey 2016 third quarter" (2016)[URL]
- Mycotoxins : economics and health risks, "CAST(Council for Agricultural Science & Technology)", ~, pp.Task Force Report No.116 (1989)
- Mycotoxins : lisks in plant, animal and human systems, "CAST(Council for Agricultural Science & Technology)", ~, pp.Task Force Report No. 139 (2003)
- D'Mello JPF, "Fusarium mycotoxins : a review of global implications for animal health, welfare and productivity", Anim Feed Sci Tech, 80, pp.183~205 (1999)
- Fink-Gremmels J., "The mycotoxin blue book", pp.249~268 (2005), Nottingham Univ. Press
- Kim CJ, "Food microbiology" (1999), Soohaksa
- Forage supply policy direction, "Ministry of Agriculture, Food and Rural Affairs", 4~23 (2014)
- Ministry of Agriculture, Food and Rural Affairs, "Control of livestock and fish feed act(notofication No. 14481)", ~ (2016)
- Ministry of Agriculture, Food and Rural Affairs, "Acceptance criteria from Standards for feed(notofication No. 2017-28)", ~ (2017)
- National institute of agricultural sciences, Rural development administration, "Understanding and prevention of mycotoxins", ~ (2016)
- The method to analyze mycotoxins in food in 2016, "National institute of food and drug safety evaluation", 61~63 (2016)
- Nelson CE, "Strategies of mold control in dairy feeds", J Dairy Sci, 76, pp.898~902 (1993)
- Nho WG, "Taxonomy and Identification of fungi isolated from round bale silage", Journal of Practical Agriculture and Fisheries Research, 14, pp.61~80 (2012)
- Santi Devi Upadhaya, "Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats", Journal of Veterinary Science, 10(1), pp.29~34 (2009), 대한수의학회
- Schatzmayr G., "Effective solution to control mycotoxins", pp.W42~W43 (2005), Hoard’s West
- Scudamore KA, "Occurrence and significance of mycotoxins in forage crops and silage; a review", J Sci Food Agric, 77, pp.1~17 (1998)
- 성하균, "국내 조사료용 볏짚의 수확 전 작물포 및 수확 후 저장에 따른 곰팡이독소 오염 실태 연구", 한국초지조사료학회지, 33(1), pp.21~29 (2013), 한국초지조사료학회
- Sung HG, "The presence of mycotoxigenic fungi in rice straw during the earlier spring of Korea. New paradigm for forage production in the East Asian region", Proceeding of the 3rd Korea-China-Japan joint symposium on Grassland Agriculture and Livestock Production, pp.48~49 (2009)
- 성하균, "사료용 볏짚 곤포사일리지의 곰팡이 및 Mycotoxin 오염 연구", 한국초지조사료학회지, 31(4), pp.451~462 (2011), 한국초지조사료학회[DOI]
- 성하균, "조사료의 곰팡이 발생과 곰팡이독소 오염", 한국초지조사료학회지, 30(1), pp.77~88 (2010), 한국초지조사료학회