龍膽瀉肝湯이 Estradiol Valerate로 유발된 흰쥐의 다낭성 난소 발달과 수태에 미치는 영향
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Objectives: This study was designed to investigate the effects of Yongdamsagan- tang on the polycystic ovary(PCO) induced by estradiol valerate(EV) in rats. Methods: After administrating Yongdamsagan-tang to PCO induced rats, we measured the weight of body, ovaries, adrenal glands, and uterus of rats. The observation through naked eye and histopathological observation of ovaries were evaluated. Also, the number of follicle and corpora lutea and content of androstenedione(ADD) and total estrogen were evaluated. The expressions of nerve growth factor(NGF) and corticotropin releasing factor(CRF) were analyzed by immunohistochemistry. The breeding rate and number of implantation with normal male rats were evaluated. Results:- The weight(㎎) of ovaries in YST treated group(73.8±7.6) was significantly increased(p<0.001) compared with control group(54.3±4.5). - The number of mature follicles in YST treated group(7.3±2.4) was significantly increased(p<0.01) compared with control group(3.5±1.2). - The number of atretic follicles in YST treated group(9.0±1.5) was significantly decreased(p<0.01) compared with control group(13.4±3.8). - The number of cystic follicles in YST treated group(3.1±1.1) was significantly decreased(p<0.01) compared with control group(6.0±2.0). - The number of corpora lutea in YST treated group(3.8±2.1) was significantly increased(p<0.001) compared with control group(0.3±0.7). - The expression of NGF-immunoreactive cells in the ovarian granulosa cells in YST treated group was lesser observed than control group. - The expression of NGF-immunoreactive cells in the adrenal cortex in YST treated group was lesser observed than control group. - The breeding rate in YST treated group(100 %) was significantly increased (p<0.05) compared with control group(50 %). - The number of implantation in YST treated group(6.4±4.7) was significantly increased(p<0.05) compared with control group(1.4±2.6). Conclusions: We concluded that Yongdamsagan-tang activates the maturation of follicles, normal ovulation, breeding rate and number of implantation. Therefore, this may be effective for the treatment of anovulation, amenorrhea and sterility of PCOS patients.
Abstract
Objectives: This study was designed to investigate the effects of Yongdamsagan- tang on the polycystic ovary(PCO) induced by estradiol valerate(EV) in rats. Methods: After administrating Yongdamsagan-tang to PCO induced rats, we measured the weight of body, ovaries, adrenal glands, and uterus of rats. The observation through naked eye and histopathological observation of ovaries were evaluated. Also, the number of follicle and corpora lutea and content of androstenedione(ADD) and total estrogen were evaluated. The expressions of nerve growth factor(NGF) and corticotropin releasing factor(CRF) were analyzed by immunohistochemistry. The breeding rate and number of implantation with normal male rats were evaluated. Results:- The weight(㎎) of ovaries in YST treated group(73.8±7.6) was significantly increased(p<0.001) compared with control group(54.3±4.5). - The number of mature follicles in YST treated group(7.3±2.4) was significantly increased(p<0.01) compared with control group(3.5±1.2). - The number of atretic follicles in YST treated group(9.0±1.5) was significantly decreased(p<0.01) compared with control group(13.4±3.8). - The number of cystic follicles in YST treated group(3.1±1.1) was significantly decreased(p<0.01) compared with control group(6.0±2.0). - The number of corpora lutea in YST treated group(3.8±2.1) was significantly increased(p<0.001) compared with control group(0.3±0.7). - The expression of NGF-immunoreactive cells in the ovarian granulosa cells in YST treated group was lesser observed than control group. - The expression of NGF-immunoreactive cells in the adrenal cortex in YST treated group was lesser observed than control group. - The breeding rate in YST treated group(100 %) was significantly increased (p<0.05) compared with control group(50 %). - The number of implantation in YST treated group(6.4±4.7) was significantly increased(p<0.05) compared with control group(1.4±2.6). Conclusions: We concluded that Yongdamsagan-tang activates the maturation of follicles, normal ovulation, breeding rate and number of implantation. Therefore, this may be effective for the treatment of anovulation, amenorrhea and sterility of PCOS patients.
