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調經種玉湯이 Estradiol Valerate로 유발된 다낭성 난소 모델에서 난포의 성숙 및 NGF 발현에 미치는 影響

Effects of Jokyeongjongok-Tang on the Maturation of Follicles and NGF Expression in Rats with Estradiol Valerate-induced Polycystic Ovaries

김형준|이동녕|서일복|양준모
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2011.05|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제24권 제2호|pp. 52-67

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참고문헌

키워드

Jokyeongjongok-TangPolycystic ovariesEstradiol valerateOvarian histopathologyNerve growth factorCorticotropin releasing factor
주제분류한의학

초록

Objectives: This study was designed to investigate the effects of Jokyeongjongok -Tang(JJT) on the progression of the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4 mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. JJT treated group(n=8) were injected with EV and orally administrated JJT for same duration. At the end day of experiment, we measured weights of body, ovaries, adrenal glands and uterus. The histopathological changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(㎎) of ovaries of JJT treated group(58.4±9.4) were significantly increased(p<0.01) compared with PCO control group(42.3±8.5). - The numbers of mature follicles of JJT treated group(10.1±2.5) were significantly increased(p<0.01) compared with PCO control group(6.1±2.1). - The numbers of cystic follicles of JJT treated group(1.8±1.4) were significantly decreased(p<0.05) compared with PCO control group(3.8±1.5). - The expressions of NGF-immunoreactive cells in the ovaries of JJT treated group were weaker than PCO control group. Conclusions: From the above results, we concluded that Jokyeongjongok-Tang (JJT) contributes to a normal maturation of follicles and has the effects of promoting a normal ovulation. And these effects may be related with the decreased NGF activities in the ovaries.

Abstract

Objectives: This study was designed to investigate the effects of Jokyeongjongok -Tang(JJT) on the progression of the estradiol valerate(EV)-induced polycystic ovaries(PCO) in rats. Methods: PCO was induced by single intramuscular injection with estradiol valerate(EV)(4 mg) in female rats. Normal group(n=8) were injected with sesame oil and orally administrated distilled water for sixty days. PCO control group (n=8) were injected with EV and orally administrated distilled water for sixty days. JJT treated group(n=8) were injected with EV and orally administrated JJT for same duration. At the end day of experiment, we measured weights of body, ovaries, adrenal glands and uterus. The histopathological changes of ovaries were also evaluated. And we observed the NGF and CRF expression by immunohistochemistry. Results: The results were as follows - The weights(㎎) of ovaries of JJT treated group(58.4±9.4) were significantly increased(p<0.01) compared with PCO control group(42.3±8.5). - The numbers of mature follicles of JJT treated group(10.1±2.5) were significantly increased(p<0.01) compared with PCO control group(6.1±2.1). - The numbers of cystic follicles of JJT treated group(1.8±1.4) were significantly decreased(p<0.05) compared with PCO control group(3.8±1.5). - The expressions of NGF-immunoreactive cells in the ovaries of JJT treated group were weaker than PCO control group. Conclusions: From the above results, we concluded that Jokyeongjongok-Tang (JJT) contributes to a normal maturation of follicles and has the effects of promoting a normal ovulation. And these effects may be related with the decreased NGF activities in the ovaries.

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