흰쥐에서 acetic acid로 유발된 궤양성 대장염에 대한 만년환의 효과
The Effects of Mannyeon-hwan on Acetic Acid-induced Ulcerative Colitis in Rats
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: This study was designed to verify the effects of Mannyeon-hwan (MNH) on acetic acid-induced ulcerative colitis in rats. Methods: Ulcerative colitis was induced in male Sprague-Dawley rats weighing approximately 250 g by injecting acetic acid through the anus. Rats were classified into four groups: normal group, control group (acetic-acid, AA), low concentration group (AA+MNH(L)), and high concentration group (AA+MNH(H)). Body weight, visual evaluation of the colonic mucosa, anatomical histological changes, and changes in the expression of proteins in colon tissue were compared and analyzed. Results: Compared to the control group, the MNH groups showed significant recovery from weight loss and mucosal damage. The expression of inflammatory proteins such as TNF-α, IL-6, IL-1β, NF-κB p65, MPO, and COX-2 were significantly decreased in the MNH groups compared to those in the AA group. In the MNH groups, significant changes in proteins involved in apoptosis such as caspase-3, BAX, and Bcl-2 were observed compared to those in the AA group. Additionally, changes in the expression of TNF-α, IL-6, IL-1β, NF-κB p65, BAX, and Bcl-2 were greater in the MNH(H) group than those in the MNH(L) group. Conclusion: Mannyeon-hwan was found to be effective in suppressing inflammation of the colonic mucosa in ulcerative colitis, inhibiting the expression of inflammation-related proteins, and suppressing damage to the colonic mucosa by regulating the expression of apoptosis-related proteins.
Abstract
Objectives: This study was designed to verify the effects of Mannyeon-hwan (MNH) on acetic acid-induced ulcerative colitis in rats. Methods: Ulcerative colitis was induced in male Sprague-Dawley rats weighing approximately 250 g by injecting acetic acid through the anus. Rats were classified into four groups: normal group, control group (acetic-acid, AA), low concentration group (AA+MNH(L)), and high concentration group (AA+MNH(H)). Body weight, visual evaluation of the colonic mucosa, anatomical histological changes, and changes in the expression of proteins in colon tissue were compared and analyzed. Results: Compared to the control group, the MNH groups showed significant recovery from weight loss and mucosal damage. The expression of inflammatory proteins such as TNF-α, IL-6, IL-1β, NF-κB p65, MPO, and COX-2 were significantly decreased in the MNH groups compared to those in the AA group. In the MNH groups, significant changes in proteins involved in apoptosis such as caspase-3, BAX, and Bcl-2 were observed compared to those in the AA group. Additionally, changes in the expression of TNF-α, IL-6, IL-1β, NF-κB p65, BAX, and Bcl-2 were greater in the MNH(H) group than those in the MNH(L) group. Conclusion: Mannyeon-hwan was found to be effective in suppressing inflammation of the colonic mucosa in ulcerative colitis, inhibiting the expression of inflammation-related proteins, and suppressing damage to the colonic mucosa by regulating the expression of apoptosis-related proteins.
참고문헌 (35)
- Ng WK, "Changing epidemiological trends of inflammatory bowel disease in Asia", Intest Res, 14(2), pp.111~119 (2016)
- Song EM, "Treatment of inflammatory bowel disease–Asian perspectives : the results of a multinational web-based survey in the 8th Asian Organization for Crohn’s and Colitis meeting", Intest Res, 21(3), pp.339~352 (2023)
- Ha JE, "Medication Use and Drug Expenditure in Inflammatory Bowel Disease : based on Korean National Health Insurance Claims Data(2010-2014)", Korean J Clin Pharm, 29(2), pp.79~88 (2019)
- Magro F, "Third European Evidence-based Consensus on Diagnosis and Management of Ulcerative Colitis. Part 1 : Definitions, Diagnosis, Extra-intestinal Manifestations, Pregnancy, Cancer Surveillance, Surgery, and Ileo-anal Pouch Disorders", J Crohns Colitis, 11(6), pp.649~670 (2017)
- Lee JH, "Liver enzyme abnormalities in taking traditional herbal medicine in Korea : A retrospective large sample cohort study of musculoskeletal disorder patients", J Ethnopharmacol, 169, pp.407~412 (2015)
