본문 바로가기
KERD
논문KCI 등재

ST25(天樞)에 대한 황련해독탕 약침이 DSS로 유발된 대장염 백서 모델에 미치는 영향

The Effect of Hwangnyeonhaedok-tang Pharmacopuncture on ST25 (天樞) in Rats with Dextran Sulfate Sodium (DSS)-Induced Colitis

조성희|양승정|윤대환|박경미|이승헌
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2016.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제29권 제1호|pp. 1-13|DOI http://dx.doi.org/10.15204/jkobgy.2016.29.1.001

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

colitisdextran sulfate sodium (DSS)ST25Hwangnyeonhaedok-tang pharmacopuncture (HTTP)
주제분류한의학

초록

Objectives: The purpose of this study is to find out the effect of various concentrations of Hwangnyeonhaedok-tang (HTT) pharmacopuncture on ST25 (天樞) in rats with dextran sulfate sodium (DSS)-induced colitis. Methods: Colitis was experimentally induced by feeding rats with water mixed with 5% (w/v) DSS for 20 days. The rats were divided into 5 groups: the normal group (Nor, n=5), the control group - colitis induced rats with no treatment (Con, n=5), the acupuncture group - colitis induced rats with acupuncture applied on ST25 (Acu, n=5), the pharmacopuncture group 1 - colitis induced rats with 0.729 mg/250 g/40 μl of pharmacopuncture applied on ST25 (PA-1, n=5), the pharmacopuncture group 2 - colitis induced rats with 3.645 mg/250 g/40 μl of pharmacopuncture applied on ST25 (PA-2, n=5). The changes in weight, excrement concentration and hemafecia were observed 5 times every 2 days. The colon lengths were measured from appendix to the end of colon after the experiment. Hematological and serological exams were conducted the day after the last treatment by cardiac puncturing anesthetized rats. Results: ST25 is the abdominal front point (募穴) of large intestine meridian and is known to have effect in colitis. Various concentrations of HTT pharmacopuncture (HTTP) applied on ST25, in rats with DSS-induced colitis inhibited decrease in colon lengths and body weight in both PA-1 and PA-2 groups. Hematological and serological exam results also showed that HTTP has significant effect on colitis in both PA-1 and PA-2 groups. Conclusions: Colon lengths were significantly increased in the acupuncture group, PA-1 group and PA-2 group, compared to the control group. The body weight was significantly increased (p<0.05) in PA-2 group after the first treatment, compared to the control group. TNF-𝛼, IL-6, AST were significantly decreased in PA-1 and PA-2 groups, compared to the control group.

Abstract

Objectives: The purpose of this study is to find out the effect of various concentrations of Hwangnyeonhaedok-tang (HTT) pharmacopuncture on ST25 (天樞) in rats with dextran sulfate sodium (DSS)-induced colitis. Methods: Colitis was experimentally induced by feeding rats with water mixed with 5% (w/v) DSS for 20 days. The rats were divided into 5 groups: the normal group (Nor, n=5), the control group - colitis induced rats with no treatment (Con, n=5), the acupuncture group - colitis induced rats with acupuncture applied on ST25 (Acu, n=5), the pharmacopuncture group 1 - colitis induced rats with 0.729 mg/250 g/40 μl of pharmacopuncture applied on ST25 (PA-1, n=5), the pharmacopuncture group 2 - colitis induced rats with 3.645 mg/250 g/40 μl of pharmacopuncture applied on ST25 (PA-2, n=5). The changes in weight, excrement concentration and hemafecia were observed 5 times every 2 days. The colon lengths were measured from appendix to the end of colon after the experiment. Hematological and serological exams were conducted the day after the last treatment by cardiac puncturing anesthetized rats. Results: ST25 is the abdominal front point (募穴) of large intestine meridian and is known to have effect in colitis. Various concentrations of HTT pharmacopuncture (HTTP) applied on ST25, in rats with DSS-induced colitis inhibited decrease in colon lengths and body weight in both PA-1 and PA-2 groups. Hematological and serological exam results also showed that HTTP has significant effect on colitis in both PA-1 and PA-2 groups. Conclusions: Colon lengths were significantly increased in the acupuncture group, PA-1 group and PA-2 group, compared to the control group. The body weight was significantly increased (p<0.05) in PA-2 group after the first treatment, compared to the control group. TNF-𝛼, IL-6, AST were significantly decreased in PA-1 and PA-2 groups, compared to the control group.

