六鬱湯에 의한 인체자궁경부암세포의 증식억제에 관한 연구
Induction of Apoptosis by Yukwool-tangin Human Cervical Carcinoma HeLa Cells
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Abstract
Yukwool-tang (YWT) is a traditional Chinese medicine, which has been used for patients suffering from a uterine disease in Oriental medicine. In the present study, it was examined the biochemical mechanisms of apoptosis by YWT in human cervical carcinoma HeLa cells. It was found that YWT could inhibit the cell growth of HeLa cells in a dose-dependent manner, which was associated with apoptotic cell death such as formation of apoptotic bodies and DNA fragmentation. Flow cytometry analysis confirmed that YWT treatment increased populations of apoptotic-sub-G1 phase of the cell cycle. We observed the p53-independent induction of p21 proteins, down-regulation of anti apoptotic Bcl-2 expression and proteolytic activation of caspase-3 in YWT-treated HeLa cells. YWT treatment also concomitant degradation and/or inhibition of poly (ADP-ribose) polymerase (PARP), phospholipase C-1 (PLCγ1), β-catenin and DNA fragmentation factor 45/inhibitor of caspase-activated DNase (DFF45/ICAD). Taken together, these findings partially provide novel insights into the possible molecular mechanism of the anti-cancer activity of YWT.
참고문헌 (24)
- Arends, "A.H. Apoptosis. The role of the endonuclease. Am. J. Pathol. 136", pp.593~608 (1990)
- Evans, "V.G. Multiple pathways to apoptosis. Cell Biol. Int. 17", pp.461~476 (1993)
- "A.H. Premature p34cdc2 activation required for apoptosis. Science 263", pp.1143~1145 (1994)
- "B. WAF1", CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 54, 1994., 1169~1174
- "Development of health assistances for anti-stress used with Ostreae concha", 20, pp.1604~1611 (2006)
- "S.J. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75", pp.805~816 (1993)
- "D. p21 is a universal inhibitor of cyclin kinases. Nature 366", pp.701~704 (1993)
- "on the threshold of checkpoints and development. Curr. Opin. Cell Biol. 6", pp.847~852 (1994)
- "Tumor suppressor p53 is a direct transcriptional activator of the human bax gene", 80, pp.293~299 (1995)
- "Principles of CDK regulation", 374, pp.131~134 (1995)
- "Bcl-2 family proteins", 17, pp.3225~3236 (1998)
- "Implication of alternative splice transcripts of caspase-3 and survivin in chemoresistance", 92, pp.219~226 (2005)
- "G.G. Specific proteolytic cleavage of poly an early marker of chemotherapy-induced apoptosis. Cancer Res. 53", ottavia, pp.3976~3985 (1993)
- "Yama", CPP32, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase, 1995, pp.81, 801~809
- "Apoptosis-associated cleavage of β-catenin in human colon cancer and rat hepatoma cells", 31, pp.519~529 (1999)
- "Apoptosis-induced cleavage of β-catenin by caspase-3 results in proteolytic fragments with reduced transactivation potential", 275, pp.16345~16353 (2000)
- "S.Y. Studies of inositol phospholipid-specific phospholipase C. Science 244", pp.546~550 (1989)
- "Proteolytic cleavage of phospholipase C-gamma1 during apoptosis in Molt-4 cells", 14, pp.1083~1092 (2000)
- "Discovery, regulation, and action of the major apoptotic nucleases DFF40", CAD and endonuclease G, 2005, pp.94, 1078~1087
- "Apoptotic DNA fragmentation", 256, pp.12~18 (2000)
- "Death receptors as targets of cancer therapeutics", 4, pp.97~104 (2004)
- "P.H. Activation interferes with the APO-1 pathway in mature human T cells. Int. Immunol. 5", pp.625~630 (1993)
- "Current strategies to target the anti-apoptotic Bcl-2 protein in cancer cells", 11, pp.1031~1039 (2004)
- "Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c", 391, pp.496~499 (1998)