참고문헌 (60)
- Roger P. Smith, "Netter's Obstetrics & Gynecology", pp.371~372 (2005), Women's Health
- 김탁, "다낭성 난소 증후군의 최신지견", 대한산부인과학회. 연수강좌, 31, pp.38~44 (2002)
- "The Rotterdam ESHRE", ASRM-sponsored PCOS consensus workshop group: Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome, 2004, pp.81, 19~25
- 노정현, "다낭성 난소 증후군의 진단과 치료", 대한내분비학회. 연수강, 58~66 (2007)
- 최영길, "내분비학", pp.563~571 (1994), 의학출판사
- Abbott DH, "Developmental origin of polycystic ovary syndrome - a hypothesis", J Endocrinol, 1741, pp.1~5 (2002)
- 陳貴廷, "實用中西醫結合診斷治療學", pp.1004~1009 (1991), 中國醫學科技出版社
- 張鳳嬋, "中醫藥治療多囊卵巢綜合症方法初探", 新中醫, 1, pp.14~16 (1991)
- 施令義, "中西醫結合硏究學術論文編", 婦産科專緝, 10, pp.32~ (1983)
- 吳桂芳, "中醫藥治療多囊卵巢綜合症硏究槪況", 河南中醫, 14(2), pp.123~125 (1994)
- 金維新, "不孕症的診斷與中醫治療", pp.172~177 (1992), 北京科學出版社
- 周羽珍, "蔡小蓀治療婦科痼疾驗方拾萃", 江蘇中醫, 17(1), pp.9~10 (1996)
- 양승정, "蒼附導痰湯이 estradiol valerate로 유발된 백서의 다낭성 난소에 미치는 影響", 대한한방부인과학회지, 15(2), pp.1~11 (2002), 대한한방부인과학회
- 유영기, "腎氣丸合蒼附導痰湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소에 미치는 영향", 대한한방부인과학회지, 20(1), pp.77~92 (2007), 대한한방부인과학회
- 남은정, "定經湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소에 미치는 영향", 대한한방부인과학회지, 20(1), pp.93~116 (2007), 대한한방부인과학회
- 이연경, "歸脾湯이 Estradiol Valerate로 유도된 흰쥐의 다낭성 난소에 미치는 영향", 대한한방부인과학회지, 21(3), pp.60~74 (2008), 대한한방부인과학회
- "Encyclopaedia Britannica, 제15판"
- Brawer JR, "Development of the polycystic ovarian condition(PCO) in the estradiol valerate -treated rat", Biol Reprod, 35, pp.647~655 (1986)
- 민응기, "다낭성 난소 증후군", Obstetrics & Gynecology Science, 51(8), pp.805~819 (2008), 대한산부인과학회
- Stein IF, "Amenorrhea associated with bilateral polycystic ovaries", Am J Obstet Gynecol, 29, pp.181~191 (1935)
- Barbieri RL, "The role of hyperinsulinemia in the pathogenesis of ovarian hyperandrogenism", Fertil Steril, 50, pp.197~212 (1988)
- Frank S, "Polycystic ovary syndrome: a changing perspective", Clin Endocrinol, 31, pp.87~120 (1989)
- Vink JM, "Heritability of polycystic ovary syndrome in a Dutch tein-family study", J Clin Endocrinol Metab, 91, pp.2100~2104 (2006)
- 許俊, "東醫寶鑑", pp.823~824 (2005), 東醫寶鑑出版社
- 신웅호, "착상과 내분비계", 대한산부인과학회지, 23(1), pp.11~18 (1980)
- Nestler JE, "Insulin stimulates testosterone biosynthesis by human theca cells from women with polycystic ovary syndrome by activating its own receptor and using inositolglycan mediators as the signal transduction system", J Clin Endocrinol Metab, 83, pp.2001~2005 (1998)
- Barbieri RL, "The role of hyperinsulinemia in the pathogenesis of ovarian hyperandrogenism", Fertil Steril, 50, pp.197~212 (1988)
- Kalme T, "Comparative studies on the regulation of insulin-like growth factor-binding protain-1(IGFBP-1) and sex hormone-binding globulin (SHBG) production by insulin and insulin-like growth factors in human hepatoma cell", J Steroid Biochem Mol Biol, 86, pp.197~200 (2003)
- Waldstreicher J, "Hyperfunction of the hypothalamic-pituitary axis in women with polycystic ovarian disease: indirect evidence for partial gonadotroph desensitization", J Clin Endocrinol Metab, 66, pp.165~172 (1988)
- Stener-Victorin E, "Effects of electro-acupuncture on nerve growth factor and ovarian morphology in rats with experimentally induced polycystic ovaries", Biol Reprod, 63(5), pp.1497~1503 (2000)
- 김인주, "다낭성 난소 증후군과 여성 건강", 대한내분비학회지, 10(3), pp.187~203 (2001)
- Barbieri RL, "Hyperandrogenism, insulin resistance, and acanthosis nigricans syndrome: A common endocrinopathy with distinct pathophysiologic features", Am J Obstet Gynecol, 147(1), pp.90~101 (1983)
- 이영호, "Medroxy Progesterone Acetate(MPA)가 생쥐의 교미 및 배란에 미치는 영향", 대한산부인과학회지, 20(8), pp.553~564 (1977)
- Young, W.C, "Changes in selected features of behavior in pairs of opposely sexed chimpanzees during the sexual cycle and after ovariectomy", J. Comp. Psychol, 37, pp.107~ (1994)
