虎杖根의 파골세포 분화 억제 효과와 기전 연구
Osteoclast Differentiation of Polygoni Cuspidati Radix Extracts Effects and Mechanism of Inhibition Studies
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Objectives: This study was conducted to evaluate the inhibitory effect of polygonicuspidati radix (PCR) extract on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of PCR extractin BMMs stimulated with RANKL. Tartrate resistant acid phosphatase (TRAP)staining, TRAP activity and RT-PCR were performed to know the inhibitory effecton osteoclast differentiation. actin ring formation were analysed to observe theeffect of PCR extract. Results: PCR decreased the number of TRAP positive cells and TRAP activitiesin BMMs stimulated with RANKL and M-CSF. PCR restrained the formation ofactin ring. PCR down regulated the induction of NFATc1, c-Fos, TRAP and OSCARby RANKL. PCR inhibited NF-κB activity by inducing degradation of IκBα. Conclusions: We suggest that PCR Extracts can be an effective therapeutic agenton osteoclast differentiation caused by diseases such as osteoporosis.
Abstract
Objectives: This study was conducted to evaluate the inhibitory effect of polygonicuspidati radix (PCR) extract on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of PCR extractin BMMs stimulated with RANKL. Tartrate resistant acid phosphatase (TRAP)staining, TRAP activity and RT-PCR were performed to know the inhibitory effecton osteoclast differentiation. actin ring formation were analysed to observe theeffect of PCR extract. Results: PCR decreased the number of TRAP positive cells and TRAP activitiesin BMMs stimulated with RANKL and M-CSF. PCR restrained the formation ofactin ring. PCR down regulated the induction of NFATc1, c-Fos, TRAP and OSCARby RANKL. PCR inhibited NF-κB activity by inducing degradation of IκBα. Conclusions: We suggest that PCR Extracts can be an effective therapeutic agenton osteoclast differentiation caused by diseases such as osteoporosis.
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