Intramuscular Single-dose Toxicity Test of Bufonis venonum Pharmacopuncture in Sprague-Dawley Rats
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Objectives: Bufonis venonum (BV) is the dried whitesecretions of the auricular and skin glands of the toadsBufo bufo gargarizans or Bufo melanosticus Schneider. This study was performed to evaluate the toxicity of intramuscularly-administered Bufonis venonum pharmacopuncture(BVP) and to calculate its approximatelethality through a single-dose test with Sprague-Dawley(SD) rats. Methods: Twenty male and 20 female 6-week-old SDrats were injected intramuscularly with BVP or normalsaline. The animals were divided into four groups withfive female and five male rats per group: the controlgroup injected with normal saline at 0.5 mL/animal,the low-dosage group injected with 0.125 mL/animalof BVP, the medium-dosage group injected with 0.25mL/animal of BVP and the high-dosage group injectedwith 0.5 mL/animal of BVP. All injections were in the leftthighs of the rats. After administration, we conductedclinical observations everyday and body weight measurementson days 3, 7 and 14 after the injection. We alsocarried out hematology, serum biochemistry, and histologicalobservations on day 15 after treatment. Results: No mortalities were observed in any experimentalgroup. No significant changes in weight, hematology,serum biochemistry, and histological observationsthat could be attributed to the intramuscularinjection of BVP were observed in any experimentalgroup. Conclusion: Lethal dose of BVP administered via intramuscularinjection in SD rats is over 0.5 mL/animal.
Abstract
Objectives: Bufonis venonum (BV) is the dried whitesecretions of the auricular and skin glands of the toadsBufo bufo gargarizans or Bufo melanosticus Schneider. This study was performed to evaluate the toxicity of intramuscularly-administered Bufonis venonum pharmacopuncture(BVP) and to calculate its approximatelethality through a single-dose test with Sprague-Dawley(SD) rats. Methods: Twenty male and 20 female 6-week-old SDrats were injected intramuscularly with BVP or normalsaline. The animals were divided into four groups withfive female and five male rats per group: the controlgroup injected with normal saline at 0.5 mL/animal,the low-dosage group injected with 0.125 mL/animalof BVP, the medium-dosage group injected with 0.25mL/animal of BVP and the high-dosage group injectedwith 0.5 mL/animal of BVP. All injections were in the leftthighs of the rats. After administration, we conductedclinical observations everyday and body weight measurementson days 3, 7 and 14 after the injection. We alsocarried out hematology, serum biochemistry, and histologicalobservations on day 15 after treatment. Results: No mortalities were observed in any experimentalgroup. No significant changes in weight, hematology,serum biochemistry, and histological observationsthat could be attributed to the intramuscularinjection of BVP were observed in any experimentalgroup. Conclusion: Lethal dose of BVP administered via intramuscularinjection in SD rats is over 0.5 mL/animal.
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