주거환경 공기 중 호흡기 바이러스의 검출
Detection of Airborne Respiratory Viruses in Residential Environments
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Objectives: Respiratory virus infections are the most common disease among all ages in all parts of the world and occur through airborne transmission. The purpose of this study was to detect and quantitate human respiratory viruses in residential environments. Methods: Air samples were collected from the residential space of apartments in the Seoul/Gyeonggi-do area. The samples were collected from indoor and outdoor air. Among respiratory viruses, influenza A virus, influenza B virus, parainfluenza virus, metapneumovirus, respiratory syncytial virus, and adenovirus were investigated by multiplex polymerase chain reaction. Among the virus-positive samples, we performed adenovirus quantification by real-time polymerase chain reaction. Results: Virus detection rates were 44.0%, 3.8%, 3.4%, and 17.3% in spring, summer, autumn, and winter, respectively. The virus detection rate was higher in winter and spring than in summer and autumn. Adenovirus was most commonly detected, followed by influenza A virus and parainfluenza virus. Virus distribution was not significantly different between indoor and outdoor environments. Conclusions: Although virus concentrations were not high in residential environments, residents in houses with detected viruses may have an increased risk of exposure to airborne respiratory viruses, especially in winter and spring.
Abstract
Objectives: Respiratory virus infections are the most common disease among all ages in all parts of the world and occur through airborne transmission. The purpose of this study was to detect and quantitate human respiratory viruses in residential environments. Methods: Air samples were collected from the residential space of apartments in the Seoul/Gyeonggi-do area. The samples were collected from indoor and outdoor air. Among respiratory viruses, influenza A virus, influenza B virus, parainfluenza virus, metapneumovirus, respiratory syncytial virus, and adenovirus were investigated by multiplex polymerase chain reaction. Among the virus-positive samples, we performed adenovirus quantification by real-time polymerase chain reaction. Results: Virus detection rates were 44.0%, 3.8%, 3.4%, and 17.3% in spring, summer, autumn, and winter, respectively. The virus detection rate was higher in winter and spring than in summer and autumn. Adenovirus was most commonly detected, followed by influenza A virus and parainfluenza virus. Virus distribution was not significantly different between indoor and outdoor environments. Conclusions: Although virus concentrations were not high in residential environments, residents in houses with detected viruses may have an increased risk of exposure to airborne respiratory viruses, especially in winter and spring.
참고문헌 (26)
- van den Hoogen BG, "Clinical impact and diagnosis of human metapneumovirus infection", Pedistr Infect Dis J, 23(1), pp.S25~S32 (2004)
- Denny Jr, FW, "The clinical impact of human respiratory virus infections", Am J Respir Care Med, 152(4), pp.4~12 (1995)
- Erard V, "Quantitative real-time polymerase chain reaction for detection of adenovirus after T cell-replete hematopoietic cell transplantation: viral load as a marker for invasive disease", Clin Infect Dis, 45(8), pp.958~965 (2007)
- Hierholzer JC, "Adenovirus in the immunocompromised host", Clin Microbiol Rev, 5(3), pp.262~274 (1992)
- Rivers MN, "Hantavirus pulmonary syndrome in Texas: 1993- 2006", South Med J, 102(1), pp.36~41 (2009)
- Lee JY, "Between life and death of the virus, 1st ed", pp.278~ (2005), Jiho publishing
- Isaacs D, "Handbook of Neonatal Infections, 1st ed", pp.53~78 (1999), W B Saunders company
- Moser MR, "An outbreak of influenza abroad a commercial airliner", Am J Epidemiol, 110(1), pp.1~6 (1979)
- Kenyon TA, "Transmission of multidrug-resistant Mycobacterium tuberculosis during a long airplane flight", N Engl J Med, 334(15), pp.933~938 (1996)
- Myatt TA, "Detection of airborne rhinovirus and its relation to outdoor air supply in office environments", Am J Respir Crit Care Med, 169(11), pp.1187~1190 (2004)
- 김선형, "2004-2006년의 호흡기 바이러스 감염의 역학", Annals of Laboratory Medicine, 26(5), pp.351~357 (2006), 대한진단검사의학회
- "Public health weekly", 4(15), pp.269~ (2011), Korea Centers for Disease Control
- 강정옥, "국내 의료기관의 호흡기바이러스 검사현황 및 5개 대학병원에서검출된 호흡기바이러스 역학", 대한임상미생물학회지, 10(2), pp.102~108 (2007), 대한임상미생물학회
- Ahn KM, "Clinical characteristics of acute viral lower respiratory tract infections in hospitalized children in seoul, 1996-1998", J Korean Med Sci, 14(4), pp.405~411 (1999)
- 정희영, "다중 역전사 중합연쇄반응으로 증명된 급성 바이러스성 하기도 감염 양상", 대한소아알레르기및호흡기학회지, 17(4), pp.344~353 (2007), 대한 소아알레르기 호흡기학회
- Echavarria MM, "PCR method for detection of adenovirus in urine of healthy and human immunodeficiency virus infected individuals", J Clin Microbiol, 36(11), pp.3323~3326 (1998)
- Schilham MW, "High levels of adenovirus DNA in serum correlate with fatal outcome of adenovirus infection in children after allogeneic stem-cell transplantation", Clin Infect Dis, 35(5), pp.526~532 (2002)
- Harper GJ, "Airborne microorganism: Survival tests with four viruses", J Hyg, 59, pp.479~486 (1961)
- Miller WS, "Aerosol stability of three acute respiratory disease viruses", Proc Soc Exp Biol Med, 125(1), pp.222~227 (1967)
- Mahl MC, "Virus survival on inanimate surfaces", Can J Microbiol, 21(6), pp.819~823 (1975)
- Benbough JE, "The effect of relative humidity on the survival of airborne SemliKi Forest virus", J Gen Virol, 4(4), pp.473~477 (1969)
- Webb SJ, "The effect of relative humidity and inositol on airborne viruses", Can J Microbiol, 9, pp.87~92 (1963)
- Elazhary MA, "Aerosol stability of bovine adenovirus type 3", Can J Comp Med, 43(3), pp.305~312 (1979)
- Davis GW, "Effect of relative humidity on dynamic aerosols of adenovirus 12", Appl Microbiol, 21(4), pp.676~679 (1971)
- Lowen AC, "Influenza virus transmission is dependent on relative humidity and temperature", PLoS Pathog, 3(10), pp.1470~1476 (2007)
- Freymuth F, "Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness", J Med Virol, 78, pp.1498~1504 (2006)