- 윤소영, "다낭성 난소 증후군 환자의 한방 치료에 대한 임상적 고찰", 대한한방부인과학회지, 13(2), pp.437~451 (2000)
- 김동일, "다낭성 난소 증후군에 관한 문헌적 고찰", 대한한방부인과학회지, 10(1), pp.73~91 (1997)
- Azziz R, "Positions statement: criteria for defining polycystic ovary syndrome as a predominantly hyperandrogenic syndrome: an Androgen Excess Society guideline", J Clin Endocrinol Metab, 91, pp.4237~4245 (2006)
- 강길전, "여성생식의학", pp.219~226 (1998), 대관출판사
- Carmina E, "Does ethnicity influence the prevalence of adrenal hyperandrogenism and insulin resistance in polycystic ovary syndrome", Am J Obstet Gynecol, 167, pp.1807~1812 (1992)
- Goldzieher JW, "The polycystic ovary. Ⅶ. Intractable uterine bleeding and endometrial hyperplasia possibly related to an extraovarian source of oestrogen", Acta Endocrinol (Copenh), 54, pp.452~466 (1967)
- Balen AH, "Ultrasound assessment of the polycystic ovary: international consensus definitions", Hum Reprod Update, 9, pp.505~514 (2003)
- 성연아, "다낭성 난소 증후군의 내과적 진단과 치료", 대한내과학회지, 70, pp.356~360 (2006)
- Armanini D, "In-vivo metabolites of spironolactone and potassium canrenoate: determination of potential anti-androgenic activity by a mouse kidney cytosol receptor assay", Clin Endocrinol(Oxf), 23, pp.341~347 (1985)
- Kousta E, "Modern use of climiphene citrate in induction of ovulation", Hum Reprod Update, 3(4), pp.359~365 (1997)
- Hamilton-Fairley D, "Low-dose gonadotropin therapy for induction of ovulation in 100 women with polycystic ovary syndrome", Hum Reprod, 6, pp.1095~1099 (1991)
- Biljan MM, "The outcome of 150 babies following the treatment with letrozole or letrozole and gonadotropins", Fertil Steril, 84, pp.95~ (2005)
- 李東垣, "東垣十種醫書", pp.219~220 (1983), 大星文化社
- 羅元榿, "實用中醫婦科學", pp.271~275 (1994), 上海科學技術出版社
- Lara HE, "Activation of ovarian sympathetic nerves in polycystic ovary syndrome", Endocrinology, 133(6), pp.2690~2695 (1993)
- Nevi-Montalcini R, "The nerve growth factor 35 years later", Science, 237, pp.1154~1162 (1987)
- Lara HE, "Involvement of nerve growth factor in female sexual development", Endocrinology, 126(1), pp.364~375 (1990)
- Dissen GA, "A role for trkA nerve growth factor receptors in mammalian ovulation", Endocrinology, 137(1), pp.198~209 (1996)
- Dissen GA, "Intraovarian excess of nerve growth factor increase androgen secretion and disrupts estrous cyclicity in the rat. Endocrinology", Endocrinology, 141(3), pp.1073~1081 (2000)
- Dyer CA, "Norepinephrine amplifies human chorionic gonadotropin -stimulated androgen biosynthesis by ovarian theca-interstitial cells", Endocrinology, 116(4), pp.1645~1652 (1985)
- Barria A, "Ovarian steroidal response to gonadotropins and beta -adrenergic stimulation is enhanced in polycystic ovarian syndrome:role of the sympathetic innervation", Endocrinology, 133(6), pp.2696~2703 (1993)
- Hisano S, "Reciprocal synaptic relations between CRF-immunoreactive and TRH-immunoreactive neurons in the paraventricular nucleus of the rat hypothalamus", Brain Res, 620, pp.343~346 (1993)
- Lenz HJ, "Central nervous system effects of corticotropin-releasing factor on gastrointestinal transit in the rat", Gastroenterology, 94, pp.598~602 (1988)
- Stener-Victorin E, "Steroid- induced polycystic ovaries in rats: effect of electro-acupuncture on concentrations of endothelin-1 and nerve growth factor (NGF), and expression of NGF mRNA in the ovaries, the adrenal glands, and the central nervous system", Reprod Biol Endocrinol, 1, pp.33~ (2003)
- Goldzieher JW, "The polycystic ovary. clinical and histological features", J Clin Endoc Metab, 22, pp.325~ (1963)
- 이성수, "수정란 착상에 영향을 미치는 자궁내인자에 관한 연구", 대한수의학회지, 32(4), pp.531~542 (1992)