참고문헌 (36)

  1. Braunwald E, "Harrison’s principles of internal medicine", pp.2273~2277 (2010), MIP
  2. Kirsner JB, "Recent developments in ‘non specific' inflammatory bowel disease", N Engl Med, 306, pp.306~775 (1982)
  3. Bayless TM, "Current management of inflammatory bowel disease", Journal of Clinical Gastroenterology, 12(5), pp.609~ (1989)
  4. Geller AJ, "Etiology of inflammatory bowel disease", Curr Opin Gastroenterol, 6, pp.561~ (1990)
  5. Tiemey LM Jr, "Current Medical Diagnosis &Treatment", pp.686~695 (1999), Hanwoori
  6. Lennard-Jones JE, "Prednisone as maintenance treatment for ulcerative colitis in remission", Lancet, 1, pp.188~189 (1965)
  7. Kang TH, "Study on the treatment of Ulcerative Colitis in Oriental medicine", The Jounal of Oriental Chronic Diseases, 5(1), pp.137~162 (1999)
  8. Jiang YQ, "Integrative Medicine Subcommittee to study", pp.277~287 (1997), Beijing Publishing House
  9. Kook YB, "Effect of Hwangryunhaedoktang on Blood Pressure and Renal Functions in Spontaneously Hypertensive Rats", The Korean Jounal of Oriental Medical Prescription, 10(1), pp.113~129 (2002)
  10. 정재은, "黃連解毒湯이 저산소증에 의한 培養心筋細胞枯死에 미치는 영향", 동의생리병리학회지, 18(6), pp.1733~1739 (2004), 한의병리학회
  11. 조재용, "황련해독탕 약침액의 iNOS 생성 억제를 통한 항염증효과가 알레르기성 비염 유발 생쥐의 치료에 미치는 영향", 대한침구의학회지, 29(1), pp.89~101 (2012), 대한침구의학회
  12. 김영태, "天樞 상응부위에 灸津藥針 刺戟이 TNBS로 誘導된 크론병에 미치는 영향", Korean Journal of Acupuncture, 25(2), pp.159~177 (2008), 경락경혈학회
  13. 백대봉, "TNBS로 유도된 만성 염증성 대장염에 대한 大腸兪 灸津 藥鍼의 효과", Korean Journal of Acupuncture, 24(3), pp.149~164 (2007), 경락경혈학회
  14. 이경호, "감초 추출물의 Dextran Sulfate Sodium 유도 마우스 궤양성 대장염 억제효과", 한국식품영양학회지, 23(4), pp.435~439 (2010), 한국식품영양학회
  15. Song IS, "Inflammatory bowel disease in Korean", Korean J of Med, 57(4), pp.661~674 (1999)
  16. Yang SK, "Incidence and prevalence of ulcerative colitis in the Songpa-Kangdong District, Seoul, Korea, 1986-1997", J Gastroenterol Hepatol, 15(9), pp.1037~1042 (2000)
  17. Chang DK, "Follow-up of ulcerative colitis : short-term outcome to medical treatment and relapse rates", Korean J Gastroenterol, 26, pp.907~918 (1994)
  18. Song IS, "Current status of Crohn's disease in Korea", Korean J Intern Med, 55, pp.158~168 (1998)
  19. Kumar V, "ROBBINS Basic Pathology", pp.47~58 (2009), E-public
  20. Shin DH, "Increasing incidence of inflammatory bowel disease among young men in Korea between 2003and 2008", Dig Dis Sci, 56(4), pp.1154~1159 (2011)
  21. Kirsner JB, "Recent developments in `non specific’inflammatory bowel disease", N Engl Med, 306, pp.775~780 (1982)
  22. Korea medical medicine editorial department, "Digestive System diagnosis point", pp.100~ (1999), Korea medical book publisher
  23. The Korean Society of Neurogastroenterology and Motility, "Irritable Bowel Syndrome", pp.24~ (2011), The medbook co
  24. Glickman RM, "Inflammatory Bowel Disease : Ulcerative colitis &Crohn's disease in : Harrison's Principles Intern Med", pp.1268~1281 (1997), Book Publishing Jeongdam
  25. Dennis LK, "Harrison's Principles of Internal Medicine. 16th Ed", pp.1776~1788 (2005), McGraw-Hill
  26. Ge H, "Zhou hou bei ji fang", pp.34~37 (1996), People's health Publishers
  27. Kim GH, "Study on Analgesic, Anti-Inflammatory and Antipyretic Effects of Aqua-acupuncture and Admiminstration per orally of Whangryounhaedogtang and Onsungouhyoulbang", the Journals of Korean Oriental Medical Society, 15(1), pp.9~25 (1994)
  28. Sin MG Ed, "Clinical Traditional Herbalogy", pp.372~400 (2000), Younglim Inc
  29. Choi EG, "Effect of Hwangryeonhaedok -tang herb-acupuncture on functional headache", Journal of Oriental Neuropsychiatry, 11(1), pp.75~80 (2000)
  30. Hwang DY, "JeungMaek BangYakHapPyeon", pp.132~ (1990), Namsandang
  31. 임대환, "黃連解毒湯이 Dextran Sulfate Sodium 유도 마우스 대장염에 미치는 영향", 대한한의학 방제학회지, 19(2), pp.11~22 (2011), 대한한의학방제학회
  32. 안중환, "황련해독탕이 DSS로 유발된 흰쥐의 궤양성 대장염에 미치는 영향", 대한한의학회지, 27(2), pp.182~195 (2006), 대한한의학회
  33. Choe YT, "Acupuncture(1 part)", pp.371~372 (1991), Jibmundang
  34. 송정방, "消炎藥鍼이 대장염 유발 흰쥐의 c-Fos 단백 발현에 미치는 효과", Journal of Pharmacopuncture, 13(3), pp.47~62 (2010), 대한약침학회
  35. Seong NG, "A clinical evaluation of stimlation and alnalgesic mechanism based on chichimbub", Korean Institute of Oriental Medicine Letters, 3(1), pp.152~155 (1994)
  36. 최유나, "약침 주입 용량 및 자입 방법에 따른 자극량 차이 연구", 대한침구의학회지, 32(1), pp.89~95 (2015), 대한침구의학회

