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宮頸1號煎이 子宮頸部癌細胞(HeLa Cell)에 미치는 影響

Kung-Kyung-IlHo-Jeon on Induced Apoptosis in Human CervicalCarcinoma HeLa Cells

최창민|조한백|유심근|강영금
대한한방부인과학회 (The Society Of Oriental Obstetrics & Gynecology)|2005.02|대한한방부인과학회지 (The Journal of Oriental Obstetrics ＆ Gynecology)|제18권 제1호|pp. 15-28

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주제분류한의학

초록

Abstract

To address the ability of Kung-Kyung-Ilho-Jeon(KK) to induce cell death, we investigated the effect of KK on cell viability. Forty-eight hours later, loss of viability occurred following KK exposure in a dose-dependent manner. The treatment of KK, a commonly used herb formulation in Korea and China, caused a decrease in cell viability. KK also resulted in apoptotic morphology a brightly blue-fluorescent condensed nuclei by Hoechst 33258-staining, and reduction of cell volume. Our results show that KK induces caspase-3 and -9 activation in a time-dependent manner. In addtion, the translocation of cytochrome c release into cytoplasm has been observed under the presence of 5 ㎎/㎖ KK. The subsequent loss of mitochondria membrane potential is collapsed by the addition of KK. Our immunoblotting data show that PARP, a well known caspase-3 and -6 substrate, is cleaved by KK. We show that a pro-apoptotic protein, Bax is increased in the presence of KK but that the amount of Bcl-2 is not changed. We suggest that Bax, a critical protein which can regulate channel of mitochondria to release cytochrome c, is a key protein in KK-induced apoptosis of Hela human cervical carcinoma cells

참고문헌 (46)

  1. "암 동서의결합치료", pp.379~421 (1999), 圓光大學校出版局
  2. "韓醫婦人科學(상)", pp.319~26 (2001), 정담
  3. "韓方婦人科學", pp.249~57 (1998), 행림출판
  4. "韓方婦人科 臨床診療", pp.264~ (1982), 癸丑文化社
  5. "자궁경부암의 발암과정과 세포자연사의 연관성", 10(2), pp.138~47 (1999)
  6. "distinct modes of cell death with fundamentally different significance", pp.17 229~59 (1982)
  7. "加味溫經湯이 子宮頸部癌細胞(HeLa Cell)에 미치는 影響" (2001), 圓光大學校 大學院
  8. "濟川煎이 子宮頸部癌細胞(HeLa Cell)에 미치는 影響", 16(2), pp.161~76 (2003)
  9. "六合湯이 子宮頸部癌細胞(HeLa Cell)에 미치는 影響", pp.128~46
  10. "癌症秘方驗方偏方大全", pp.386~ (1992), 中國醫藥科技出版社
  11. "한방임상종양학", pp.799~818 (2001), 주민출판사
  12. "中西醫臨床腫瘤學", pp.869~ (1996), 중국중의약출판사
  13. "抗癌中藥一千方", pp.424~ (1996), 中國醫藥科技出版社
  14. "抗癌 中藥方選", pp.181~ (1993), 人民軍醫出版社
  15. "assay for protein quantitation", pp.5~8 (1994), The bicinchoninic acid
  16. "elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme", pp.641~52 (1993)
  17. "death receptors and cytotoxic drugs in cancer therapy", 7(2), pp.95~106 (2001)
  18. "Bax translocation to mitochondria subsequent to a rapid loss of mitochondrial membrane potential", 8, pp.909~20 (2001)
  19. "Biochemical and genetic analysis of the mitochondrial response of yeast to Bax and Bcl-xL", 20, pp.3125~36 (2000)
  20. "Mitochondria permeability transition is a central codinating event of apoptosis", 184, pp.1155~60 (1996)
  21. "A target for apoptosis regulation by caspases and Bcl-2 -related proteins", 187, pp.1261~71 (1998)
  22. "Caspase-dependent apoptosis of COS-7 cells induced by Bax overexpression differential effects of Bcl-2 and Bcl-XL on Bax-induced caspase activation and apoptosis", 15, pp.1763~72 (1997)
  23. "Bax indirectly induces release of cytochrome c from isolated mitochondria", 95, pp.4997~5002 (1998)
  24. "The overexpression of Bax produces cell death upon induction of the mitochondrial permeability transition", 273, pp.7770~5 (1998)
  25. "Cleavage of caspase-1 -9 substrates by proteases in skeletal muscles from mice undergoing cancer cachexia", 84(8), pp.1135~40 (2001)
  26. "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death", pp.334~6 (1990)
  27. "Double identity for proteins of the Bcl-2 family", 387, pp.773~6 (1997)
  28. "Possible involvement of both endoplasmic reticulum-and mitochondria-dependent pathways in thapsigargin-induced apoptosis in human neuroblastoma in SH-SY5Y cells", 92(3), pp.228~36 (2003)
  29. "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta", 403(6765), pp.98~103 (2000)
  30. "자궁경부암에서 Bcl-2, Bax, VEGF, PCNA 단백의 발현 및 apoptosis 양상과 선행항암화학요법 반응도와의 상관관계에 관한 연구", 11(1), pp.24~37 (2000)
  31. "의 治方에 對한 文獻的 考察", 6(2), pp.417~35 (1998)
  32. "巢氏諸病源候論", pp.150~2 (1992), 大星文化社
  33. "華陀神醫秘傳", pp.20~ (1976), 東醫輔
  34. "編註醫學入門 婦人門", pp.40~3 (1990), 大星文化社
  35. "校注婦人良方注釋", pp.157~60 (1989), 一中社
  36. "張氏景岳全書", pp.734~8 (1983), 成輔社
  37. "醫林改錯評譯", pp.129~32 (1998), 成輔社
  38. "자궁경부 상피내 종양 및 자궁경부암 조직에서 p53, MIB-1, 및 세포고사(apoptosis)에 관한 연구", 44(6), pp.1115~22 (2001)
  39. "Dexamethasone suppresses tumor necrosis factor-alpha-induced apoptosis in osteoblast possible role for ceramide", 141(8), pp.2904~13 (2000)
  40. "Fas antigen modulates ultraviolet B-induced apoptosis of SVHK cells sequential activation of caspase 8 and 1 in the apoptotic process", 242(2), pp.291~8 (1999)
  41. "A novel target for zinc in the inhibition of apoptosis", 272, pp.~3 (1997), Zinc is a potent inhibitor protease
  42. "Caspase-dependent clevage of signaling proteins during apoptosis A turn-off mechanism for anti-apoptotic signals", 273(12), pp.7141~7 (1998)
  43. "Heterodimerization of Bcl-2 and Bcl-X with Bax and Bad in colorectal cancer", 39(4), pp.495~500 (2000)
  44. "Susceptibility to drug-induced apoptosis correlates with differential modulation of Bad Bcl-2 and Bcl-xL protein levels", 7(6), pp.574~86 (2000)
  45. "Bcl-2 heterodimerizes in vivo with a conserved homolog Bax that accelerates programmed cell death", pp.609~19 (1993)
  46. "Prevention of apoptosis by Bcl-2 release of cytochrome c from mitochondrial blocked", 275, pp.1129~32 (1997)

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