함양산양삼과 열매 복합물의 항산화 및 항염증 효능에 대한 연구
A Study on the Antioxidant and Anti-inflammatory Effects of Hamyang Wild-Simulated Ginseng and Ginseng Berry Complex
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The purpose of this study is to examine the antioxidant effect of hamyang wild-simulated ginseng and ginseng berry complex(HGC) and to investigate the mechanism of anti-inflammatory effect on macrophage cell line (RAW264.7 cells). For antioxidant activity of HGC was measured by total polyphenol contents were estimated with folin-ciocalteu method, and total flavonoid contents were estimated with aluminum chloride methods. And, assessed with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity assay. MTT assay was performed to determine the effect of HGC on cell viability in RAW264.7 cells. In addition, anti-inflammatory effect of HGC was investigated in RAW264.7 cells. RAW 264.7 cells, in which an inflammatory response was induced by lipopolysarccharide (LPS), were treated with HGC, and nitric oxide production was measured by griess assay. And protein expression level of inflammatory cytokine and phosphorylation of MAPK signaling pathways were confirmed by western blot analysis. Total polyphenols and flavonoids contents in HGC were quantified, and increased DPPH and ABTS radical scavenging activity in a concentration-dependent manner was confirmed. In addition, HGC was significantly inhibited nitric oxide (NO) production and inflammatory cytokine (iNOS, IL-1β, IL-6, and TNF-α) expression in LPS-stimulated RAW264.7 cells. Also, HGC was significantly inhibited the phosphorylation of MAPK signaling pathways. These results suggest that HGC can be used as effective herbal medicine extract for antioxidant and anti-inflammatory effect. As a result, it is thought that HCG can be used as a functional material effective for antioxidant and anti-inflammatory effects. And it will be possible to promote industrialization using hamyang wild-simulated ginseng and ginseng berry, and to increase high-value-added.
Abstract
The purpose of this study is to examine the antioxidant effect of hamyang wild-simulated ginseng and ginseng berry complex(HGC) and to investigate the mechanism of anti-inflammatory effect on macrophage cell line (RAW264.7 cells). For antioxidant activity of HGC was measured by total polyphenol contents were estimated with folin-ciocalteu method, and total flavonoid contents were estimated with aluminum chloride methods. And, assessed with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity assay. MTT assay was performed to determine the effect of HGC on cell viability in RAW264.7 cells. In addition, anti-inflammatory effect of HGC was investigated in RAW264.7 cells. RAW 264.7 cells, in which an inflammatory response was induced by lipopolysarccharide (LPS), were treated with HGC, and nitric oxide production was measured by griess assay. And protein expression level of inflammatory cytokine and phosphorylation of MAPK signaling pathways were confirmed by western blot analysis. Total polyphenols and flavonoids contents in HGC were quantified, and increased DPPH and ABTS radical scavenging activity in a concentration-dependent manner was confirmed. In addition, HGC was significantly inhibited nitric oxide (NO) production and inflammatory cytokine (iNOS, IL-1β, IL-6, and TNF-α) expression in LPS-stimulated RAW264.7 cells. Also, HGC was significantly inhibited the phosphorylation of MAPK signaling pathways. These results suggest that HGC can be used as effective herbal medicine extract for antioxidant and anti-inflammatory effect. As a result, it is thought that HCG can be used as a functional material effective for antioxidant and anti-inflammatory effects. And it will be possible to promote industrialization using hamyang wild-simulated ginseng and ginseng berry, and to increase high-value-added.
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