관련 논문AI추천

消炎藥鍼이 대장염 유발 흰쥐의 c-Fos 단백 발현에 미치는 효과김재효, 손인철, 송정방, 안성훈대한약침학회 · Journal of Pharmacopuncture2010 · 제13권 제3호Acetic-acid로 대장염을 유발한 생쥐에 투여한 만년환의 치료효과공원호, 김범회, 김원일대한한방내과학회 · 대한한방내과학회지2023 · 제44권 제6호消炎整腸湯이 DSS로 誘發된 생쥐의 궤양성 대장염에 미치는 影響류봉하, 송영근, 윤성우대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제2호DSS으로 유발된 생쥐의 대장점막손상에 대한 導滯湯의 효과공경환, 이주아대한한방내과학회 · 대한한방내과학회지2008 · 제29권 제3호烏梅丸과 烏梅가 DSS로 유발된 생쥐의 염증성 장 질환에 미치는 영향김의수, 김태련, 신만호, 신민구, 이영수대한한방내과학회 · 대한한방내과학회지2015 · 제36권 제3호흰쥐에서 acetic acid로 유발된 궤양성 대장염에 대한 만년환의 효과공원호, 김범회, 김원일대한한방내과학회 · 대한한방내과학회지2024 · 제45권 제4호

이 간행물의 다른 논문 (10건)

In Vitro Cytotoxicity, Skin Regeneration, Anti-wrinkle, Whitening and In Vivo Skin Moisturizing Effects of Oncheongeum안뜰에봄, 김동철pp. 14-34보허탕가감 투여가 산후 생쥐의 유즙분비량 및 유즙분비 관련 인자에 미치는 영향이창현, 김홍준, 이아영, 이은희, 임지영pp. 35-52한의사 교의(校醫)가 진행한 서울소재 일개 초등학교 학생 대상 성교육 프로그램의 효과이승환, 김동일, 김경한pp. 53-68중고등학교 여학생의 월경통 한방 치료 효과 및 만족도 분석박수정, 김정호, 이상덕, 김성배, 주종천pp. 69-77유방암 환자 대상 침치료 무작위비교임상연구 고찰장준복, 이윤재, 황덕상, 이승열, 정유화, 이성엽pp. 78-91
원문 보기