- Lee BH, "Herbal medicine for inflammatory bowel disease : systematic review and exploratory practice algorithm through case series", pp.2016
- Department of gastrointestinal medicine Korean medicine, "Gastrointestinal internal medicine", pp.366~396 (2008), Gunja
- Heo G, "The Effects of Coicis Semen Extract(CSE)on Dextran Sulfate Sodium-Induced Colitis in Mice", J Int Korean Med, 33(4), pp.520~532 (2012)
- Kim ES, "Effect of Ohmae-hwan and Mume Fructus on DSS-Induced Inflammatory Bowel Disease in a Mice Model System", J Int Korean Med, 36(3), pp.284~296 (2015)
- Bae KH, "Effects of Goihwa-san on Ulcerative Colitis Induced by Dextran Sulfate Sodium in mice", J Int Korean Med, 31(3), pp.513~525 (2010)
- Choi JY, "Mitigating Effect of Jageum-jung on Dextran Sulfate Sodium-induced Ulcerative Colitis through Anti-inflammatory Regulation", J Int Korean Med, 38(6), pp.944~954 (2017)
- Choi HJ, "A Case of Ulcerative Colitis Diagnosed as Dual deficiency of the spleen-kidney Treated with Chojunggunbiwhan-gamibang", J Int Korean Med, 29(110), pp.251~256 (2008)
- Choi SW, "A Case of Ulcerative Colitis Treated with Goihwasan and Gami-Jeonssibackchulsan", J Int Korean Med, 34(104), pp.157~163 (2013)
- Kong WH, "Effects of Mannyeon-hwan on Acetic Acid-induced Colitis in Rats", J Int Korean Med, 44(6), pp.1139~1149 (2023)
- Choi SH, "Effects of Paeoniae Radix, Glycyrrhiza Uralensis and Jakyakgamcho-tang Treatment on Ulcerative Colitis Animal Model : Including Changes in Metabolites", The Journal of Pediatrics of Korean Medicine, 36(3), pp.19~34 (2022)
- Chen Z, "Therapeutic Effect of Amomum villosum on Inflammatory Bowel Disease in Rats", Front Pharmacol, 9, pp.639~ (2018)
- Park SM, "Antioxidant Effect of Atractylodes macrocephala Koidzumi in DSS-induced Ulcerative Colitis Model", The Korea Journal of Herbology, 37(1), pp.19~29 (2022)
- Yazaki Y, "Asakura Internal medicine", pp.96~114 (2020), Woori Medical Books
- Langholz E, "Course of ulcerative colitis : analysis of changes in disease activity over years", Gastroenterology, 107(1), pp.3~11 (1994)
- Yang, "Epidemiology of inflammatory bowel disease in the Songpa-Kangdong district, Seoul, Korea, 1986-2005 : a KASID study", Inflamm Bowel Dis, 14(4), pp.542~549 (2008)
- Choi CH, "Second Korean Guideline for the Management of Ulcerative Colitis", Korean J Gastoenterol, 69(1), pp.1~28 (2017)
- Bae WS, "Hanbangimsanghak" (1976), Namsandang
- Bonchohak co-editing committee, "Bonchohak" (2020), Younglimsa
- Oriental Pharmacology Textbook Compilation Committee, "Oriental Pharmacology" (2006), Shinil Corporation
- Jang BI, "Animal Models of Inflammatory Bowel Disease", Intest Res, 6(1), pp.8~18 (2008)
- Yamada Y, "A comparative analysis of two models of colitis in rats", Gastroenterology, 102(5), pp.1524~1534 (1992)
- Bruner LP, "Inflammatory Bowel Disease", Prim Care, 50(3), pp.411~427 (2023)
- Strober W, "Proinflammatory cytokines in the pathogenesis of inflammatory bowel diseases", Gastroenterology, 140(6), pp.1756~1767 (2011)
- Schottelius AJ, "Cytokines, NF-kappaB, microenvironment, intestinal inflammation and cancer", Cancer Treat Res, 130, pp.67~87 (2006)
- Neurath MF, "Pathogenesis of inflammatory bowel disease : transcription factors in the spotlight", Gut, 42(4), pp.458~459 (1998)
- Aratani Y, "Myeloperoxidase : Its role for host defense, inflammation, and neutrophil function", Arch Biochem Biophys, 640, pp.47~52 (2018)
- Kawamori T, "Chemopreventive activity of celecoxib, a specific cyclooxygenase-2 inhibitor, against colon carcinogenesis", Cancer Res, 58(3), pp.409~412 (1998)
- Becker C, "Complex Roles of Caspases in the Pathogenesis of Inflammatory Bowel Disease", Gastroenterology, 144(2), pp.283~293 (2013)
- Wan Y, "Excessive Apoptosis in Ulcerative Colitis : Crosstalk Between Apoptosis, ROS, ER Stress, and Intestinal Homeostasis", Inflamm Bowel Dis, 28(4), pp.639~648 (2022)
- Wang Q, "The Relationship between the Bcl-2", Bax Proteins and the Mitochondria-Mediated Apoptosis Pathway in the Differentiation of Adipose-Derived Stromal Cells into Neurons, 2016, pp.11(10), e0163327~